Team:Uppsala-Sweden/Project/Data

Overview of the system

Data For Our Favorite New Parts

1. Main Page (http://partsregistry.org/wiki/index.php?title=Part:BBa_K592009) - amilCP, a blue chromoprotein, BBa_K592009. This chromoprotein from the coral Acropora millepora, amilCP, naturally exhibits strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation.

2. Main Page (http://partsregistry.org/wiki/index.php?title=Part:BBa_K592100) - Blue Fluorescent Protein (mTagBFP), BBa_K592100. This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm.

3. Main Page (http://partsregistry.org/wiki/index.php?title=Part:BBa_K592200) - Low to medium copy BioBrick standard vector, BBa_K592200. A BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli.

Data For Pre-existing Parts

1. Main Page (http://partsregistry.org/Part:BBa_R0082) - Promoter (OmpR, positive), BBa_R0082. In nature, this promoter is upstream of the ompC porin gene.

2. Main Page (http://partsregistry.org/Part:BBa_Q04400) - QPI (B0034.C0040.B0015.R0040), BBa_Q04400 TetR QPI with strong RBS

3. Main Page (http://partsregistry.org/Part:BBa_J23113) - constitutive promoter family member, BBa_J23113 Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library.

4. Main Page (http://partsregistry.org/Part:BBa_I15008) - heme oxygenase (ho1) from Synechocystis, BBa_I15008 ho1 oxidizes the heme group using a ferredoxin cofactor, generating biliverdin IXalpha and representing the first of two steps in phycocyanobilin (PCB) biosynthesis. A review from the old result system indicates that this part did not work in some test.

5. Main Page (http://partsregistry.org/Part:BBa_I15009) - phycocyanobilin:ferredoxin oxidoreductase, BBa_I15009 Converts biliverdin IXalpha (BV)(produced by Part:BBa_I15008) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes. Associates with Part:BBa_I15010 to transduce a light signal into a genetic response.

6. Main Page (http://partsregistry.org/Part:BBa_K093005) - RFP with RBS, BBa_K093005. RFP, BBa_E1010, with Elowitz RBS, BBa_B0034.

7. Main page http://partsregistry.org/Part:BBa_R0011 - LacO – promoter, BBa_R0011: the promoter show the highest RPU-value of all characterized promoters and had the highest with IPTG.

8. Main page http://partsregistry.org/Part:BBa_K592026 J23101-B0032-BFP, Bba_K592026: this measurement construct show that constitutive promoter had 1.0 in RPU-value.

We´ve Also Characterized the Following Parts

Our measurement with the FACS AriaII shown the following Relative Promoter Units with the Blue Fluorescent Protein (mTagBFP) as reporter, assembled with B0032 in pSB3K3-backbone transformed in TOP10 E.coli-strain.

1. Main page http://partsregistry.org/Part:BBa_K592003 - cpcG2 promoter, BBa_K592003: measurement shows a RPU-value of 0,1.

2. Main page http://partsregistry.org/Part:BBa_K592006 - FixK2 promoter, BBa_K592006 is tightly regulated by the blue sensor and showed no significant expression in absence of the YF1-sensor (BBa_K592004)

3. Main page http://partsregistry.org/Part:BBa_K592008 - T5-lac Promoter, BBa_K592008: showed higher expression in the presence of IPTG than without it.