Team:Uppsala-Sweden/Project

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Ever since iGEM 2004, as more and more naturally occurring light-sensing microorganisms are being discovered and sequenced, synthetic biologists realize there is a whole range of natural light-sensing systems at their disposal. Other than using natural light-sensing proteins, engineered proteins have been designed from the natural templates. However, most of the light-sensing systems developed thus far focus on studying one light-sensing system at a time, characterizing the activation light spectra, active state, etc. Nobody has ever built one systems capable of detecting multiple wavelengths until [http://www.ncbi.nlm.nih.gov/pubmed/21035461 recently]. In short, our project focuses on improving the existing multichromatic sensing systems by expanding the number of useful wavelengths. Our system can regulate the expression of three different genes independently using three different wavelengths. These "multichromatic coliroids" upgrades the present coliroids, comparable to upgrading black-and-white movies to color TV. The proof of concept will be demonstrated by growing a colorful picture on bacteria culture, much like Andy Ellington's [http://partsregistry.org/Image:EllingtonColiroid.png coliroids picture] but with color.
Ever since iGEM 2004, as more and more naturally occurring light-sensing microorganisms are being discovered and sequenced, synthetic biologists realize there is a whole range of natural light-sensing systems at their disposal. Other than using natural light-sensing proteins, engineered proteins have been designed from the natural templates. However, most of the light-sensing systems developed thus far focus on studying one light-sensing system at a time, characterizing the activation light spectra, active state, etc. Nobody has ever built one systems capable of detecting multiple wavelengths until [http://www.ncbi.nlm.nih.gov/pubmed/21035461 recently]. In short, our project focuses on improving the existing multichromatic sensing systems by expanding the number of useful wavelengths. Our system can regulate the expression of three different genes independently using three different wavelengths. These "multichromatic coliroids" upgrades the present coliroids, comparable to upgrading black-and-white movies to color TV. The proof of concept will be demonstrated by growing a colorful picture on bacteria culture, much like Andy Ellington's [http://partsregistry.org/Image:EllingtonColiroid.png coliroids picture] but with color.
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== Main Project ==
 
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Regulation of gene expression by light is a milestone in synthetic biology. This rapidly developing field has attracted lots of attention in the recent years. Light regulation introduces noninvasive, direct and advanced spatiotemporal control of engineered biological systems. The aim of this project is a continuation of developing the above mentioned regulation method.
 
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In 2005, the world’s first light-sensing bacteria, “coliroids”, were engineered by scientists at UT Austin. Since then, as more and more naturally occurring light-sensing microorganisms are being discovered and sequenced, synthetic biologists realize there is a whole range of natural light-sensing systems at their disposal. Most of the light-sensing systems developed thus far focus on studying one light-sensing system at a time, characterizing its activation light spectra, active state, etc. There has been a lack of focus on building light-sensing systems that sense multiple wavelengths, until very recently. The ultimate objective is to introduce control of gene expression with multiple light wavelengths and demonstrate multidimensional light control as well as fine tunability of this system by making the engineered bacteria exhibit image based on three basic colors.
 

Revision as of 15:35, 13 July 2011

Uppsala University.



Project overview

Regulation of gene expression by light is a milestone in synthetic biology. As a contribution of UT Austin in iGEM 2004, the concept of "coliroids" was coined and thereby widely recognized. Light regulation introduces noninvasive, direct and advanced spatio-temporal control of engineered biological systems.

Ever since iGEM 2004, as more and more naturally occurring light-sensing microorganisms are being discovered and sequenced, synthetic biologists realize there is a whole range of natural light-sensing systems at their disposal. Other than using natural light-sensing proteins, engineered proteins have been designed from the natural templates. However, most of the light-sensing systems developed thus far focus on studying one light-sensing system at a time, characterizing the activation light spectra, active state, etc. Nobody has ever built one systems capable of detecting multiple wavelengths until recently. In short, our project focuses on improving the existing multichromatic sensing systems by expanding the number of useful wavelengths. Our system can regulate the expression of three different genes independently using three different wavelengths. These "multichromatic coliroids" upgrades the present coliroids, comparable to upgrading black-and-white movies to color TV. The proof of concept will be demonstrated by growing a colorful picture on bacteria culture, much like Andy Ellington's coliroids picture but with color.
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