Team:WarrenCIndpls IN-HS/Notebook

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Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


Contents

Notebook

May 4th: Bacterial and Yeast Transformation

May 11th: Gibson Assembly

Gibson Assembly pdf

May 19th: Research on Parts

Kozak Sequence- directs translation of mRNA for more efficiency and accuracy

Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors

Terminator Sequence- signals the end of transcriptoin to RNA polymerase

Vector- Plasmid

    ORI (Origin of Replication)
    Selection Markers
         -Ura3 is a selection marker for yeast
         -Ampicillin Resistance is a selection marker for bacteria based antibiotic resistance

May 26th: Primer Design