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Team:UNIST Korea/project/modules
From 2011.igem.org
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<th>Processing Module</th><th>Lysis Module</th> | <th>Processing Module</th><th>Lysis Module</th> | ||
</tr></table><br/><br/> | </tr></table><br/><br/> | ||
| - | <b><font size=" | + | <b><font size="4"><font color="blue">SENSORY MODULE</b></font></font><br/><br/> |
| - | We have engineered two different sensors into <i>E. coli</i>. The first sensor is an optical sensor that detects the light present in the environment. We used Cph8, hybrid light receptor, as a optical sensor (Fig 1) [1]. The second sensor is to a physical sensor that would sense the temperature of the environment. We used the temperature dependent ribo-switch as a physical sensor to detect the temperature of the environment (Fig 2) [2].<br/><br/> | + | We have engineered two different sensors into <i>E. coli</i>. The first sensor is an <font color="blue"><b>optical sensor</b></font> that detects the light present in the environment. We used Cph8, hybrid light receptor, as a optical sensor (Fig 1) [1]. The second sensor is to a <font color="blue"><b>physical sensor</b></font> that would sense the temperature of the environment. We used the temperature dependent ribo-switch as a physical sensor to detect the temperature of the environment (Fig 2) [2].<br/><br/> |
| - | <center> <img src="https://static.igem.org/mediawiki/ | + | <center> <img src="https://static.igem.org/mediawiki/2011/1/1b/Picture1.png" width="400" height="300"/></center> |
<b>Figure 1 – Efficiency of the optical sensor was evaluated by following GFP expression in the presence and absence of light.</b> | <b>Figure 1 – Efficiency of the optical sensor was evaluated by following GFP expression in the presence and absence of light.</b> | ||
<center> <img src="https://static.igem.org/mediawiki/2011/c/c8/Picture2.jpg" width="700" height="300"/></center><br/> | <center> <img src="https://static.igem.org/mediawiki/2011/c/c8/Picture2.jpg" width="700" height="300"/></center><br/> | ||
| - | <b>Figure 2: The physical sensor engineered in <i>Chop. coli</i> helps it to differentiate between 30⁰C and 37⁰C when GFP was fused with the temperature dependent ribo-switch. </b><br/><br/> | + | <b>Figure 2: The physical sensor engineered in <i>Chop. coli</i> helps it to differentiate between 30⁰C and 37⁰C when GFP was fused with the temperature dependent ribo-switch. </b><br/> |
| - | <center><b><font size=" | + | |
| + | Being aware that fermentor is always maintained at 37⁰C, we assumed that the physical sensor would help <i>Chop. coli</i> to differentiate its environment. However, one cannot guarantee the presence of complete darkness in the fermentor. We hypothesized if the hybrid light receptor (hybrid of light receptor and osmo regulator) could also help determine the osmolarity. The fermentor is supposed to contain sugar at a higher concentration than the environment. As expected, the light receptor could sense high sugar concentration and behave similar to darkness in the presence of high sugar concentration. This would be an advantageous feature in <i>Chop. coli</i>. | ||
| + | <center> <img src="https://static.igem.org/mediawiki/2011/2/2c/SKL12.png" width="800" height="300"/></center><br/><br/> | ||
| + | <center><b><font size="5"><font color="blue"><i>Chop. coli</i> can efficiently sense its environment</center></b></font></font><br/> | ||
<table style="background-color:#F5F5DC;"><table width="100%" border="0"> | <table style="background-color:#F5F5DC;"><table width="100%" border="0"> | ||
<tr> | <tr> | ||
| - | <th><b><font size=" | + | <th><b><font size="4"><font color="red">Temperature</font></font></th> |
<th><font size="5"><font color="red">Light</font></font></th> | <th><font size="5"><font color="red">Light</font></font></th> | ||
<th><font size="5"><font color="red">Osmolality</font></font></th> | <th><font size="5"><font color="red">Osmolality</font></font></th> | ||
</tr></table><br/><br/> | </tr></table><br/><br/> | ||
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</body> | </body> | ||
</HTML> | </HTML> | ||
Revision as of 11:10, 4 October 2011
RESULTS
Our project aims at designing E. coli to sense its environment and act accordingly. In order to achieve this, we have categorized our projects into three main groups.
| Sensory Module | Processing Module | Lysis Module |
|---|
SENSORY MODULE
We have engineered two different sensors into E. coli. The first sensor is an optical sensor that detects the light present in the environment. We used Cph8, hybrid light receptor, as a optical sensor (Fig 1) [1]. The second sensor is to a physical sensor that would sense the temperature of the environment. We used the temperature dependent ribo-switch as a physical sensor to detect the temperature of the environment (Fig 2) [2].
Figure 2: The physical sensor engineered in Chop. coli helps it to differentiate between 30⁰C and 37⁰C when GFP was fused with the temperature dependent ribo-switch.
Being aware that fermentor is always maintained at 37⁰C, we assumed that the physical sensor would help Chop. coli to differentiate its environment. However, one cannot guarantee the presence of complete darkness in the fermentor. We hypothesized if the hybrid light receptor (hybrid of light receptor and osmo regulator) could also help determine the osmolarity. The fermentor is supposed to contain sugar at a higher concentration than the environment. As expected, the light receptor could sense high sugar concentration and behave similar to darkness in the presence of high sugar concentration. This would be an advantageous feature in Chop. coli.
| Temperature | Light | Osmolality |
|---|
