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Data for our favorite new parts
BBa_K546546 - The Synchroscillator: Our goal was to achieve synchronous oscillatory behavior in a population of E. coli cells. Our data shows that this has been achieved. Moreover, we have created the system in such a way that it is theoretically tuneable in two ways.
BBa_K546005 - Quorum-induced positive feedback loop quantifiable by GFP: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of autoinducer synthetase. The system creates a positive feedback loop, yielding high titres of GFP.
BBa_K546001 - 3OC6HSL Receiver Device with a regulated 3OC6HSL inactivation enzyme gene: AHL responsive element that produces a low constitutive level of luxR via pL. Combined with 3-oxohexanoyl-homoserine lactone, luxR induces pR. lux pR regulates the autoinducer inactivation enzyme A (aiiA) gene that degrades AHL.
Data for pre-existing parts
BBa_I0460 - aiiA Device: Autoinducer inactivation enzyme A (AiiA) is capable of degrading the quorum sensing molecule AHL.
BBa_K082014 - Autoinducer synthethase: Produces the quorum sensing molecule 3-oxohexanoyl-homoserine lactone (AHL).
BBa_I763020 - GFP + LVA Tag Green fluorescent protein with a degradation tag fused that cares for a shorter lifetime of the protein.
Other new parts
BBa_K546002 - 3OC6HSL Receiver Device with a lux pR regulated GFP (including a LVA Tag) Reporter: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of GFP. Upon addition of AHL the GFP expression increases.
BBa_K546000 - Autoinducer 3OC6HSL Receiver Device with a lux pR Controlled Autoinducer Synthetase: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of autoinducer synthetase.
BBa_K546003 - 3OC6HSL Receiver Device with lux pR: 3-oxohexanoyl-homoserine lactone (AHL) responsive element that constitutively produces an intermediate level of LuxR via lux pL promoter. Combined with AHL, luxR activates lux pR promoter. This part is a repaired version of BBa_F2621 that appeared to be defective (R0063 missing), when we sequenced the part.
