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Team:Uppsala-Sweden/Project/Protocol
From 2011.igem.org
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<div class="prefix_4 grid_11 suffix_1 maincontent" id="Uppsalacontent"> | <div class="prefix_4 grid_11 suffix_1 maincontent" id="Uppsalacontent"> | ||
== '''Protocol''' == | == '''Protocol''' == | ||
| - | + | ||
| + | Our protocols are provided here in the order they were implemented. | ||
| + | |||
| + | hyperlinks here | ||
| + | |||
| + | Buffers, medium and solutions | ||
| + | |||
| + | Preparing chemically competent TOP10 cells | ||
| + | |||
| + | Tranformation of TOP10 | ||
| + | |||
| + | Inoculation | ||
| + | |||
| + | Plasmid extraction | ||
| + | |||
| + | 3A assembly | ||
| + | |||
| + | Agarose gel electrophoresis | ||
| + | |||
| + | PCR protocols | ||
| + | |||
| + | Gibson Assembly | ||
Revision as of 08:53, 5 July 2011
Protocol
Our protocols are provided here in the order they were implemented.
hyperlinks here
Buffers, medium and solutions
Preparing chemically competent TOP10 cells
Tranformation of TOP10
Inoculation
Plasmid extraction
3A assembly
Agarose gel electrophoresis
PCR protocols
Gibson Assembly
