Team:UST-Beijing

From 2011.igem.org

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     <td width="476"><h2 class="STYLE9">PROJECT</h2>
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     <p class="STYLE10"> Gene H-transfer: bile acid receptor in E.coli & proteorhodpsin in mitochindrial inner membrane
     <p class="STYLE10"> Gene H-transfer: bile acid receptor in E.coli & proteorhodpsin in mitochindrial inner membrane
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In order to celebrate the power of gene H(orizontal)-transfer between pro- and eukaryotes, we constructed two fusion proteins and tested their function: (1). a synthetic bile acid receptor in E.coli using a mammalian nuclear receptor LXR. As proof-of-principle, the regulatory circuit in symbiotic bacteria could be harmoniously linked to metabolic pathway of their host.  Potential application includes in situ synthesis of pharmaceuticals on-demand in the digestive tract.  (2) a synthetic light-driven proton pump in human mitocondrial inner membrane using a bacterial proteorhodopsin. Preliminary testing demonstrated cellular sensitivity to light radiation.  Application and utility relies on result of in-depth characterization of such system design.  For (1).We are using lacI DNA-binding domain and LXRbeta ligand-binding domain, we made  an artificial bile acid receptor which can regulate expression of target gene  within a natural lacI operon. As proof of principle, we demonstrate that  regulation of bacteria gene expression by host eukaryocyte metabolites is  achievable using chimeric nuclear receptors. Through directed molecular  evolution, a harmonious signal network regulating metabolism of both  prokaryocytes and their host eukaryocytes in the digestive tract is feasible. </p>
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In order to celebrate the power of gene H(orizontal)-transfer between pro- and eukaryotes, we constructed two fusion proteins and tested their function: (1). a synthetic bile acid receptor in E.coli using a mammalian nuclear receptor LXR. As proof-of-principle, the regulatory circuit in symbiotic bacteria could be harmoniously linked to metabolic pathway of their host.  Potential application includes in situ synthesis of pharmaceuticals on-demand in the digestive tract.  (2) a synthetic light-driven proton pump in human mitocondrial inner membrane using a bacterial proteorhodopsin. Preliminary testing demonstrated cellular sensitivity to light radiation.  Application and utility relies on result of in-depth characterization of such system design.   
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(1).We are using lacI DNA-binding domain and LXRbeta ligand-binding domain, we made  an artificial bile acid receptor which can regulate expression of target gene  within a natural lacI operon. As proof of principle, we demonstrate that  regulation of bacteria gene expression by host eukaryocyte metabolites is  achievable using chimeric nuclear receptors. Through directed molecular  evolution, a harmonious signal network regulating metabolism of both  prokaryocytes and their host eukaryocytes in the digestive tract is feasible. </p>
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     <p class="STYLE10"><img src="https://static.igem.org/mediawiki/2011/5/5a/LacI_rUR.png" width="233" height="222" /><img src="https://static.igem.org/mediawiki/2011/5/5a/Bile_%EF%BC%A1%EF%BD%83%EF%BD%89%EF%BD%84.jpg.jpg" width="233" height="222" /></p>
     <p class="STYLE10"><img src="https://static.igem.org/mediawiki/2011/5/5a/LacI_rUR.png" width="233" height="222" /><img src="https://static.igem.org/mediawiki/2011/5/5a/Bile_%EF%BC%A1%EF%BD%83%EF%BD%89%EF%BD%84.jpg.jpg" width="233" height="222" /></p>
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Revision as of 05:22, 2 September 2011

Home Page

 

 

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UST-Beijing

 

 
 
 

 
 
Welcome to UST-Beijing

 


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Instructors

Prof.Ching Song

Doc. Huai Zhang

 

PROJECT

Gene H-transfer: bile acid receptor in E.coli & proteorhodpsin in mitochindrial inner membrane . In order to celebrate the power of gene H(orizontal)-transfer between pro- and eukaryotes, we constructed two fusion proteins and tested their function: (1). a synthetic bile acid receptor in E.coli using a mammalian nuclear receptor LXR. As proof-of-principle, the regulatory circuit in symbiotic bacteria could be harmoniously linked to metabolic pathway of their host. Potential application includes in situ synthesis of pharmaceuticals on-demand in the digestive tract. (2) a synthetic light-driven proton pump in human mitocondrial inner membrane using a bacterial proteorhodopsin. Preliminary testing demonstrated cellular sensitivity to light radiation. Application and utility relies on result of in-depth characterization of such system design. For (1).We are using lacI DNA-binding domain and LXRbeta ligand-binding domain, we made an artificial bile acid receptor which can regulate expression of target gene within a natural lacI operon. As proof of principle, we demonstrate that regulation of bacteria gene expression by host eukaryocyte metabolites is achievable using chimeric nuclear receptors. Through directed molecular evolution, a harmonious signal network regulating metabolism of both prokaryocytes and their host eukaryocytes in the digestive tract is feasible.

 

 

 

 

Parts Submitted to the Registry

 

 
Acknowledgement

TEAM

These are our team members for the 2011 iGEM, Do you want to know more about us ? ;click here

sponsors

Safety

We had a serious discussion before and we asked ourselves the following four questions. And of course, We got the answers.

1.Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety? Since modern time molecular biology and gene engineering is a "reverse engineering" process, which means that we discover and acquire research tools from the existing genes and structures, our iGEM projects in theory might influence natural and existing biological structures and functions in our laboratory.However, any reagents made in our lab is not infectious or contiguous; even in the case of being released by accident, will not impose safety threats to our lab members, people around us, general public, or environment in general. Early pioneers of molecular biology developed a self-disciplined code of ethics to prevent intentional usage of biological reagents, and engineered a stack of laboratory-dependent species for research-only use to prevent unintentional release and contamination of the environment. We plan to inherit the high standard ethical code of action and common molecular biology reagents to meet the requirement for human and environmental safety. In addition, we will introduce biosafety issues and guide-lines to the undergraduate students of life science major as an inseparable part of the academic curriculum to advocate biosafety awareness and promote biosafety education. Read more

Notebook