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Team:UANL Mty-Mexico/Project/Overview
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<i>Av. Manuel L. Barragan S/N, Cd. Universitaria. C.P.66450. San Nicolás de los Garza, Nuevo León, México.</i> | <i>Av. Manuel L. Barragan S/N, Cd. Universitaria. C.P.66450. San Nicolás de los Garza, Nuevo León, México.</i> | ||
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Revision as of 06:34, 13 September 2011
Information processing through living things remains a challenge to science. Genetic logic-gates and switches have been used to this purpose[1]; however, most of this constructions use chemical inputs. Nonetheless, light induction systems have been constructed and characterized in the last few years[2]. Our project aims to enable a bacterial community, constituted by three E. coli strains that communicate through quorum sensing, to overall interpret a simple light based code. We attempt to insert the necessary genes for the light induction into E. coli 's chromosome, in order to create three different light responsive strains.
Since light induction is becoming increasingly used in synthetic biology, we propose these modified E. coli strains as photochassis that could make useful tools in the field. Furthermore, each strain will contain different plasmids carrying the genetic constructions needed for the interpretation of the code. This mechanism will mainly rely on genetic logic-gates and switches. The use of phage lambda's based biphasic switch[4], which will theoretically allow the independent control of transcription from two different promoters through a single input, is introduced to iGEM.
- Tamsir A, Tabor JJ, Voigt CA. (2010). Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature. 469: 212-215.
- Tabor JJ, Levskaya A, Voigt CA. (2010). Multichromatic Control of Gene Expression in Escherichia coli. J. Mol. Biol. 405: 315-324.
- Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ. (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny Gene Dev, 15:3013–3022.
