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Team:USTC-China/Project/results
From 2011.igem.org
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{{Team:USTC-China/temp/bar4}} | {{Team:USTC-China/temp/bar4}} | ||
<h1>Results</h1> | <h1>Results</h1> | ||
| - | ==Verification of ΔcheZ strain | + | ==Verification of ΔcheZ strain == |
<p>The size of colonies of RP1616 was much smaller than that of RP437 under same circumstances and after same period of incubation time. In colony PCR following, RP437 absolutely has much more outcome than RP1616 and we concluded that RP1616 is a ΔcheZ strain.</p> | <p>The size of colonies of RP1616 was much smaller than that of RP437 under same circumstances and after same period of incubation time. In colony PCR following, RP437 absolutely has much more outcome than RP1616 and we concluded that RP1616 is a ΔcheZ strain.</p> | ||
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==Verification of the function of the constructed riboswitch(protocol)== | ==Verification of the function of the constructed riboswitch(protocol)== | ||
0.25% Semisolid medium | 0.25% Semisolid medium | ||
Revision as of 18:42, 2 October 2011
Results
Verification of ΔcheZ strain
The size of colonies of RP1616 was much smaller than that of RP437 under same circumstances and after same period of incubation time. In colony PCR following, RP437 absolutely has much more outcome than RP1616 and we concluded that RP1616 is a ΔcheZ strain.
Verification of the function of the constructed riboswitch(protocol)
0.25% Semisolid medium
pp
From the results above we concluded that the constructed riboswitch functions well, especially on 0.3% Semisolid medium.
Verification of Toggle-switch (protocol)
Numbers of colonies with RFP: Numbers of colonies with GFP ≈ 8:25
Modulate Toggle-switches to produce more balanced ratio(protocol)
Numbers of colonies with RFP: Numbers of colonies with GFP ≈ 6:1
