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Team:UC Davis/Project
From 2011.igem.org
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<h2>Promoter Mutants</h2> | <h2>Promoter Mutants</h2> | ||
| - | We chose repressible promoters because they are useful in designing complex circuits and are relatively easy to screen for changes in activity level. You can view detailed information on our <a href="https://2011.igem.org/Team:UC_Davis/LacI">LacI</a>, <a href="https://2011.igem.org/Team:UC_Davis/TetR">TetR</a> and <a href="https://2011.igem.org/Team:UC_Davis/ | + | We chose to mutate repressible promoters because they are useful in designing complex circuits and are relatively easy to screen for changes in activity level. You can view detailed information on our <a href="https://2011.igem.org/Team:UC_Davis/PromoterFamilies#LacI">LacI</a>, <a href="https://2011.igem.org/Team:UC_Davis/PromoterFamilies#TetR">TetR</a> and <a href="https://2011.igem.org/Team:UC_Davis/PromoterFamilies#c1">λ cI</a> part families on their respective pages.<br><br> |
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Revision as of 17:34, 22 October 2011
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View our judging criteria for iGEM 2011 here.
Overview
We set out to develop a rugged process for the rapid production of mutant libraries of any BioBrick part using standard primers and a simple mutagenic PCR protocol. We chose to prototype this process by creating mutant libraries of the LacI, TetR and λ cI repressible promoters and to mutate GFP to visually assess our ability to create functional protein mutants.
As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI promoter mutants. We also have nine TetR promoter mutants, seven λ cI promoter mutants, and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.
As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI promoter mutants. We also have nine TetR promoter mutants, seven λ cI promoter mutants, and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.
