Team:UC Davis/Data LambdacI

From 2011.igem.org

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<h1 id="widget"> λ cI Promoter Characterization Data</h1>
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<h1>λ c1 Promoter Data</h1>
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<div id="mutantwidget" class="floatbox2">
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<h3>Wildtype</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.99566, 0.95671, 0.880328, 0.734557, 0.662629, 0.607491, 0.530352</span>
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<span class="stdevs"> 0.012905, 0.015917, 0.020404, 0.026943, 0.013849, 0.0068952, 0.0060456</span>
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<span class="numconditions">4</span>
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<h3>Mutant 1</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.6492, 0.20526, 0.17329, 0.16899, 0.16682, 0.16631, 0.16913</span>
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<span class="stdevs"> 0.017682, 0.0032754, 0.0010138, 0.0011266, 0.00097033, 0.0012072, 0.0011179</span>
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<h3>Mutant 2</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.53303, 0.16064, 0.14171, 0.12149, 0.12547, 0.11146, 0.1061</span>
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<span class="stdevs"> 0.02651, 0.0029121, 0.0027198, 0.0033112, 0.0034421, 0.0059837, 0.0027095</span>
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<h3>Mutant 4</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.23, 0.038825, 0.035663, 0.036119, 0.035423, 0.034289, 0.032858</span>
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<span class="stdevs"> 0.02048, 0.00047265, 0.00028731, 0.00037973, 0.00031652, 0.00026622, 0.00070058</span>
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<h3>Mutant 5</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.92311, 0.23346, 0.1833, 0.17092, 0.16534, 0.16473, 0.15538</span>
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<span class="stdevs"> 0.0061434, 0.0029621, 0.0016015, 0.0037633, 0.0029015, 0.002096, 0.0024785</span>
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<h3>Mutant 6</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.97182, 0.98592, 1.01000, 1.02300, 1.03610, 1.04490, 1.01750</span>
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<span class="stdevs"> 0.018621, 0.01966, 0.018634, 0.018826, 0.018993, 0.020762, 0.019842</span>
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<h3>Mutant 7</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.48325, 0.13919, 0.12353, 0.10617, 0.10943, 0.099213, 0.08565</span>
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<span class="stdevs"> 0.012073, 0.0025601, 0.0030506, 0.0027667, 0.002179, 0.0022998, 0.0015159</span>
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<h3>Mutant 8</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.41657, 0.12325, 0.10744, 0.10365, 0.10006, 0.096006, 0.09219</span>
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<span class="stdevs"> 0.0080566, 0.0028055, 0.0027614, 0.0038199, 0.0038188, 0.0024541, 0.0022523</span>
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The above widget displays the relative GFP fluorescence data that we have collected for the 9 lambda cI mutant promoters and the wild type R0051 promoter. The plots are normalized to the wild type such that the wild type's fluorescence at 0% arabinose and 0 mM IPTG is 1. The data for these plots were gathered using the constructs outlined on the <a href="https://2011.igem.org/Team:UC_Davis/cilambda>lambda cI project page</a>, which were designed so that increasing the arabinose concentration would proportionally increase the repressor concentration.
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We are still in the process of screening λ c1 promoters for GFP activity and do not have final data to to present at this time.<br><br>
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Check back here for updates after the American regional comepetition!
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Latest revision as of 01:50, 29 September 2011

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Criteria

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λ c1 Promoter Data

We are still in the process of screening λ c1 promoters for GFP activity and do not have final data to to present at this time.

Check back here for updates after the American regional comepetition!

Retrieved from "http://2011.igem.org/Team:UC_Davis/Data_LambdacI"