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Team:UNIST Korea/project/modules
From 2011.igem.org
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<font size="6"><font color="blue">RESULTS</font></font><br/><br/> | <font size="6"><font color="blue">RESULTS</font></font><br/><br/> | ||
| - | Our project aims at designing <i>E. coli</i> to sense its environment and act accordingly. In order to achieve this, we have categorized our projects into three main groups.<br/> | + | Our project aims at designing <i>E. coli</i> to sense its environment and act accordingly. In order to achieve this, we have categorized our projects into three main groups.<br/> <br/> |
| - | <img src="https://static.igem.org/mediawiki/2011/2/2e/Siganl_module.png" width=" | + | <img src="https://static.igem.org/mediawiki/2011/2/2e/Siganl_module.png" width="275" height="300"> |
| - | <img src="https://static.igem.org/mediawiki/2011/8/8c/Process_module.png" width=" | + | <img src="https://static.igem.org/mediawiki/2011/8/8c/Process_module.png" width="275" height="300"> |
| - | <img src="https://static.igem.org/mediawiki/2011/6/6b/Lysis_module.png" width=" | + | <img src="https://static.igem.org/mediawiki/2011/6/6b/Lysis_module.png" width="275" height="300"> |
| - | </img><br/>< | + | </img> |
| - | <br> | + | <br/><table style="background-color:#F5F5DC;"><table width="75%" border="0"> |
| + | |||
| + | |||
| + | <tr> | ||
| + | <th><b>Sensory Module</th> | ||
| + | <th>Processing Module</th><th>Lysis Module</th> | ||
| + | </tr></table><br/><br/> | ||
<b><font size="6"><font color="blue">SENSORY MODULE</b></font></font><br/><br/> | <b><font size="6"><font color="blue">SENSORY MODULE</b></font></font><br/><br/> | ||
We have engineered two different sensors into <i>E. coli</i>. The first sensor is an optical sensor that detects the light present in the environment. We used Cph8, hybrid light receptor, as a optical sensor (Fig 1) [1]. The second sensor is to a physical sensor that would sense the temperature of the environment. We used the temperature dependent ribo-switch as a physical sensor to detect the temperature of the environment (Fig 2) [2].<br/><br/> | We have engineered two different sensors into <i>E. coli</i>. The first sensor is an optical sensor that detects the light present in the environment. We used Cph8, hybrid light receptor, as a optical sensor (Fig 1) [1]. The second sensor is to a physical sensor that would sense the temperature of the environment. We used the temperature dependent ribo-switch as a physical sensor to detect the temperature of the environment (Fig 2) [2].<br/><br/> | ||
| - | <center> <img src="https://static.igem.org/mediawiki/igem.org/1/1b/Picture1.png" width=" | + | <center> <img src="https://static.igem.org/mediawiki/igem.org/1/1b/Picture1.png" width="400" height="300"/></center> |
| - | + | <b>Figure 1 – Efficiency of the optical sensor was evaluated by following GFP expression in the presence and absence of light.</b> | |
| + | <center> <img src="https://static.igem.org/mediawiki/2011/c/c8/Picture2.jpg" width="700" height="300"/></center><br/> | ||
| + | <b>Figure 2: The physical sensor engineered in <i>Chop. coli</i> helps it to differentiate between 30⁰C and 37⁰C when GFP was fused with the temperature dependent ribo-switch. </b><br/><br/> | ||
| + | <center><b><font size="6"><font color="blue"><i>Chop. coli</i> can efficiently sense its environment</center></b></font></font><br/> | ||
| + | <table style="background-color:#F5F5DC;"><table width="100%" border="0"> | ||
| + | <tr> | ||
| + | <th><b><font size="5"><font color="red">Temperature</font></font></th> | ||
| + | <th><font size="5"><font color="red">Light</font></font></th> | ||
| + | <th><font size="5"><font color="red">Osmolality</font></font></th> | ||
| + | </tr></table><br/><br/> | ||
| + | Being aware that fermentor is always maintained at 37⁰C, we assumed that the physical sensor would help <i>Chop. coli</i> to differentiate its environment. However, one cannot guarantee the presence of complete darkness in the fermentor. We hypothesized if the hybrid light receptor (hybrid of light receptor and osmo regulator) could also help determine the osmolarity. The fermentor is supposed to contain sugar at a higher concentration than the environment. As expected, the light receptor could sense high sugar concentration and behave similar to darkness in the presence of high sugar concentration. This would be an advantageous feature in <i>Chop. coli</i>. | ||
| + | <center> <img src="https://static.igem.org/mediawiki/2011/2/2c/SKL12.png" width="800" height="300"/></center><br/> | ||
</body> | </body> | ||
</HTML> | </HTML> | ||
Revision as of 11:05, 4 October 2011
RESULTS
Our project aims at designing E. coli to sense its environment and act accordingly. In order to achieve this, we have categorized our projects into three main groups.
| Sensory Module | Processing Module | Lysis Module |
|---|
SENSORY MODULE
We have engineered two different sensors into E. coli. The first sensor is an optical sensor that detects the light present in the environment. We used Cph8, hybrid light receptor, as a optical sensor (Fig 1) [1]. The second sensor is to a physical sensor that would sense the temperature of the environment. We used the temperature dependent ribo-switch as a physical sensor to detect the temperature of the environment (Fig 2) [2].
Figure 2: The physical sensor engineered in Chop. coli helps it to differentiate between 30⁰C and 37⁰C when GFP was fused with the temperature dependent ribo-switch.
| Temperature | Light | Osmolality |
|---|
Being aware that fermentor is always maintained at 37⁰C, we assumed that the physical sensor would help Chop. coli to differentiate its environment. However, one cannot guarantee the presence of complete darkness in the fermentor. We hypothesized if the hybrid light receptor (hybrid of light receptor and osmo regulator) could also help determine the osmolarity. The fermentor is supposed to contain sugar at a higher concentration than the environment. As expected, the light receptor could sense high sugar concentration and behave similar to darkness in the presence of high sugar concentration. This would be an advantageous feature in Chop. coli.
