Team:Virginia/Parts

From 2011.igem.org

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body {
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!align="center"|[[Team:Virginia|Home]]
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!align="center"|[[Team:Virginia/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Virginia Official Team Profile]
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!align="center"|[[Team:Virginia/Project|Project]]
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!align="center"|[[Team:Virginia/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Virginia/Modeling|Modeling]]
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!align="center"|[[Team:Virginia/Notebook|Notebook]]
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!align="center"|[[Team:Virginia/Safety|Safety]]
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!align="center"|[[Team:Virginia/Attributions|Attributions]]
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===Parts===
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New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
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<groupparts>iGEM010 Virginia</groupparts>
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<div class="toplogo"><a href="https://2011.igem.org/Team:Virginia"><img src="https://static.igem.org/mediawiki/2011/8/89/Emergent_Chaos2.jpg" width="909" height="270" border="0" /></a></div>
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<ul class="bannerNav">
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<li><a href="https://2011.igem.org/Team:Virginia/Project" >PROJECT</a></li>
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                    <li><a href="#" >PARTS</a></li>
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                    <li><a href="https://2011.igem.org/Team:Virginia/Modeling" >MODELING</a></li>
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                    <li><a href="http://openwetware.org/wiki/IGEM:Virginia/2009/Notebook/VGEM2011/2011" target="_blank">NOTEBOOK</a></li>
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                    <li><a href="https://2011.igem.org/Team:Virginia/Safety" >SAFETY</a></li>
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                    <li><a href="https://2011.igem.org/Team:Virginia/HumanPractices" >HUMAN PRACTICES</a></li>
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                    <li><a href="https://2011.igem.org/Team:Virginia/Resources" >RESOURCES</a></li>
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                    <li><a href="https://2011.igem.org/Team:Virginia/Team" >TEAM</a></li>
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                    <li><a href="https://2011.igem.org/Team:Virginia/Attributions" >ATTRIBUTIONS</a></li>
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<div class="container">
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    <h1>Parts</h1>
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    <p>We submitted three biobrick parts to the parts registry: a yeast plasmid backbone, PDGF-B and siRNA silencing</p>
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    <h2>Composite coding region for PDGF-β in yeast (<a href="http://partsregistry.org/Part:BBa_K635001">BBa_K635001</a>)</h2>
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    <p>This part contains a platelet derived growth factor (PDGF) sequence modified for effective expression within a yeast chassis. The part contains the cDNA sequence for PDGF codon-optimized for yeast expression. It also contains the biobrick kozak sequence BBa_J63003. Preceding the kozak sequence is a random buffer sequence complementary to an siRNA inhibitor designed for modular inhibition of translation in yeast. The siRNA sequence (BBa_K635000) is reverse complementary to both the kozak sequence and the random buffer. This idea was derived from the 2010 Stanford team's modular siRNA regulation in <em>E.coli.</em> Flanking the cDNA sequence is also a poly-A tail necessary for expression in eukaryotic hosts, such as yeast, to prevent degradation of the mRNA fragment. Following the poly-A tail is the biobrick BBa_K392003 yeast transcriptional terminator. Together these preparatory components allow for expression of PDGF within a yeast chassis.</p>
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    <h2>Artificial Yeast Chromosome (Ura3 &amp; Amp) (<a href="http://partsregistry.org/Part:BBa_K635002">BBa_K635002</a>)</h2>
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    <p>This part serves as a foundational contribution to expand the registry of yeast components. It is an easy to use artificial yeast chromosome with an auxitrophic uracil selection method for yeast expression, as well as an ampicillin selection for propagation in <em>E.coli.</em> The part was modified for biobrick compatibility from a working pRS316 plasmid containing a Gal1/10 bidirectional promoter which was removed through restriction digest. In addition to the biobrick prefix and suffix, the backbone contains Kpn1 and Sac1 restriction sites the flank the prefix and suffix respectively.</p>
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    <p>Yeast transformants can be selected by using a uracil drop out medium and<em> E.coli</em> transformants may be selected using ampicillin resistance.</p>
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<h2>siRNA gene silencer for use in yeast (<a href="http://partsregistry.org/Part:BBa_K635000">BBa_K635000</a>)</h2>
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    <p>This siRNA fragment is designed for modular inhibition of translation for various synthetic biology systems in yeast. The siRNA is reverse complementary to the biobrick kozak sequence BBa_J63003 and a randomly generated buffer sequence that should precede it GGAGTTGTGGGCACCTGCTA. The idea is similar to the RSID system developed by the Stanford 2010 iGEM team for siRNA control in <em>E.coli.</em></p>
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    <p>&nbsp;</p>
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Revision as of 19:24, 28 September 2011

iGEM - Team Virginia - Parts

Parts

We submitted three biobrick parts to the parts registry: a yeast plasmid backbone, PDGF-B and siRNA silencing

Composite coding region for PDGF-β in yeast (BBa_K635001)

This part contains a platelet derived growth factor (PDGF) sequence modified for effective expression within a yeast chassis. The part contains the cDNA sequence for PDGF codon-optimized for yeast expression. It also contains the biobrick kozak sequence BBa_J63003. Preceding the kozak sequence is a random buffer sequence complementary to an siRNA inhibitor designed for modular inhibition of translation in yeast. The siRNA sequence (BBa_K635000) is reverse complementary to both the kozak sequence and the random buffer. This idea was derived from the 2010 Stanford team's modular siRNA regulation in E.coli. Flanking the cDNA sequence is also a poly-A tail necessary for expression in eukaryotic hosts, such as yeast, to prevent degradation of the mRNA fragment. Following the poly-A tail is the biobrick BBa_K392003 yeast transcriptional terminator. Together these preparatory components allow for expression of PDGF within a yeast chassis.

Artificial Yeast Chromosome (Ura3 & Amp) (BBa_K635002)

This part serves as a foundational contribution to expand the registry of yeast components. It is an easy to use artificial yeast chromosome with an auxitrophic uracil selection method for yeast expression, as well as an ampicillin selection for propagation in E.coli. The part was modified for biobrick compatibility from a working pRS316 plasmid containing a Gal1/10 bidirectional promoter which was removed through restriction digest. In addition to the biobrick prefix and suffix, the backbone contains Kpn1 and Sac1 restriction sites the flank the prefix and suffix respectively.

Yeast transformants can be selected by using a uracil drop out medium and E.coli transformants may be selected using ampicillin resistance.

siRNA gene silencer for use in yeast (BBa_K635000)

This siRNA fragment is designed for modular inhibition of translation for various synthetic biology systems in yeast. The siRNA is reverse complementary to the biobrick kozak sequence BBa_J63003 and a randomly generated buffer sequence that should precede it GGAGTTGTGGGCACCTGCTA. The idea is similar to the RSID system developed by the Stanford 2010 iGEM team for siRNA control in E.coli.

 

Copyleft. All rights reversed.

Retrieved from "http://2011.igem.org/Team:Virginia/Parts"