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| - | {{:Team:UANL_Mty-Mexico/Templates/Banner-template}}
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| - | {{:Team:UANL_Mty-Mexico/Templates/Menu-template}}
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| - | <html>
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| - | <!--
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| - | ============================================================================================
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| - | *** Acknowledgments ***
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| - | >>>To TU_Delf Team
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| - | Thank you so much to TU_Delft iGEM2010 team for providing us with so many examples of html code and how to apply them.
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| - | If you like some of this code please check it out in their wiki: https://2010.igem.org/Team:TU_Delft#page=Modeling/wiki-tips-tricks
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| - | ============================================================================================
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| - | <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
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| - | <title>Team: UANL_Mty-Mexico</title>
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| - | <META NAME="keywords" CONTENT="UANL,iGEM,Universidad,Autonoma,Nuevo,Leon,Biphasic,switch,Lambda,Genetic,Regulation,Quorum,Sensing,pRM,OL,Green Light,Red Light">
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| - | <META NAME="description" CONTENT="iGEM-UANL is the representative team from Universidad Autonoma de Nuevo León, at Monterrey, México. This team is composed of ten students who spent their summer in the lab, having fun with transformations, constructions and plasmidic DNA extractions. This">
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| - | <META NAME="abstract" CONTENT="Information processing through living things remains a challenge to science. Genetic logic-gates and">
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| - | <META NAME="author" CONTENT="iGEM-UANL">
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| - | <META NAME="robots" CONTENT="FOLLOW,INDEX">
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| - | <link href="http://www.genobiotec2011.org/iGEMwiki/mainStyle.css" rel="stylesheet" type="text/css">
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| - | <script type="text/javascript" src="http://www.genobiotec2011.org/iGEMwiki/Scripts/jquery-1.3.2.min.js"></script>
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| - | <script type="text/javascript" src="http://www.genobiotec2011.org/iGEMwiki/Scripts/jquery-1.3.2.min.js"></script>
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| - | <script type='text/javascript' src='http://www.genobiotec2011.org/iGEMwiki/jquery.easing.1.3.js'></script>
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| - | <script type='text/javascript' src='http://www.genobiotec2011.org/iGEMwiki/jquery.slideup.menu.1.0.min.js'></script>
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| - | <script type="text/javascript" src="http://www.genobiotec2011.org/iGEMwiki/Scripts/jquery.scroll-follow.js"></script>
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| - | <script type="text/javascript" src="http://www.genobiotec2011.org/iGEMwiki/Scripts/slimbox2/js/slimbox2.js"></script>
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| - | <script>
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| - | var opts = {slideUpSpeed:500, slideDownSpeed: 200};
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| - | $(document).ready(function(){
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| - | $('.top-menu').removeClass().addClass('top-menu').addClass('style5');
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| - | $('ul.s-menu li').click(function(){
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| - | $(this).parent().find('li').removeClass('selected');
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| - | $(this).addClass('selected');
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| - | });
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| - | $('ul.s-style li').click(function(){
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| - | $('.top-menu').removeClass().addClass('top-menu').addClass('style'+$(this).attr('rel'));
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| - | $('.top-menu').removeClass().addClass('top-menu').addClass('style5');
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| - | $(".top-menu").slideupmenu(opts);
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| - | $('ul.s-way li[rel="1"]').live('click', function() {
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| - | opts = {slideUpSpeed:500, slideDownSpeed: 200};
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| - | $(".top-menu").slideupmenu(opts);
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| - | $('ul.s-way li[rel="2"]').live('click', function() {
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| - | opts = {slideUpSpeed: 500, slideDownSpeed: 500, ease: "easeOutBounce", stopQueue: true};
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| - | $(".top-menu").slideupmenu(opts);
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| - | $('ul.s-way li[rel="3"]').live('click', function() {
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| - | opts = {slideUpSpeed: 1000, slideDownSpeed: 100, ease: "easeOutBack", stopQueue: true};
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| - | $(".top-menu").slideupmenu(opts);
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| - | opts = {slideUpSpeed: 500, slideDownSpeed: 500, ease:false, stopQueue: false};
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| - | $(".top-menu").slideupmenu(opts);
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| - | <script type="text/javascript">
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| - | $( document ).ready( function ()
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| - | $( '#rightColumn' ).scrollFollow();
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| - | </script>
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| - | </head>
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| - | <body>
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| - | <div class="content">
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| - | <div class="main" style="width:930px">
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| - | <!--<div style="width:900px">-->
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| - | <div class="br2"></div><div class="br2"></div><div class="br2"></div>
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| - | <div id="leftColumn">
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| - | <div id="ColorHeader">
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| - | Project: Overview
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| - | </div>
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| - | <div class="br"></div><div class="br"></div>
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| - | <div class="br"></div><div class="br"></div>
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| - | <div id="header-project-column">
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| - | <div class="br2"></div><div class="br2"></div><div class="br2"></div>
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| - | S.C.I.E.N.C.E. : Simple Code Interpretation Enabling Circuit in <i>E. coli</i>
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| - | <div class="br2"></div><div class="br2"></div><div class="br2"></div>
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| - | <p>Information processing through living things remains a challenge to science. Genetic logic-gates and switches have been used to this purpose<a href="#references" class="references-link">[1]</a>; however, most of these constructions use chemical inputs. Nonetheless, light induction systems have been constructed and characterized in the last few years<a href="#references" class="references-link">[2]</a>. Our project aims to enable a bacterial community, constituted by three <i>E. coli</i> strains that communicate through quorum sensing, to overall interpret a simple light based code. We attempt to insert the necessary genes for the light induction into <i>E. coli</i>'s chromosome, in order to create three different light responsive strains. Since light induction is becoming increasingly used in synthetic biology, we propose these modified <i>E. coli</i> strains as photochassis that could make useful tools in the field. </p><p> Furthermore, each strain will contain different plasmids carrying the genetic constructions needed for the interpretation of the code. This mechanism will mainly rely on genetic logic-gates and switches. The use of phage lambda's based biphasic switch<a href="#references" class="references-link">[3]</a>, which will theoretically allow the independent control of transcription from two different promoters through a single input, is introduced to iGEM.
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| - | </p>
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| - | <div class="br"></div>
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| - | <div id="header-project-column">
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| - | <div class="br2"></div><div class="br2"></div><div class="br2"></div>
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| - | iGEM-UANL Project video
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| - | <div class="br2"></div><div class="br2"></div><div class="br2"></div>
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| - | </div>
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| - | <p>This video deals with the following points:
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| - | <div class="br2"></div>
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| - | - Construction of two genetic <a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Contributions/Photochassis">photocassettes</a>, the induction systems for green and red lights.
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| - | <div class="br2"></div>
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| - | - Chromosome insertion of the previous constructions and birth of two new <i>E. coli</i> strains, which we dubbed <a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Contributions/Photochassis">photochassis</a>! (responsive to green and red lights, respectively).
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| - | <div class="br2"></div>
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| - | - DNA synthesis of the <a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Project/Mechanism">interpretation</a> genetic <a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Project/Circuit">circuits</a> by our dear sponsor <a href="http://www.genscript.com/" target="_new">GenScript</a> (not that we did not try traditional assembly). Note there are three different constructions, which will make three smart bacteria.
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| - | <div class="br2"></div>
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| - | - Finally the <a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Project/The_Code#Community">community</a> processing the information, sent through the <a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Project/The_Code">light-code</a> (inside the <a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Contributions/Light_Machine">light-machine</a>!). Note how the cells within the community work together through quorum sensing.
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| - | </p>
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| - | <iframe width="640" height="480" src="http://www.youtube.com/embed/E5GHK1-gaM4" frameborder="0" allowfullscreen></iframe>
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| - | </center>
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| - | <div class="br2"></div><div class="br2"></div><div class="br2"></div>
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| - | <a name="references"></a>References
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| - | <div class="br2"></div><div class="br2"></div><div class="br2"></div>
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| - | </div>
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| - | <ol><li> Tamsir A, Tabor JJ, Voigt CA (2010) Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. <i>Nature</i> <b>469</b>:212-215.
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| - | <li> Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in <i>Escherichia coli</i>. <i>J. Mol. Biol.</i> <b>405</b>:315-324.
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| - | </li><li> Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. <i>Gene Dev</i> <b>15</b>:3013–3022.
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| - | </li>
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| - | </li></ol>
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| - | <!-- <div id="social-div">
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| - | <a href="http://twitter.com/iGEMUANL" class="twitter-follow-button" data-show-count="false" data-align="right">Follow @iGEMUANL</a>
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| - | <script src="http://platform.twitter.com/widgets.js" type="text/javascript"></script>
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| - | <div class="br"></div>
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| - | <iframe src="http://www.facebook.com/plugins/like.php?app_id=134010103347967&href=http%3A%2F%2Fwww.facebook.com%2Fpages%2FiGEM-UANL%2F257596767587618&send=false&layout=button_count&width=200&show_faces=false&action=like&colorscheme=light&font&height=21" scrolling="no" frameborder="0" style="border:none; overflow:hidden; width:80px; height:21px;" allowTransparency="true">
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| - | <a href="#top"><img src="http://www.genobiotec2011.org/iGEMwiki/images/OurSymbol.png" width="200" height="160" alt="OurSymbol"></a>
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| - | <div class="lateral-button"><a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Project/The_Code">The Code</a></div>
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| - | <div class="lateral-button"><a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Project/Mechanism">Mechanism</a></div>
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| - | <div class="lateral-button"><a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Project/Circuit">Genetic Circuit</a></div>
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| - | <div class="lateral-button"><a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Project/Applications">Applications</a></div>
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| - | <p><img src="http://www.genobiotec2011.org/iGEMwiki/images/SymbolFooter.png" width="950" height="70" alt="OurSymbol"></p>
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| - | {{:Team:UANL_Mty-Mexico/Templates/Footer-template}}
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