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Team:UC Davis/Notebook/Week 15
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Revision as of 07:33, 28 September 2011
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Week 15 description.
Week 15
--Monday 9/19/11--
After the calibration run that we did late last week, we think we know what the actual values for our old arabinose stock were. Unfortunately, where our data started to line up was at the very end of the serial dilution, so we think our new stocks need to be diluted more. We're going to do one more calibration run just to make sure that we really have calibrated our data. We can't afford to make assumptions that aren't true at this point! Until we confirm this, we'll keep characterizing LacI mutants with the old uncalibrated stock.
Good news at the end of the day, we've finally figured out what our old arabinose concentration really was!
| Old/Wrong Arabinose Concentration by Weight | Actual Arabinose Concentration by Weight |
| 0% | 0% |
| 0.2% | 0.0014% |
| 0.4% | 0.0028% |
| 0.6% | 0.0042% |
| 0.8% | 0.0056% |
| 1% | 0.0070% |
| 2% | 0.0140% |
--Tuesday 9/20/11--
Now that we have the the real values for our range of arabinose, we can continue to screen our promoter mutants with our new stocks. Today we're going to do a characterization run for LacI mutant #6 (K611026) and start actually characterizing our R0051 and R0040 mutants. We'll start off with R0040 #1, #8, and #9 at the same arabinose levels with which we've been characterizing LacI.
--Wednesday 9/21/11--
We're continuing with our characterization today by doing a run with some of our R0051 mutants (#1, #4, and #5). It looks like the rest of the Tecan runs up to the deadline on the 28th will be characterization runs. We still need to characterize LacI mutant #7 (K611027), pTet #2-#7, and cI lambda #2, #3, and #6-9.
--Thursday 9/22/11--
Characterization is continuing as planned. Today we'll characterize R0051 #2, #6, #7, and #8, and R0040 #2, #5, #6, and #7.
--Friday 9/23/11--
--Saturday 9/24/11--
