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Team:UPO-Sevilla/Foundational Advances/MiniTn7/Data Page
From 2011.igem.org
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| - | < | + | <h4>Data for our characterized new parts</h4> |
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<li><p><strong>pUC18Sfi-miniTn7BB-Gm plasmid</strong> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K510000" target="_blank">BBa_K510000</a>): this plasmid harbor the basic miniTn7 which may be used for integration of BioBricks in single copy into the genomes of non-enteric bacteria, in which it is non-replicative, at the conserved attTn7 site. | <li><p><strong>pUC18Sfi-miniTn7BB-Gm plasmid</strong> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K510000" target="_blank">BBa_K510000</a>): this plasmid harbor the basic miniTn7 which may be used for integration of BioBricks in single copy into the genomes of non-enteric bacteria, in which it is non-replicative, at the conserved attTn7 site. | ||
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<li><p><strong>attTn7 or Tn7 insertion site</strong> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K510022" target="_blank">BBa_K510022</a>): recognition and insertion site of the Tn7 transposon and its synthetic derivatives. | <li><p><strong>attTn7 or Tn7 insertion site</strong> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K510022" target="_blank">BBa_K510022</a>): recognition and insertion site of the Tn7 transposon and its synthetic derivatives. | ||
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</p></li> | </p></li> | ||
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</ol> | </ol> | ||
| + | </br> | ||
| + | <h4>We have also submetted this new parts:</h4> | ||
| + | <ol> | ||
| + | <li><p><strong>pUC18SfiI-miniTn7BB-Cm</strong> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K510001" target="_blank">BBa_K510001</a>): this construct is the result of replacing the gentamycin resistance cassette in pUC18SfiI-miniTn7BB-Gm by a chloramphenicol resistance cassette obtained from pBS1C3 by PCR amplification. | ||
| + | </p></li> | ||
| + | <li><p><strong>pUC18SfiI-miniTn7BB-Km</strong> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K510002" target="_blank">BBa_K510002</a>): replace of the gentamycin resistance cassette of pUC18Sfi-miniTn7-Gm by a kanamycin resistance cassette amplified from pSB4K5. | ||
| + | </p></li> | ||
| + | <li><p><strong>pUC18R6KT-miniTn7BB-Cm</strong> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K510013" target="_blank">BBa_K510013</a>): replace of the gentamycin resistance cassette in pUC18R6KT-miniTn7BB-Gm by a chloramphenicol resistance cassette obtained from pBS1C3 by PCR amplification with these primers | ||
| + | </p></li> | ||
| + | <li><p><strong>pUC18R6KT-miniTn7BB-Km</strong> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K510014" target="_blank">BBa_K510014</a>): replace of the gentamycin resistance cassette in pUC18R6KT-miniTn7BB-Gm by a kanamycin resistance cassette obtained from pBS4K5. | ||
| + | </p></li> | ||
| + | <li><p><strong></strong> (<a href="" target="_blank"></a>): | ||
| + | </p></li> | ||
| + | <li><p><strong></strong> (<a href="" target="_blank"></a>): | ||
| + | </p></li> | ||
| + | </ol> | ||
Revision as of 16:59, 20 September 2011
Data Page
Data for our characterized new parts
pUC18Sfi-miniTn7BB-Gm plasmid (BBa_K510000): this plasmid harbor the basic miniTn7 which may be used for integration of BioBricks in single copy into the genomes of non-enteric bacteria, in which it is non-replicative, at the conserved attTn7 site.
pUC18R6KT-miniTn7BB-Gm plasmid (BBa_K510012): this plasmid may be used for transposition of BioBricks into the genome of any bacterium without the pir gen thanks to is R6K replication origin.
attTn7 or Tn7 insertion site (BBa_K510022): recognition and insertion site of the Tn7 transposon and its synthetic derivatives.
We have also submetted this new parts:
pUC18SfiI-miniTn7BB-Cm (BBa_K510001): this construct is the result of replacing the gentamycin resistance cassette in pUC18SfiI-miniTn7BB-Gm by a chloramphenicol resistance cassette obtained from pBS1C3 by PCR amplification.
pUC18SfiI-miniTn7BB-Km (BBa_K510002): replace of the gentamycin resistance cassette of pUC18Sfi-miniTn7-Gm by a kanamycin resistance cassette amplified from pSB4K5.
pUC18R6KT-miniTn7BB-Cm (BBa_K510013): replace of the gentamycin resistance cassette in pUC18R6KT-miniTn7BB-Gm by a chloramphenicol resistance cassette obtained from pBS1C3 by PCR amplification with these primers
pUC18R6KT-miniTn7BB-Km (BBa_K510014): replace of the gentamycin resistance cassette in pUC18R6KT-miniTn7BB-Gm by a kanamycin resistance cassette obtained from pBS4K5.
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