Team:WarrenCIndpls IN-HS/Project

From 2011.igem.org

(Difference between revisions)
(The Experiment)
Line 44: Line 44:
=== The Experiment ===
=== The Experiment ===
'''DNA Extraction'''
'''DNA Extraction'''
-
1) Add 200 microliters of distilled water and a drop of detergent to a centrifuge tube; then shake (twice)
+
1) Pipet 200 microliters of distilled water into two centrifuge tubes
-
2) Inoculate a sample of E.Coli  
+
2)  Add a drop of detergent to each tube and shake.
 +
3) Label the tubes according to what will go into each.
 +
4) Rub the loop end of the inoculating loop across the surface of the agar with the E.Coli containing the ADH terminator.
 +
5)  Place the loop into the corresponding  tube and swivel the loop a few times.
 +
6)  Repeat 4-5 with the E.Coli containing M-Cherry.
 +
7)  Shake the tubes well or place them on a vortex for about 5 seconds.
 +
8)  Add a pinch of meat tenderizer to each tube.
 +
    -this will break apart the proteins holding the cells together.
 +
9)  Vortex the solutions again.
 +
10) Place them in a water bath at 56-60 degrees Celsius
 +
 
=== Part 3 ===
=== Part 3 ===
== Results ==
== Results ==

Revision as of 04:39, 20 June 2011

WarrenCIndpls IN-HS logo.png

Some bacteria have been genetically modified to detect certain metals in a water supply. However, it has not been done in yeast. If yeast is genetically engineered to do so, it would be a much safer procedure. The objective for was to create a bio-brick. The creation of the part will enable next year's team to complete the overall goal, which is to create and insert a plasmid into yeast that will cause it to glow red in the presence of a certain metal.


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions



Contents

Overall project

Project Details

Gibson Assembly

The Experiment

DNA Extraction 1) Pipet 200 microliters of distilled water into two centrifuge tubes 2) Add a drop of detergent to each tube and shake. 3) Label the tubes according to what will go into each. 4) Rub the loop end of the inoculating loop across the surface of the agar with the E.Coli containing the ADH terminator. 5) Place the loop into the corresponding tube and swivel the loop a few times. 6) Repeat 4-5 with the E.Coli containing M-Cherry. 7) Shake the tubes well or place them on a vortex for about 5 seconds. 8) Add a pinch of meat tenderizer to each tube.

    -this will break apart the proteins holding the cells together.

9) Vortex the solutions again. 10) Place them in a water bath at 56-60 degrees Celsius

Part 3

Results