Team:Wageningen UR/Project/PartsProj1

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Building a Synchronized Oscillatory System

Synchroscillator: Data page/parts submitted to the registry

Parts Submitted to the Registry

BBa_K546003 - 3OC6HSL Receiver Device with lux pR: 3-oxohexanoyl-homoserine lactone (AHL) responsive element that produces a low constitutive level of luxR via lux pL. Combined with 3-oxohexanoyl-homoserine lactone, luxR induces lux pR.


BBa_K546002 - 3OC6HSL Receiver Device with a lux pR Regulated GFP (including a LVA Tag) Reporter: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of GFP. Upon addition of AHL the GFP expression increases.


BBa_K546000 - Autoinducer 3OC6HSL Receiver Device with a lux pR Controlled Autoinducer Synthetase: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of autoinducer synthetase.


BBa_K546005 - Autoinducer 3OC6HSL Receiver Device with a lux pR Regulated GFP (including a LVA Tag) & Autoinducer Synthetase: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of autoinducer synthetase. The system creates a positive feedback loop, yielding high titres of GFP.