Team:UTP-Panama/Modelling

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(The Project Design)
(PROJECT AND EXPERIMENTS DESIGN)
 
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==The Project Design==
 
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The Bristol BBa_k381001 encodes an expression of GFP in response of Nitrate or Nitrites that expresses fluorescent signals upon nutrient detection, its principal application is that allows farmers to quantify soil nutrient content.
 
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The BBa_k410000 is a fusion of the HydB cold shock promoter, the OmpA a signal peptide, and AOX 1 . AOXa is a alternative oxidase found in Sacred Lotus. This is going to produce a increase of heat above ambient temperature as follow:
 
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· From 20 °C to 30 ° C the HydB promoter will express.<br>
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<!--- The Mission, Experiments --->
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· Approximately at 37° the promoter will stop his expression.
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=='''PROJECT AND EXPERIMENTS DESIGN'''==
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__NOTOC__
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By combining  BBa_k38100 and BBa_k410000 we expected our part , the BBa_K672000 , to be able to sense nitrate and nitrites even after  experiencing a cold shock at 20º C, making our BB employable in other places where the temperatures can be as low as 15º C.
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<div align="center">
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'''In this section you can see the Experiments & Results with the Pre-existing Biobricks and the experiments with The RENBO'''
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[[File:BBCOMBINATIONreordered.jpg|750px]]
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This experiments and activities take a lot of time and EFFORT!!!
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'''By DNA ligation process we achieve our primary target, to join the two pre-existing BB and building a composite BB (BBa_k672000).'''</center>
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Note: We use '''E. Coli JM109''' for all our activities.
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==Experiments==
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<div align="center">
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[[File:experiment_1.jpg|250px|link=Team:UTP-Panama/Experiments]] [[File:experiment_2c.jpg|250px|link=Team:UTP-Panama/Experiments2]]  [[File:experiment_3.jpg|250px|link=Team:UTP-Panama/Experiments3]][[File:experiment4.jpg|250px|link=Team:UTP-Panama/Experiment4]]
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[[File:results_experiments.jpg|300px|link=Team:UTP-Panama/Results]]  [[File:newresults.jpg|300px|link=Team:UTP-Panama/NewResults]]

Latest revision as of 03:56, 29 October 2011

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PROJECT AND EXPERIMENTS DESIGN

In this section you can see the Experiments & Results with the Pre-existing Biobricks and the experiments with The RENBO


This experiments and activities take a lot of time and EFFORT!!!

Note: We use E. Coli JM109 for all our activities.


Experiment 1.jpg Experiment 2c.jpg Experiment 3.jpgExperiment4.jpg


Results experiments.jpg Newresults.jpg