Team:UANL Mty-Mexico/Project/Overview

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         <a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Project/Overview" class="menu-item-text">Project</a>
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           <li class=""><a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Achievements/Light_Machine">Light Machine</a></li>
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    <p>Information processing through living things remains a challenge to science. Genetic logic-gates and switches have been used to this purpose[1]; however, most of these constructions use chemical inputs. Nonetheless, light induction systems have been constructed and characterized in the last few years[2]. Our project aims to enable a bacterial community, constituted by three <i>E. coli</i> strains that communicate through quorum sensing, to overall interpret a simple light based code. We attempt to insert the necessary genes for the light induction into <i>E. coli</i>'s chromosome, in order to create three different light responsive strains.</p><p> Since light induction is becoming increasingly used in synthetic biology, we propose these modified <i>E. coli</i> strains as photochassis that could make useful tools in the field. Furthermore, each strain will contain different plasmids carrying the genetic constructions needed for the interpretation of the code. This mechanism will mainly rely on genetic logic-gates and switches. The use of phage lambda's based biphasic switch[3], which will theoretically allow the independent control of transcription from two different promoters through a single input, is introduced to iGEM.
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<ol><li> Tamsir A, Tabor JJ, Voigt CA. (2010). Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. <i>Nature</i>. <b>469</b>: 212-215.
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<li> Tabor JJ, Levskaya A, Voigt CA. (2010). Multichromatic Control of Gene Expression in <i>Escherichia coli</i>. <i>J. Mol. Biol.</i> <b>405</b>: 315-324.
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</li><li> Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ. (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny.  <i>Gene Dev</i>, <b>15</b>:3013–3022.
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  font-size: 10px;
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  line-height: 18px;
 +
}
 +
 
 +
.style5 ul.top-menu-main > li {
 +
  background: #000;
 +
  border-right: 1px solid #afafaf;
 +
 +
 
 +
  color: #fff;
 +
  margin: 0;
 +
}
 +
 
 +
.style5 ul.top-menu-main > li.last {
 +
  border-right: none;
 +
}
 +
.style5 ul.top-menu-main > li:hover .menu-item-text
 +
{color: #cfcfcf; text-shadow: 0 1px 0 #0C131C;}
 +
 
 +
.style5 ul.top-menu-main > li:hover{
 +
  background: #333 transparent;
 +
  color: #fff;
 +
  -moz-box-shadow: 0 0 8px #333;
 +
  -webkit-box-shadow: 0 0 8px #333;
 +
  box-shadow: 0 0 8px #333;
 +
}
 +
 
 +
.style5 ul.top-menu-main > li > a {
 +
  color: #fff;
 +
  text-decoration: none;
 +
}
 +
.style5 ul.top-menu-main > li > a:hover {
 +
  color: #fff;
 +
}
 +
 
 +
.style5 ul.top-menu-main > li > a {
 +
  padding: 10px 10px;
 +
}
 +
 
 +
.style5 ul.top-submenu {
 +
  -moz-box-shadow: 0 0 8px #333;
 +
  -webkit-box-shadow: 0 0 8px #333;
 +
  /*box-shadow: 0 0 8px #333;*/
 +
  box-shadow: 0 0 8px #fff;
 +
 
 +
}
 +
 
 +
.style5 ul.top-submenu > li {
 +
  margin: 0px;
 +
  padding: 5px 10px;
 +
  background: #fff transparent;
 +
 
 +
  color: #fff;
 +
  white-space: nowrap;
 +
 
 +
}
 +
 
 +
.style5 ul.top-submenu > li a {
 +
  margin: 0px;
 +
  padding: 5px 10px;
 +
  background: #fff transparent;
 +
 
 +
  color: #fff;
 +
  white-space: nowrap;
 +
 
 +
}
 +
 
 +
.style5 ul.top-submenu > li.hover {
 +
  background: #000 ;
 +
  color: #fff;
 +
 
 +
}
 +
 
 +
.style5 ul.top-submenu > li a:hover {
 +
  background: #000 ;
 +
  color: #fff;
 +
 
 +
}
 +
 
 +
 
 +
/* Wiki Hacks - START */
 +
/* Author: Pieter van Boheemen */
 +
/* Team: TU Delft */
 +
 
 +
#globalWrapper { background-color: transparent; border: none; margin: 0; padding: 0; width: 100%; height:100%;}
 +
 
 +
#content { background-color: transparent; border: none; padding: 0; margin: 0; width: 100%; overflow: hidden; height:100%;}
 +
 
 +
#bodyContent { border: none; padding:0; margin:0; width:100%; height:100%;}
 +
 
 +
#top-section { height: 0px; margin: 0px; margin-left: auto; margin-right: auto; margin-bottom: 0 !important;
 +
padding:0; border: none; font-size: 10px}
 +
 
 +
#p-logo { height:1px; overflow:hidden; display: none;}
 +
 
 +
#search-controls { overflow:hidden; display:block; background: none; position: absolute; top: 100px; right: 40px;}
 +
 
 +
/*
 +
.left-menu { width: 500px !important; display:block; margin-top:-80px; border: none; text-align: right;}
 +
.left-menu ul { border: none; }
 +
#menubar.right-menu { width:300px; display:block; float:left; margin-top:-80px; border: none;}
 +
.right-menu ul { border: none; width: 300px;}
 +
*/
 +
 
 +
#footer-box { background-image: url(http://www.genobiotec2011.org/iGEMwiki/images/back.jpg); background-color: #000000; width: 100%; margin: -10px; padding: 20px 0; display: none;}
 +
 
 +
.visualClear { display: none; }
 +
 
 +
#search-controls { display:none; }
 +
 
 +
h3#siteSub { display: none;}
 +
#contentSub {display: none;}
 +
//p:first-child { display: none;}
 +
 
 +
 
 +
#footer { border: none; width: 965px; margin: 0 auto; padding: 0;}
 +
.firstHeading { display: none;}
 +
#f-list a { color: #333; font-size: 10px;}
 +
#f-list a:hover { color: #666; height: 100px;}
 +
.printfooter { display: none; }
 +
#footer ul { margin: 0; padding: 0;}
 +
#footer ul li { margin-top: 0; margin-bottom: 0; margin-left: 10px; margin-right: 10px; padding: 0;}
 +
/* Wiki Hacks - END */
 +
 
 +
 
 +
body {
 +
margin: 0;
 +
padding: 0;
 +
color: #333;
 +
font-family: tahoma, arial;
 +
font-size: 10px;
 +
background-color: #333;
 +
background-image: url(images/back.jpg);
 +
}
 +
 
 +
a {
 +
color: #fff;
 +
text-decoration: none;
 +
font-size: 10px;
 +
}
 +
 
 +
/* float clearing for IE6 */
 +
* html .clearfix{
 +
  height: 1%;
 +
  overflow: visible;
 +
}
 +
 
 +
/* float clearing for IE7 */
 +
*+html .clearfix{
 +
  min-height: 1%;
 +
}
 +
 
 +
/* float clearing for everyone else */
 +
.clearfix:after{
 +
clear: both;
 +
content: ".";
 +
display: block;
 +
height: 0;
 +
visibility: hidden;
 +
font-size: 0;
 +
color: #066;
 +
}
 +
 
 +
ul.top-menu-main {
 +
  margin: auto;
 +
  width: 800px;
 +
}
 +
 
 +
ul.top-menu-main > li {
 +
  margin: 0 10px;
 +
}
 +
 
 +
.top-menu {
 +
  font-family: "Arial Narrow", Georgia, Tahoma, arial;
 +
  text-transform: uppercase;
 +
  font-size: 14px;
 +
  font-weight: bold;
 +
  background: #fff;
 +
  height: 37px;
 +
  border-bottom: 1px solid #333;
 +
}
 +
 
 +
.header {
 +
width: 964px;
 +
height: 203px;
 +
margin: auto;
 +
-moz-box-shadow: 0 0 8px #777;
 +
-webkit-box-shadow: 0 0 8px #777;
 +
box-shadow: 0 -5px 10px #777;
 +
z-index: 0;
 +
}
 +
 
 +
.content {
 +
width: 964px;
 +
margin: auto;
 +
background-color: #FFF;
 +
font-size: 12px;
 +
z-index: 10;
 +
text-align: center;
 +
padding-bottom: 10px;
 +
}
 +
 
 +
 
 +
.content3 {
 +
width: 950px;
 +
margin: auto;
 +
font-size: 10px;
 +
border-bottom-color: #0C0;
 +
color: #FFF;
 +
text-align: center;
 +
padding-top: 5px;
 +
padding-right: 0px;
 +
padding-bottom: 0;
 +
padding-left: 0px;
 +
}
 +
 
 +
.content .main {
 +
padding-top: 0px;
 +
padding-right: 10px;
 +
padding-bottom: 10px;
 +
padding-left: 10px;
 +
}
 +
 
 +
.donate {
 +
  padding: 5px;
 +
  background: #cfcfcf;
 +
  color: #333;
 +
  -webkit-border-radius: 5px;
 +
  -moz-border-radius: 5px;
 +
  border-radius: 5px;
 +
 
 +
}
 +
.donate > div {
 +
  padding: 10px;
 +
  position: relative; float: left; width : 700px;
 +
  font-style: italic;
 +
}
 +
 
 +
.s-menu {
 +
margin: 0;
 +
padding: 0;
 +
margin-top: 20px;
 +
color: #036;
 +
  text-decoration: none;
 +
}
 +
 
 +
.s-menu li {
 +
  text-decoration: none;
 +
list-style-type: none;
 +
width: 100px;
 +
padding-top: 10px;
 +
padding-bottom: 10px;
 +
padding-left: 10px;
 +
padding-right: 10px;
 +
margin: 2px;
 +
  -webkit-border-radius: 5px;
 +
  -moz-border-radius: 5px;
 +
  border-radius: 5px;
 +
  cursor: pointer;
 +
  text-align: center;
 +
width: 180px;
 +
}
 +
 
 +
.s-menu li.last {
 +
background: #cfcfcf;
 +
color: #000;
 +
text-align: center;
 +
list-style-type: none;
 +
}
 +
 
 +
.s-menu li:hover {
 +
background: #cfcfcf;
 +
color: #000;
 +
text-align: center;
 +
list-style-type: none;
 +
}
 +
 
 +
.s-menu li a {
 +
list-style-type: none;
 +
width: 100px;
 +
padding-top: 10px;
 +
padding-bottom: 10px;
 +
padding-left: 10px;
 +
padding-right: 10px;
 +
margin: 2px;
 +
-webkit-border-radius: 5px;
 +
-moz-border-radius: 5px;
 +
border-radius: 5px;
 +
cursor: pointer;
 +
text-align: center;
 +
color: #036;
 +
font-size: 12px;
 +
 
 +
 
 +
}
 +
.s-menu li:hover a {
 +
background: #cfcfcf;
 +
color: #000;
 +
text-align: center;
 +
font-size: 12px;
 +
}
 +
 
 +
 
 +
 
 +
.col-1-3 {
 +
width: 200;
 +
/*margin-right: 2%;*/
 +
position: relative;
 +
float: right;
 +
font-size: 12px;
 +
color: #FFF;
 +
font-weight: bold;
 +
text-decoration: none;
 +
 
 +
 
 +
 +
}
 +
 
 +
 
 +
 
 +
 
 +
.sidebar1 {
 +
float: left;
 +
width: 180px;
 +
background: #EADCAE;
 +
padding-bottom: 10px;
 +
}
 +
 
 +
.sidebar2 {
 +
float: right;
 +
width: 180px;
 +
background: #EADCAE;
 +
padding-bottom: 10px;
 +
}
 +
.content .main .clearfix .content2 {
 +
text-align: justify;
 +
width: 750px;
 +
font-size: 14px;
 +
border-left-color: #000;
 +
}
 +
.content .main .clearfix .content2 title {
 +
font-size: 20px;
 +
}
 +
 
 +
 
 +
#footer-leftColumn {
 +
float:left;
 +
width:317px;
 +
padding-left: 0px;
 +
text-align: left;
 +
padding-top: 0;
 +
padding-right: 0;
 +
padding-bottom: 0;
 +
}
 +
 
 +
#footer-middleColumn {
 +
float:left;
 +
width:330px;
 +
padding-left: 0px;
 +
text-align: center;
 +
padding-top: 0;
 +
padding-right: 0;
 +
padding-bottom: 0;
 +
}
 +
 
 +
#footer-rightColumn {
 +
float:right;
 +
width:260px;
 +
padding-left: 0px;
 +
text-align: right;
 +
padding-top: 0;
 +
padding-right: 0;
 +
padding-bottom: 0;
 +
}
 +
 
 +
#twitter-box {
 +
text-align:center;
 +
width: 200px;
 +
 
 +
}
 +
 
 +
#clustrmaps-widget {
 +
text-align:center;
 +
width:200px;
 +
padding-left: 50px;
 +
}
 +
 
 +
#leftColumn {
 +
float:left;
 +
width:700px;
 +
text-align: justify;
 +
padding-top: 10px;
 +
padding-right: 0px;
 +
padding-bottom: 0px;
 +
padding-left: 0px;
 +
font-size: 15px;
 +
font-family: Arial;
 +
}
 +
 
 +
#rightColumn {
 +
float:right;
 +
width:200px;
 +
text-align: center;
 +
padding-top: 5px;
 +
}
 +
 
 +
#social-div {
 +
text-align: right;
 +
float:right;
 +
}
 +
 
 +
#twitter-top {
 +
text-align: right;
 +
 +
}
 +
 
 +
#footer-middleColumn a {
 +
color: #ffffff;
 +
text-decoration:underline;
 +
font-weight:bolder;
 +
}
 +
 
 +
#youtube-frame {
 +
text-align:center;
 +
 +
}
 +
 
 +
#leftColumn1 {
 +
 +
}
 +
 
 +
#ColorHeader {
 +
background-color:#FFF;
 +
color: #333;
 +
padding-top: 5;
 +
padding-right: 5;
 +
padding-bottom: 5;
 +
padding-left: 5;
 +
font-family: "Arial Black";
 +
font-size: 30px;
 +
}
 +
 
 +
#project-column p {
 +
background-color:#FFF;
 +
color:#000;
 +
font: Tahoma;
 +
font-size: 14px;
 +
}
 +
 
 +
#project-column {
 +
width:340px;
 +
padding-top: 15;
 +
padding-right: 15;
 +
padding-bottom: 5;
 +
padding-left: 0;
 +
float: left;
 +
}
 +
 
 +
#header-project-column {
 +
background-color:#036;
 +
color: #FFF;
 +
padding-top: 5;
 +
padding-right: 5;
 +
padding-bottom: 5;
 +
padding-left: 10px;
 +
font-family: "Arial Black";
 +
font-size: 16px;
 +
text-align:left;
 +
}
 +
 
 +
#content-project-column {
 +
 +
}
 +
 
 +
#About-column {
 +
float:left;
 +
width:320px;
 +
padding-top: 15;
 +
padding-right: 5;
 +
padding-bottom: 5;
 +
padding-left: 15px;
 +
}
 +
#header-about-column {
 +
background-color:#036;
 +
color: #FFF;
 +
padding-top: 5;
 +
padding-right: 5;
 +
padding-bottom: 5;
 +
padding-left: 10px;
 +
font-family: "Arial Black";
 +
font-size: 16px;
 +
text-align:left;
 +
}
 +
 
 +
#content-about-column {
 +
 +
}
 +
 
 +
#content-about-column p {
 +
background-color:#FFF;
 +
color:#000;
 +
font-size: 15px;
 +
font-family: Arial;
 +
}
 +
 
 +
#content-about-column a {
 +
color:#036;
 +
font-size: 14px;
 +
text-decoration:underline;
 +
font-family: Tahoma;
 +
font-weight: bolder;
 +
}
 +
 
 +
 
 +
#content-3 {
 +
 +
}
 +
 
 +
#header-content-3 {
 +
background-color:#333;
 +
color: #FFF;
 +
padding-top: 5;
 +
padding-right: 5;
 +
padding-bottom: 5;
 +
padding-left: 10px;
 +
font-family: "Arial Black";
 +
font-size: 16px;
 +
text-align:left;
 +
}
 +
 
 +
#content-project-column a {
 +
color:#036;
 +
font-size: 14px;
 +
text-decoration:underline;
 +
font-family: Tahoma;
 +
font-weight: bolder;
 +
font-style: italic;
 +
}
 +
 
 +
#outline-header {
 +
background-color:#036;
 +
color: #FFF;
 +
padding-top: 5;
 +
padding-right: 5;
 +
padding-bottom: 5;
 +
padding-left: 10px;
 +
font-family: "Arial Black";
 +
font-size: 16px;
 +
}
 +
 
 +
#outline-content {
 +
 +
}
 +
 
 +
 
 +
 
 +
 
 +
<!-- TU_Delft stqrts here -->
 +
 
 +
 
 +
 
 +
#TUD-footer-content div {
 +
line-height: 12px;
 +
}
 +
 
 +
 
 +
 
 +
.br {
 +
height: 5px;
 +
width: 100%;
 +
margin: 0;
 +
padding: 0;
 +
overflow: hidden;
 +
}
 +
 
 +
.br2 {
 +
height: 2px;
 +
width: 100%;
 +
margin: 0;
 +
padding: 0;
 +
overflow: hidden;
 +
}
 +
 
 +
.content .main #leftColumn p a {
 +
color: #00C;
 +
font-size: 15px;
 +
}
 +
 
 +
.content .main #leftColumn p a:hover {
 +
color: #00C;
 +
font-size: 15px;
 +
text-decoration:underline;
 +
}
 +
 
 +
 
 +
 
 +
.TUD-footer-title {
 +
font-weight: bold;
 +
color: #019aed;
 +
}
 +
 
 +
#TUD-footer-search form {
 +
margin: 0;
 +
padding: 0;
 +
font-size: 10px;
 +
}
 +
 
 +
#TUD-footer-search form #searchInput {
 +
background-color: #4db7f1;
 +
border: 1px solid #000;
 +
width: 100%;
 +
}
 +
 
 +
#TUD-footer-search form .searchButton {
 +
margin-top: 4px;
 +
font-size: 11px;
 +
background-color: #266c96;
 +
border: 1px solid #000;
 +
color: #4db7f1;
 +
padding: 2px;
 +
}
 +
 
 +
 
 +
 
 +
<!-- TU_Delft ends here -->
 +
 
 +
 
 +
 
 +
</style>
 +
 
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
 +
  <title>Team: UANL_Mty-Mexico</title>
 +
 
 +
  <script type='text/javascript' src='http://www.genobiotec2011.org/iGEMwiki/jquery.easing.1.3.js'></script>
 +
  <script type='text/javascript' src='http://www.genobiotec2011.org/iGEMwiki/jquery.slideup.menu.1.0.min.js'></script>
 +
 
 +
<script>
 +
    var opts = {slideUpSpeed:500, slideDownSpeed: 200};
 +
    $(document).ready(function(){
 +
$('.top-menu').removeClass().addClass('top-menu').addClass('style5');
 +
      $('ul.s-menu li').click(function(){
 +
        $(this).parent().find('li').removeClass('selected');
 +
        $(this).addClass('selected');
 +
      });
 +
      $('ul.s-style li').click(function(){
 +
      $('.top-menu').removeClass().addClass('top-menu').addClass('style'+$(this).attr('rel'));
 +
      $('.top-menu').removeClass().addClass('top-menu').addClass('style5');
 +
 
 +
        $(".top-menu").slideupmenu(opts); 
 +
      });
 +
     
 +
      $('ul.s-way li[rel="1"]').live('click', function() {
 +
        opts = {slideUpSpeed:500, slideDownSpeed: 200};
 +
        $(".top-menu").slideupmenu(opts); 
 +
      });
 +
      $('ul.s-way li[rel="2"]').live('click', function() {
 +
        opts = {slideUpSpeed: 500, slideDownSpeed: 500, ease: "easeOutBounce", stopQueue: true};
 +
        $(".top-menu").slideupmenu(opts); 
 +
      });
 +
      $('ul.s-way li[rel="3"]').live('click', function() {
 +
        opts = {slideUpSpeed: 1000, slideDownSpeed: 100, ease: "easeOutBack", stopQueue: true};
 +
        $(".top-menu").slideupmenu(opts); 
 +
      });
 +
      $('ul.s-way li[rel="4"]').live('click', function() {
 +
        opts = {slideUpSpeed: 500, slideDownSpeed: 500, ease:false, stopQueue: false};
 +
        $(".top-menu").slideupmenu(opts); 
 +
      });
 +
      $(".top-menu").slideupmenu(opts); 
 +
    });
 +
  </script>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<style type="text/css">
 +
body {
 +
background-color: #000;
 +
position: 950;
 +
}
 +
.header1 {
 +
width: 950px;
 +
height: 203px;
 +
margin: auto;
 +
-moz-box-shadow: 0 0 8px #777;
 +
-webkit-box-shadow: 0 0 8px #777;
 +
box-shadow: 0 -5px 10px #777;
 +
}
 +
.textblack {
 +
color: #000;
 +
}
 +
footer-middleColumn-text {
 +
text-decoration: underline;
 +
font-size: 10px;
 +
font-weight: bolder;
 +
}
 +
 
 +
</style>
 +
 
 +
 
 +
</head>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
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Revision as of 23:49, 16 September 2011

Team: UANL_Mty-Mexico

banner-main iGEM-logo
Project
Overview

Information processing through living things remains a challenge to science. Genetic logic-gates and switches have been used to this purpose[1]; however, most of these constructions use chemical inputs. Nonetheless, light induction systems have been constructed and characterized in the last few years[2]. Our project aims to enable a bacterial community, constituted by three E. coli strains that communicate through quorum sensing, to overall interpret a simple light based code. We attempt to insert the necessary genes for the light induction into E. coli's chromosome, in order to create three different light responsive strains.

Since light induction is becoming increasingly used in synthetic biology, we propose these modified E. coli strains as photochassis that could make useful tools in the field. Furthermore, each strain will contain different plasmids carrying the genetic constructions needed for the interpretation of the code. This mechanism will mainly rely on genetic logic-gates and switches. The use of phage lambda's based biphasic switch[3], which will theoretically allow the independent control of transcription from two different promoters through a single input, is introduced to iGEM.

References
  1. Tamsir A, Tabor JJ, Voigt CA. (2010). Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature. 469: 212-215.
  2. Tabor JJ, Levskaya A, Voigt CA. (2010). Multichromatic Control of Gene Expression in Escherichia coli. J. Mol. Biol. 405: 315-324.
  3. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ. (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Gene Dev, 15:3013–3022.

OurSymbol

Team: UANL_Mty-Mexico
banner-main iGEM-logo
Project
Overview

Information processing through living things remains a challenge to science. Genetic logic-gates and switches have been used to this purpose[1]; however, most of these constructions use chemical inputs. Nonetheless, light induction systems have been constructed and characterized in the last few years[2]. Our project aims to enable a bacterial community, constituted by three E. coli strains that communicate through quorum sensing, to overall interpret a simple light based code. We attempt to insert the necessary genes for the light induction into E. coli's chromosome, in order to create three different light responsive strains.

Since light induction is becoming increasingly used in synthetic biology, we propose these modified E. coli strains as photochassis that could make useful tools in the field. Furthermore, each strain will contain different plasmids carrying the genetic constructions needed for the interpretation of the code. This mechanism will mainly rely on genetic logic-gates and switches. The use of phage lambda's based biphasic switch[3], which will theoretically allow the independent control of transcription from two different promoters through a single input, is introduced to iGEM.

References
  1. Tamsir A, Tabor JJ, Voigt CA. (2010). Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature. 469: 212-215.
  2. Tabor JJ, Levskaya A, Voigt CA. (2010). Multichromatic Control of Gene Expression in Escherichia coli. J. Mol. Biol. 405: 315-324.
  3. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ. (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Gene Dev, 15:3013–3022.

OurSymbol

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