http://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&feed=atom&action=historyTeam:UT-Tokyo/Data - Revision history2024-03-29T00:19:03ZRevision history for this page on the wikiMediaWiki 1.16.0http://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&diff=234635&oldid=prevM.shim at 02:11, 6 October 20112011-10-06T02:11:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Data for our favourite new parts=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Data for our favourite new parts=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518007 BBa_K518007] '''cheZ expression cassette (no promoter)'''<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518007 BBa_K518007] <ins class="diffchange diffchange-inline">''</ins>'''cheZ<ins class="diffchange diffchange-inline">'' </ins>expression cassette (no promoter)'''<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This construct rescues the mobility of cheZ<sup>-/-</sup> cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This construct rescues the mobility of cheZ<sup>-/-</sup> cells.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518010 BBa_K518010] '''sulA promoter'''<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518010 BBa_K518010] <ins class="diffchange diffchange-inline">''</ins>'''sulA<ins class="diffchange diffchange-inline">'' </ins>promoter'''<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This is a promoter of the sulA gene which is responsible for stress-induced arrest of cell division.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This is a promoter of the <ins class="diffchange diffchange-inline">''</ins>sulA<ins class="diffchange diffchange-inline">'' </ins>gene which is responsible for stress-induced arrest of cell division.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We successfully demonstrated a significant alteration of expression of a gene downstream of this promoter after UV irradiation. This <del class="diffchange diffchange-inline">promoter </del>can be utilized in our system.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We successfully demonstrated a significant alteration of expression of a gene downstream of this promoter after UV irradiation. This <ins class="diffchange diffchange-inline">part </ins>can be utilized <ins class="diffchange diffchange-inline">as a "signal induced promotor" </ins>in our system.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518013 BBa_K518013] '''sulA promoter evaluation device'''<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518013 BBa_K518013] <ins class="diffchange diffchange-inline">''</ins>'''sulA<ins class="diffchange diffchange-inline">'' </ins>promoter evaluation device'''<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>sulAp is known to respond to various types of DNA-injuring stress.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>sulAp is known to respond to various types of DNA-injuring stress.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This construct is a sulAp evaluation device to make it easy to compare the expression of sulAp in various "stressful" conditions.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This construct is a sulAp evaluation device to make it easy to compare the expression of sulAp in various "stressful" conditions.</div></td></tr>
</table>M.shimhttp://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&diff=234560&oldid=prevM.shim at 02:00, 6 October 20112011-10-06T02:00:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/BeginContent|fullpagename=Team:UT-Tokyo/Data|subpagename=Data}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/BeginContent|fullpagename=Team:UT-Tokyo/Data|subpagename=Data}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=How our system works=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=How our system works=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==SMART E.coli==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==SMART <ins class="diffchange diffchange-inline">''</ins>E. coli<ins class="diffchange diffchange-inline">''</ins>==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/Image|file=UT-Tokyo_Data-Top.png|caption=Figure 1. SMART E.coli}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/Image|file=UT-Tokyo_Data-Top.png|caption=Figure 1. SMART <ins class="diffchange diffchange-inline">''</ins>E. coli<ins class="diffchange diffchange-inline">''</ins>}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(A) Substrate-induced Cell Assembling System<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(A) Substrate-induced Cell Assembling System<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. Substrate (this time, an IPTG) induces expression from a substrate-induced promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011]).<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. Substrate (this time, an IPTG) induces expression from a substrate-induced promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011]).<br></div></td></tr>
</table>M.shimhttp://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&diff=234128&oldid=prevMarin21 at 01:14, 6 October 20112011-10-06T01:14:39Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. The cI-repressed promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 BBa_R0051]) is active when the cI repressor is absent.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. The cI-repressed promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 BBa_R0051]) is active when the cI repressor is absent.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5. CheZ is expressed and rescues the motility of cheZ<sup>-/-</sup> strain.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5. CheZ is expressed and rescues the motility of cheZ<sup>-/-</sup> strain.<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>6. When cI repressor ([http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]) is induced, it inhibits the activity of cI promotor. This results in the repression of CheZ expression, which leads to the loss of motility.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>6. When cI repressor ([http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]) is induced, it inhibits the activity of <ins class="diffchange diffchange-inline">''</ins>cI<ins class="diffchange diffchange-inline">'' </ins>promotor. This results in the repression of CheZ expression, which leads to the loss of motility.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518008 BBa_K518008] '''IPTG-inducible CheZ repression device'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518008 BBa_K518008] '''IPTG-inducible CheZ repression device'''<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This construct usually allows strong <del class="diffchange diffchange-inline">cheZ </del>expression, while in the presence of IPTG, the transcription of ''cheZ'' is repressed by the lambda ''cI'' repressor.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This construct usually allows strong <ins class="diffchange diffchange-inline">CheZ </ins>expression, while in the presence of IPTG, the transcription of ''cheZ'' is repressed by the lambda ''cI'' repressor.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518012 BBa_K518012] '''RBS + RFP + d.Ter'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518012 BBa_K518012] '''RBS + RFP + d.Ter'''<br></div></td></tr>
</table>Marin21http://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&diff=234104&oldid=prevMarin21 at 01:12, 6 October 20112011-10-06T01:12:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518008 BBa_K518008] '''IPTG-inducible CheZ repression device'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518008 BBa_K518008] '''IPTG-inducible CheZ repression device'''<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This construct usually allows strong cheZ expression, while in the presence of IPTG, the transcription of cheZ is repressed by the lambda cI repressor.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This construct usually allows strong cheZ expression, while in the presence of IPTG, the transcription of <ins class="diffchange diffchange-inline">''</ins>cheZ<ins class="diffchange diffchange-inline">'' </ins>is repressed by the lambda <ins class="diffchange diffchange-inline">''</ins>cI<ins class="diffchange diffchange-inline">'' </ins>repressor.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518012 BBa_K518012] '''RBS + RFP + d.Ter'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518012 BBa_K518012] '''RBS + RFP + d.Ter'''<br></div></td></tr>
</table>Marin21http://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&diff=233049&oldid=prevMasaya at 23:18, 5 October 20112011-10-05T23:18:12Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518010 BBa_K518010] '''sulA promoter'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518010 BBa_K518010] '''sulA promoter'''<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This is a promoter of the sulA gene which is responsible for stress-induced arrest of cell division.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This is a promoter of the sulA gene which is responsible for stress-induced arrest of cell division.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We successfully demonstrated a significant alteration of expression of a gene downstream of this promoter after UV irradiation</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We successfully demonstrated a significant alteration of expression of a gene downstream of this promoter after UV irradiation<ins class="diffchange diffchange-inline">. This promoter can be utilized in our system.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518013 BBa_K518013] '''sulA promoter evaluation device'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518013 BBa_K518013] '''sulA promoter evaluation device'''<br></div></td></tr>
</table>Masayahttp://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&diff=231112&oldid=prevHiro3726 at 20:17, 5 October 20112011-10-05T20:17:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Dual luciferase assay==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Dual luciferase assay==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/Image|file=UT-Tokyo_Data_luciferase.png|caption=Figure 2. Dual luciferase assay<del class="diffchange diffchange-inline">|desc=</del>}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/Image|file=UT-Tokyo_Data_luciferase.png|caption=Figure 2. Dual luciferase assay}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. A constitutive promoter of known strength (this time, [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23118 BBa_J23118]) is placed upstream of renilla luciferase expression cassette ([http://partsregistry.org/wiki/index.php?title=Part:BBa_BBa_K518001 BBa_K518001]), and a promoter of interest is placed upstream of firefly luciferase expression cassette ([http://partsregistry.org/wiki/index.php?title=Part:BBa_BBa_K518000 BBa_K518000]).<br> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. A constitutive promoter of known strength (this time, [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23118 BBa_J23118]) is placed upstream of renilla luciferase expression cassette ([http://partsregistry.org/wiki/index.php?title=Part:BBa_BBa_K518001 BBa_K518001]), and a promoter of interest is placed upstream of firefly luciferase expression cassette ([http://partsregistry.org/wiki/index.php?title=Part:BBa_BBa_K518000 BBa_K518000]).<br> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. After an instillation of first substrate D-luciferin, firefly luminescence is measured.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. After an instillation of first substrate D-luciferin, firefly luminescence is measured.<br></div></td></tr>
</table>Hiro3726http://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&diff=231107&oldid=prevHiro3726 at 20:17, 5 October 20112011-10-05T20:17:22Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Dual luciferase assay==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Dual luciferase assay==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/Image|file=UT-Tokyo_Data_luciferase.png|caption=Figure 2. Dual luciferase assay}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/Image|file=UT-Tokyo_Data_luciferase.png|caption=Figure 2. Dual luciferase assay<ins class="diffchange diffchange-inline">|desc=</ins>}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. A constitutive promoter of known strength (this time, [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23118 BBa_J23118]) is placed upstream of renilla luciferase expression cassette ([http://partsregistry.org/wiki/index.php?title=Part:BBa_BBa_K518001 BBa_K518001]), and a promoter of interest is placed upstream of firefly luciferase expression cassette ([http://partsregistry.org/wiki/index.php?title=Part:BBa_BBa_K518000 BBa_K518000]).<br> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. A constitutive promoter of known strength (this time, [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23118 BBa_J23118]) is placed upstream of renilla luciferase expression cassette ([http://partsregistry.org/wiki/index.php?title=Part:BBa_BBa_K518001 BBa_K518001]), and a promoter of interest is placed upstream of firefly luciferase expression cassette ([http://partsregistry.org/wiki/index.php?title=Part:BBa_BBa_K518000 BBa_K518000]).<br> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. After an instillation of first substrate D-luciferin, firefly luminescence is measured.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. After an instillation of first substrate D-luciferin, firefly luminescence is measured.<br></div></td></tr>
</table>Hiro3726http://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&diff=230243&oldid=prevHiro3726 at 19:15, 5 October 20112011-10-05T19:15:46Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:15, 5 October 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(B) Substrate-induced Cell Arrest System<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(B) Substrate-induced Cell Arrest System<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cheZ<sup>-/-</sup> strain is cloned for the works below. <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>cheZ<sup>-/-</sup> strain is cloned for the works below. <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>4. The <del class="diffchange diffchange-inline">c1</del>-repressed promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 BBa_R0051]) is active when the <del class="diffchange diffchange-inline">c1 </del>repressor is absent.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>4. The <ins class="diffchange diffchange-inline">cI</ins>-repressed promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 BBa_R0051]) is active when the <ins class="diffchange diffchange-inline">cI </ins>repressor is absent.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5. CheZ is expressed and rescues the motility of cheZ<sup>-/-</sup> strain.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5. CheZ is expressed and rescues the motility of cheZ<sup>-/-</sup> strain.<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>6. When <del class="diffchange diffchange-inline">c1 </del>repressor ([http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]) is induced, it inhibits the activity of <del class="diffchange diffchange-inline">c1 </del>promotor. This results in the repression of CheZ expression, which leads to the loss of motility.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>6. When <ins class="diffchange diffchange-inline">cI </ins>repressor ([http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]) is induced, it inhibits the activity of <ins class="diffchange diffchange-inline">cI </ins>promotor. This results in the repression of CheZ expression, which leads to the loss of motility.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518008 BBa_K518008] '''IPTG-inducible CheZ repression device'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518008 BBa_K518008] '''IPTG-inducible CheZ repression device'''<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This construct usually allows strong cheZ expression, while in the presence of IPTG, the transcription of cheZ is repressed by the lambda <del class="diffchange diffchange-inline">c1 </del>repressor.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This construct usually allows strong cheZ expression, while in the presence of IPTG, the transcription of cheZ is repressed by the lambda <ins class="diffchange diffchange-inline">cI </ins>repressor.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518012 BBa_K518012] '''RBS + RFP + d.Ter'''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K518012 BBa_K518012] '''RBS + RFP + d.Ter'''<br></div></td></tr>
</table>Hiro3726http://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&diff=230205&oldid=prevMarin21 at 19:13, 5 October 20112011-10-05T19:13:18Z<p></p>
<table style="background-color: white; color:black;">
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/Image|file=UT-Tokyo_Data-Top.png|caption=Figure 1. SMART E.coli}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/Image|file=UT-Tokyo_Data-Top.png|caption=Figure 1. SMART E.coli}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(A) Substrate-induced Cell Assembling System<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(A) Substrate-induced Cell Assembling System<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1. Substrate (this time, an IPTG) induces expression from a substrate-induced promoter (BBa_R0011).<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>1. Substrate (this time, an IPTG) induces expression from a substrate-induced promoter (<ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 </ins>BBa_R0011<ins class="diffchange diffchange-inline">]</ins>).<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>2. ''aspA'' (BBa_C0083) is expressed.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>2. ''aspA'' (<ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0083 </ins>BBa_C0083<ins class="diffchange diffchange-inline">]</ins>) is expressed.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3. AspA catalyses the production of L-aspartate from fumarate and ammonium ion.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3. AspA catalyses the production of L-aspartate from fumarate and ammonium ion.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(B) Substrate-induced Cell Arrest System<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(B) Substrate-induced Cell Arrest System<br></div></td></tr>
</table>Marin21http://2011.igem.org/wiki/index.php?title=Team:UT-Tokyo/Data&diff=230184&oldid=prevMarin21 at 19:11, 5 October 20112011-10-05T19:11:44Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:11, 5 October 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. The c1-repressed promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 BBa_R0051]) is active when the c1 repressor is absent.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. The c1-repressed promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 BBa_R0051]) is active when the c1 repressor is absent.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5. CheZ is expressed and rescues the motility of cheZ<sup>-/-</sup> strain.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5. CheZ is expressed and rescues the motility of cheZ<sup>-/-</sup> strain.<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>6. When c1 repressor (BBa_C0051) is induced, it inhibits the activity of c1 promotor. This results in the repression of CheZ expression, which leads to the loss of motility.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>6. When c1 repressor (<ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:</ins>BBa_C0051 <ins class="diffchange diffchange-inline">BBa_C0051]</ins>) is induced, it inhibits the activity of c1 promotor. This results in the repression of CheZ expression, which leads to the loss of motility.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Dual luciferase assay==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Dual luciferase assay==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/Image|file=UT-Tokyo_Data_luciferase.png|caption=Figure 2. Dual luciferase assay}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:UT-Tokyo/Templates/Image|file=UT-Tokyo_Data_luciferase.png|caption=Figure 2. Dual luciferase assay}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1. A constitutive promoter of known strength (this time, BBa_J23118) is placed upstream of renilla luciferase expression cassette (BBa_K518001), and a promoter of interest is placed upstream of firefly luciferase expression cassette (BBa_K518000).<br> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>1. A constitutive promoter of known strength (this time, <ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:</ins>BBa_J23118 <ins class="diffchange diffchange-inline">BBa_J23118]</ins>) is placed upstream of renilla luciferase expression cassette (<ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_BBa_K518001 </ins>BBa_K518001<ins class="diffchange diffchange-inline">]</ins>), and a promoter of interest is placed upstream of firefly luciferase expression cassette (<ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_BBa_K518000 </ins>BBa_K518000<ins class="diffchange diffchange-inline">]</ins>).<br> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. After an instillation of first substrate D-luciferin, firefly luminescence is measured.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. After an instillation of first substrate D-luciferin, firefly luminescence is measured.<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>3. After another substrate, coelenterazine, is added, renilla luminescence is measured as a reference to compute the ratio of the expression level of the target promoter to that of the internal control promoter (BBa_J23118).<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>3. After another substrate, coelenterazine, is added, renilla luminescence is measured as a reference to compute the ratio of the expression level of the target promoter to that of the internal control promoter (<ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:</ins>BBa_J23118 <ins class="diffchange diffchange-inline">BBa_J23118]</ins>).<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Data for our favourite new parts=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Data for our favourite new parts=</div></td></tr>
</table>Marin21