Team:UC Davis/Data LambdacI

From 2011.igem.org

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The above widget displays the relative GFP fluorescence data that we have collected for the 9 lambda cI mutant and the wild type R0051 promoter promoters. The plots are normalized to the wild type such that the wild type's fluorescence at 0% arabinose and 0 mM IPTG is 1. The data for these plots were gathered using the constructs on the <a href="https://2011.igem.org/Team:UC_Davis/cilambda>lambda cI project page</a>.
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Some preliminary data.  These are LacI promoter mutants.  The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter.  There's still more to come!
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Revision as of 09:23, 28 September 2011

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λ cI Promoter Characterization Data

Wildtype

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.99566, 0.95671, 0.880328, 0.734557, 0.662629, 0.607491, 0.530352 0.012905, 0.015917, 0.020404, 0.026943, 0.013849, 0.0068952, 0.0060456 4

Mutant 1

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.6492, 0.20526, 0.17329, 0.16899, 0.16682, 0.16631, 0.16913 0.017682, 0.0032754, 0.0010138, 0.0011266, 0.00097033, 0.0012072, 0.0011179

Mutant 2

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.53303, 0.16064, 0.14171, 0.12149, 0.12547, 0.11146, 0.1061 0.02651, 0.0029121, 0.0027198, 0.0033112, 0.0034421, 0.0059837, 0.0027095

Mutant 4

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.23, 0.038825, 0.035663, 0.036119, 0.035423, 0.034289, 0.032858 0.02048, 0.00047265, 0.00028731, 0.00037973, 0.00031652, 0.00026622, 0.00070058

Mutant 5

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.92311, 0.23346, 0.1833, 0.17092, 0.16534, 0.16473, 0.15538 0.0061434, 0.0029621, 0.0016015, 0.0037633, 0.0029015, 0.002096, 0.0024785

Mutant 6

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.97182, 0.98592, 1.01000, 1.02300, 1.03610, 1.04490, 1.01750 0.018621, 0.01966, 0.018634, 0.018826, 0.018993, 0.020762, 0.019842

Mutant 7

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.48325, 0.13919, 0.12353, 0.10617, 0.10943, 0.099213, 0.08565 0.012073, 0.0025601, 0.0030506, 0.0027667, 0.002179, 0.0022998, 0.0015159

Mutant 8

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.41657, 0.12325, 0.10744, 0.10365, 0.10006, 0.096006, 0.09219 0.0080566, 0.0028055, 0.0027614, 0.0038199, 0.0038188, 0.0024541, 0.0022523
The above widget displays the relative GFP fluorescence data that we have collected for the 9 lambda cI mutant and the wild type R0051 promoter promoters. The plots are normalized to the wild type such that the wild type's fluorescence at 0% arabinose and 0 mM IPTG is 1. The data for these plots were gathered using the constructs on the Retrieved from "http://2011.igem.org/Team:UC_Davis/Data_LambdacI"