Team:UC Davis/Data LambdacI

From 2011.igem.org

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<div class="floatbox">
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<h1 id="widget">LacI Promoter Characterization Data</h1>
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<h1>λ c1 Promoter Data</h1>
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<div id="mutantwidget" class="floatbox2">
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<div class="floatbox3">
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<h3>Wildtype</h3>
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We are still in the process of screening λ c1 promoters for GFP activity and do not have final data to to present at this time.<br><br>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
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0.99315,
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  0.78019, 
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  0.58405,
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  0.45400, 
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  0.35401,
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  0.29099, 
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  0.13305, 
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</span>
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<span class="numconditions">4</span>
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<--!<span class='xdata_3d'>0, 1, 2, 5</span>
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<span class='ydata_3d'>0.00000,  0.20000,  0.40000,  0.60000,  0.80000,  1.00000,  2.00000</span>
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<span class='zdata_3d'>
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  0.99315,  1.02097,  0.99023,  0.99354,
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  0.78019,  0.97021,  0.96725,  0.96226,
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  0.58405,  0.87100,  0.89795,  0.94529,
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  0.45400,  0.76333,  0.85071,  0.93618,
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  0.35401,  0.65243,  0.75444,  0.88056,
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  0.29099,  0.55454,  0.68266,  0.84441,
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  0.13305,  0.26829,  0.37015,  0.55294</span>--!>
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<h3>Mutant 1</h3>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
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  0.68829,
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  0.48655, 
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  0.27198,
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  0.15260, 
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  0.08426,
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  0.05322, 
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  0.01741, 
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</span>
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<h3>Mutant 2</h3>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
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  0.68514,
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  0.43727, 
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  0.27724,
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  0.17817, 
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  0.11684,
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  0.08021, 
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  0.03415, 
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</span>
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<h3>Mutant 3</h3>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
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  0.67026,
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  0.44322, 
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  0.28504,
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  0.17543, 
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  0.10938,
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  0.07325, 
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  0.02937, 
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</span>
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<h3>Mutant 4</h3>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
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  0.31888,
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  0.16625, 
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  0.10228,
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  0.05821, 
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  0.03985,
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  0.02549, 
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  0.01329, 
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</span>
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<h3>Mutant 5</h3>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
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  0.63174,
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  0.33524, 
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  0.17440,
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  0.09337, 
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  0.03043,
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  0.03337, 
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  0.01686, 
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</span>
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<h3>Mutant 6</h3>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
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  0.42290,
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  0.29347, 
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  0.15733,
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  0.09471, 
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  0.05778,
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  0.03850, 
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  0.01475, 
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</span>
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<h3>Mutant 7</h3>
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<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata">
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  0.58989,
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  0.32402, 
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  0.20047,
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  0.11183, 
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  0.06365,
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  0.03915, 
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  0.01712, 
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</span>
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</div>
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</div>
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<div class="floatbox">
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Check back here for updates after the American regional comepetition!
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<center>
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<div class="floatbox3">
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<img src="https://static.igem.org/mediawiki/2011/d/d9/UCD_Mut_graph_lacI.png" width="680" height="350">
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Some preliminary data.  These are LacI promoter mutants.  The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter.  There's still more to come!
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</center>
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Latest revision as of 01:50, 29 September 2011

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λ c1 Promoter Data

We are still in the process of screening λ c1 promoters for GFP activity and do not have final data to to present at this time.

Check back here for updates after the American regional comepetition!

Retrieved from "http://2011.igem.org/Team:UC_Davis/Data_LambdacI"