Team:TzuChiU Formosa/Notebook/photopaper

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</html>
</html>
<font color="#228B22" size=6>Photopaper</font>
<font color="#228B22" size=6>Photopaper</font>
-
<br><br><font color="#000080" size=4>Meeting Notes</font>
+
<br><br><font color="#000080" size=4>Meeting Minutes</font>
<Hr Align="left" width="100%" size=2>
<Hr Align="left" width="100%" size=2>
<font color="#000000" size=3><b>2011.02.24</b></font>
<font color="#000000" size=3><b>2011.02.24</b></font>
Line 145: Line 145:
<Hr Align="left" width="100%" size=3>
<Hr Align="left" width="100%" size=3>
-
<font size=3><b>2011.09.07</b></font>
 
-
<br>[[File:Catt.gif|right|250px|caption]]
 
-
<b>Rhodobacter rudrum medium</b>
 
-
<br>K2HPO4             1g<br>
 
-
<br>NaCl              0.5g<br>
 
-
<br>FeSO4.7H2O          0.01g<br>
 
-
<br>CaCl2              0.02g<br>
 
-
<br>MnCl2.4H2O          0.002g<br>
 
-
<br>MgSO4.7H2O          0.2g<br>
 
-
<br>NaMO2O4.2H2O         0.01g<br>
 
-
<br>ddH2O              998.258ml<br>
 
-
<br>________________________________________
 
-
<br>                 1L →take100ml<br>
 
-
 
-
          + 
 
-
<br>Yeast Extrat                0.5g<br>
+
<br>[[File:1-20110907.jpg|lefe|650px|caption]][[File:Catt.gif|right|250px|caption]]
-
<br>Sodium malate
+
-
<br>(Sodium succinate dibasic hexohydrate)    5g<br>
+
-
<br>NH4Cl                          1g<br>
+
-
<br>ddH2O                         893.5ml<br>
+
-
<br>_________________________________________________
+
-
<br>                            1L<br>
+
<br>
<br>
<br>
<br>
-
<br><font color="#000000" size=2><b>Raise E. coli(PSB1C3)</B></font>
 
<br>
<br>
-
:: 1.50ml LB+500μl CHLORAMPHENICOL
+
<br>[[File:2-20110908-13.jpg|lefe|650px|caption]]
-
:: 2.37℃, overnight (14-16hrs)
+
<br>
<br>
<br>
<br>
<br>
<br>
-
<br><font color="#000000" size=3><b>2011.09.08-13</b></font>
+
<br>[[File:3-20110910-11.jpg|lefe|650px|caption]]
<br>
<br>
-
<br><font color="#000000" size=2><b>Plasmid miniprep kit</b></font>
 
-
*PSB1C3 plasmid[[File:Cats.jpg|right|404px|caption]]
 
<br>
<br>
<br>
<br>
-
<br><font color="#000000" size=2><b>Raise Rhodobacter rubrum</b></font>
+
<br>[[File:4-20110912-20.jpg|lefe|650px|caption]]
-
:: 1.50ml LB+500μl CHLORAMPHENICOL
+
-
:: 2.37℃, overnight (14-16hrs)
+
<br>
<br>
<br>
<br>
<br>
<br>
-
<br><font color="#000000" size=3><b>2011.09.09</b></font>
+
<br>[[File:5-20110921.jpg|lefe|550px|caption]][[File:Cats.jpg|right|404px|caption]]
<br>
<br>
-
<br><font color="#000000" size=2><b>Raise Gluconacetobacter hansenii</b></font>
 
-
:: 1.50ml LB+500μl CHLORAMPHENICOL
 
-
:: 2.37℃, overnight (14-16hrs)
 
<br>
<br>
<br>
<br>
 +
<br>[[File:6-20110922.jpg|lefe|650px|caption]]
<br>
<br>
-
<br><font color="#000000" size=3><b>2011.09.10-11</b></font>
 
<br>
<br>
-
<br><font color="#000000" size=2><b>Digestion check of DNA</b></font>
 
<br>
<br>
 +
<br>[[File:7-20110923.jpg|lefe|650px|caption]]
<br>
<br>
-
[pSB1C3/EcoRI]
 
-
<br>DNA            500ng<br>
 
-
<br>10×buffer             5μl<br>
 
-
<br>BSA             5μl<br>
 
-
<br>EcoRⅠ            1μl<br>
 
-
<br>ddH2O           29μl<br>
 
-
<br>_______________________________
 
<br>
<br>
-
<br>total           50μl<br>
 
<br>
<br>
 +
<br>[[File:8-20110924.jpg|lefe|550px|caption]][[File:Cats2.gif |right|404px|caption]]
<br>
<br>
<br>
<br>
-
[pSB1C3/PstⅠ]
 
-
<br>DNA             500ng<br>
 
-
<br>10×buffer           5μl<br>
 
-
<br>BSA               5μl<br>
 
-
<br>PstⅠ              1μl<br>
 
-
<br>ddH2O             29μl<br>                                             
 
-
<br>_________________________________
 
<br>
<br>
-
<br>total             50μl<br>
+
<br>[[File:9-20110925.jpg|lefe|650px|caption]]
<br>
<br>
<br>
<br>
-
<br><font color="#000000" size=2><b>Digestion of DNA</b></font>
 
<br>
<br>
 +
<br>[[File:10-20110926.jpg|lefe|650px|caption]]
<br>
<br>
-
[pSB1C3/EcoRⅠ+PstⅠ]
 
-
<br>DNA             500ng<br>
 
-
<br>10×buffer           5μl<br>
 
-
<br>BSA               5μl<br>
 
-
<br>EcoRⅠ              1μl<br>
 
-
<br>pstⅠ              1μl<br>
 
-
<br>ddH2O            28μl<br>             
 
-
<br>__________________________________
 
<br>
<br>
-
<br>total            50μl<br>
 
-
<br>→37℃ for 30 mins
 
<br>
<br>
 +
<br>[[File:11-20110927.jpg|lefe|550px|caption]][[File:Cats3.jpg|right|404px|caption]]
<br>
<br>
<br>
<br>
-
[pSB1C3/XbaⅠ+SpeⅠ]
 
-
<br>DNA               10μl<br>
 
-
<br>10×buffer            5μl<br>
 
-
<br>BSA               5μl<br>
 
-
<br>XbaⅠ                1μl<br>
 
-
<br>SpeⅠ               1μl<br>
 
-
<br>ddH2O              28μl<br>
 
-
<br>____________________________________
 
<br>
<br>
-
<br>total              50μl<br>
+
<br>[[File:12-20110928.jpg|lefe|650px|caption]]
-
<br>→37℃ for 2 hrs
+
<br>
<br>
<br>
<br>
<br>
<br>
-
[pSB1C3/SpeⅠ+PstⅠ]
+
<br>[[File:13-20110929.jpg|lefe|650px|caption]]
-
<br>DNA               10μl<br>
+
-
<br>10×buffer            5μl<br>
+
-
<br>BSA               5μl<br>
+
-
<br>SpeⅠ              1μl<br>
+
-
<br>pstⅠ               1μl<br>
+
-
<br>ddH2O              28μl<br>
+
-
<br>____________________________________
+
<br>
<br>
-
<br>total              50μl<br>
 
-
<br>→37℃ for 2 hrs
 
<br>
<br>
<br>
<br>
-
<br>
+
<br>[[File:14-20111001.jpg|lefe|650px|caption]]
-
[pSB1C3/EcoRⅠ+XbaⅠ]
+
-
<br>DNA               10μl<br>
+
-
<br>10×buffer            5μl<br>
+
-
<br>BSA               5μl<br>
+
-
<br>EcoRⅠ              1μl<br>
+
-
<br>XbaⅠ               1μl<br>
+
-
<br>ddH2O              28μl<br>
+
-
<br>____________________________________
+
-
<br>
+
-
<br>total              50μl<br>
+
-
<br>→37℃ for 2 hrs
+
-
<br>
+
-
<br>
+
-
<br>
+
-
[pSB1A3/EcoRⅠ+PstⅠ]
+
-
<br>DNA               10μl<br>
+
-
<br>10×buffer            5μl<br>
+
-
<br>BSA               5μl<br>
+
-
<br>EcoRⅠ              1μl<br>
+
-
<br>PstⅠ               1μl<br>
+
-
<br>ddH2O              28μl<br>
+
-
<br>____________________________________
+
-
<br>
+
-
<br>total              50μl<br>
+
-
<br>→37℃ for 2 hrs
+
-
<br>
+
-
<br>
+
-
[pSB1A3/EcoRⅠ+SpeⅠ]
+
-
<br>DNA               10μl<br>
+
-
<br>10×buffer            5μl<br>
+
-
<br>BSA               5μl<br>
+
-
<br>EcoRⅠ              1μl<br>
+
-
<br>SpeⅠ               1μl<br>
+
-
<br>ddH2O              28μl<br>
+
-
<br>____________________________________
+
-
<br>
+
-
<br>total              50μl<br>
+
-
<br>→37℃ for 2 hrs
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>electroelution Purification</b></font>
+
-
<br>
+
-
<br>
+
-
*[pSB1C3/EcoRⅠ+PstⅠ]
+
-
*[pSB1C3/XbaⅠ+SpeⅠ]
+
-
*[pSB1C3/SpeⅠ+PstⅠ]
+
-
*[pSB1C3/EcoRⅠ+XbaⅠ]
+
-
*[pSB1A3/EcoRⅠ+PstⅠ]
+
-
*[pSB1A3/EcoRⅠ+SpeⅠ]
+
-
<br>
+
-
<br>
+
-
 
+
-
<br><font color="#000000" size=3><b>2011.09.12-20</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>PCR</b></font>
+
-
[[File:Cats2.gif |right|404px|caption]]
+
-
<br>
+
-
*[acsAB]
+
-
*[acsCD]
+
-
*[CCcax-Ccp]
+
-
<br>template DNA   1μl<br>
+
-
<br>5×Buffer     4μl<br>
+
-
<br>2.5μM dNTP    1.6μl<br>
+
-
<br>10μM F      1μl<br>
+
-
<br>10μM R      1μl<br>
+
-
<br>Taq        0.2μl<br>
+
-
<br>ddH2O      8.8μl<br>
+
-
<br>_______________________________
+
-
<br>
+
-
<br>total       20μl<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>electroelution Purification</b></font>
+
-
<br>
+
-
<br>
+
-
*[acsAB]
+
-
*[acsCD]
+
-
*[CMCax-Ccp]
+
-
<br>
+
-
<br>
+
-
<br>
+
-
 
+
-
<br><font color="#000000" size=3><b>2011.09.21</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Digestion of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
[acsAB/ XbaⅠ+SpeⅠ]
+
-
<br>DNA           10μl<br>
+
-
<br>10×buffer        5μl<br>
+
-
<br>BSA           5μl<br>
+
-
<br>EcoRⅠ          1μl<br>
+
-
<br>pstⅠ           1μl<br>
+
-
<br>ddH2O          28μl<br>
+
-
<br>____________________________
+
-
<br>
+
-
<br>total             50μl<br>
+
-
<br>→37℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
[acsCD/XbaⅠ+SpeⅠ]
+
-
<br>DNA            10μl<br>
+
-
<br>10×buffer         5μl<br>
+
-
<br>BSA             5μl<br>
+
-
<br>XbaⅠ            1μl<br>
+
-
<br>SpeⅠ             1μl<br>
+
-
<br>ddH2O            28μl<br>
+
-
<br>__________________________________
+
-
<br>
+
-
total               50μl<br>
+
-
<br>→37℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
[CMCax/SpeⅠ+AlwNⅠ]
+
-
<br>DNA            10μl<br>
+
-
<br>10×buffer         5μl<br>
+
-
<br>BSA             5μl<br>
+
-
<br>SpeⅠ            1μl<br>
+
-
<br>AlwNⅠ             1μl<br>
+
-
<br>ddH2O            28μl<br>
+
-
<br>__________________________________
+
-
<br>
+
-
<br>total               50μl<br>
+
-
<br>→37℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
[Ccp/AlwNⅠ+PstⅠ]
+
-
<br>DNA            10μl<br>
+
-
<br>10×buffer         5μl<br>
+
-
<br>BSA             5μl<br>
+
-
<br>AlwNⅠ            1μl<br>
+
-
<br>PstⅠ             1μl<br>
+
-
<br>ddH2O            28μl<br>
+
-
<br>__________________________________
+
-
<br>
+
-
total               50μl<br>
+
-
<br>→37℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>electroelution Purification</b></font>
+
-
<br>
+
-
<br>
+
-
*[acsAB/ XbaⅠ+SpeⅠ]
+
-
*[acsCD/XbaⅠ+SpeⅠ]
+
-
*[CMCax/SpeⅠ+AlwNⅠ]
+
-
*[Ccp/AlwNⅠ+PstⅠ]
+
-
<br>
+
-
<br>
+
-
 
+
-
<br><font color="#000000" size=3><b>2011.09.22</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Ligation of DNA</b></font>
+
-
<br>
+
-
[pSB1C3-acsAB][[File:Cats3.jpg|right|404px|caption]]
+
-
<br>Vector          3μl<br>
+
-
<br>Insert          14μl<br>
+
-
<br>ligase buffer        2μl<br>
+
-
<br>ligase          1μl<br>
+
-
<br>ddH2O           -μl<br>
+
-
<br>________________________________
+
-
<br>
+
-
<br>total          20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
[pSB1A3-acsCD]
+
-
<br>Vector           3μl<br>
+
-
<br>Insert           14μl<br>
+
-
<br>ligase buffer       2μl<br>
+
-
<br>ligase            1μl<br>
+
-
<br>ddH2O             -μl<br>
+
-
<br>_________________________________
+
-
<br>
+
-
<br>total           20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
[pSB1C3-acsCD]
+
-
<br>Vector           3μl<br>
+
-
<br>Insert           14μl<br>
+
-
<br>ligase buffer       2μl<br>
+
-
<br>ligase            1μl<br>
+
-
<br>ddH2O             -μl<br>
+
-
<br>_________________________________
+
-
<br>
+
-
<br>total           20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
[pSB1C3-CMCax-Ccp]
+
-
<br>Vector           3μl<br>
+
-
<br>Insert           14μl<br>
+
-
<br>ligase buffer       2μl<br>
+
-
<br>ligase            1μl<br>
+
-
<br>ddH2O             -μl<br>
+
-
<br>_________________________________
+
-
<br>
+
-
<br>total           20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
 
+
-
<br><font color="#000000" size=3><b>2011.09.23</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>PCR</b></font>
+
-
<br>
+
-
<br>
+
-
[R0011 promoter]
+
-
<br>R0011 promoter         1μl<br>
+
-
<br>5×Buffer            4μl<br>
+
-
<br>2.5μM dNTP          1.6μl<br>
+
-
<br>Taq               0.2μl<br>
+
-
<br>ddH2O             13.2μl<br>
+
-
<br>_____________________________________
+
-
<br>
+
-
<br>total              20μl<br>
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=3><b>electroelution Purification</b></font>
+
-
*[R0011 promoter]
+
-
<br><font color="#000000" size=2><b>Transformation of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
*[pSB1C3-acsAB]
+
-
:: Transform into E.coli
+
-
:: LB+CHLORAMPHENICOL
+
-
:: →37℃ for 14 hr
+
-
<br>
+
-
<br>
+
-
 
+
-
*[pSB1C3-acsCD]
+
-
:: Transform into E.coli
+
-
:: LB+CHLORAMPHENICOL
+
-
:: →37℃ for 14 hr
+
-
<br>
+
-
 
+
-
<br>
+
-
*[pSB1A3-acsCD]
+
-
:: Transform into E.coli
+
-
:: LB+Ampicillin
+
-
:: →37℃ for 14 hr
+
-
<br>
+
-
<br>
+
-
 
+
-
*[pSB1C3-CMCax-Ccp]
+
-
:: Transform into E.coli
+
-
:: LB+CHLORAMPHENICOL
+
-
:: →37℃ for 14 hr
+
-
<br>
+
-
<br>
+
-
 
+
-
<br><font color="#000000" size=2><b>Digestion of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
[R0011 promoter/EcoRⅠ+XbaⅠ]
+
-
<br>DNA           10μl<br>
+
-
<br>10×buffer        5μl<br>
+
-
<br>BSA           5μl<br>
+
-
<br>EcoRⅠ          1μl<br>
+
-
<br>XbaⅠ           1μl<br>
+
-
<br>ddH2O          28μl<br>
+
-
<br>____________________________
+
-
 
+
-
<br>total             50μl<br>
+
-
<br>→37℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>electroelution Purification</b></font>
+
-
*[R0011 promoter/EcoRⅠ+XbaⅠ]
+
-
 
+
-
<br><font color="#000000" size=3><b>2011.09.24</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Ligation of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
[pSB1C3-R0011]
+
-
<br>Vector          3μl<br>
+
-
<br>Insert          14μl<br>
+
-
<br>ligase buffer        2μl<br>
+
-
<br>ligase          1μl<br>
+
-
<br>ddH2O           -μl<br>
+
-
<br>________________________________
+
-
<br>
+
-
<br>total          20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
[R0011-acsAB]
+
-
<br>Vector          3μl<br>
+
-
<br>Insert          14μl<br>
+
-
<br>ligase buffer        2μl<br>
+
-
<br>ligase          1μl<br>
+
-
<br>ddH2O           -μl<br>
+
-
<br>________________________________
+
-
<br>
+
-
<br>total          20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
[R0011-acsCD]
+
-
<br>Vector          3μl<br>
+
-
<br>Insert          14μl<br>
+
-
<br>ligase buffer        2μl<br>
+
-
<br>ligase          1μl<br>
+
-
<br>ddH2O           -μl<br>
+
-
<br>________________________________
+
-
<br>
+
-
<br>total          20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
 
+
-
<br><font color="#000000" size=3><b>2011.09.24</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Digestion of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
[R0011-acsAB/EcoRⅠ+SpeⅠ]
+
-
<br>DNA           10μl<br>
+
-
<br>10×buffer        5μl<br>
+
-
<br>BSA           5μl<br>
+
-
<br>EcoRⅠ          1μl<br>
+
-
<br>SpeⅠ           1μl<br>
+
-
<br>ddH2O          28μl<br>
+
-
<br>____________________________
+
-
 
+
-
<br>total             50μl<br>
+
-
<br>→37℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
[R0011-acsCD/EcoRⅠ+SpeⅠ]
+
-
<br>DNA           10μl<br>
+
-
<br>10×buffer        5μl<br>
+
-
<br>BSA           5μl<br>
+
-
<br>EcoRⅠ          1μl<br>
+
-
<br>SpeⅠ           1μl<br>
+
-
<br>ddH2O          28μl<br>
+
-
<br>____________________________
+
-
<br>
+
-
<br>total             50μl<br>
+
-
<br>→37℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>electroelution Purification</b></font>
+
-
<br>
+
-
<br>
+
-
*[R0011-acsAB/EcoRⅠ+SpeⅠ]
+
-
 
+
-
*[R0011-acsCD/EcoRⅠ+SpeⅠ]
+
-
 
+
-
<br>
+
-
<br>
+
-
 
+
-
<br><font color="#000000" size=3><b>2011.09.25</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Ligation of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
[pSB1C3-R0011-acsAB]
+
-
<br>Vector          3μl<br>
+
-
<br>Insert          14μl<br>
+
-
<br>ligase buffer        2μl<br>
+
-
<br>ligase          1μl<br>
+
-
<br>ddH2O           -μl<br>
+
-
<br>________________________________
+
-
<br>
+
-
<br>total          20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br>
+
-
[pSB1C3-R0011-acsCD]
+
-
<br>Vector          3μl<br>
+
-
<br>Insert          14μl<br>
+
-
<br>ligase buffer        2μl<br>
+
-
<br>ligase          1μl<br>
+
-
<br>ddH2O           -μl<br>
+
-
<br>________________________________
+
-
<br>
+
-
<br>total          20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
 
+
-
<br><font color="#000000" size=3><b>2011.09.26</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Transformation of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
*[pSB1C3-R0011-acsAB]
+
-
:: Transform into E.coli
+
-
:: LB+CHLORAMPHENICOL
+
-
:: →37℃ for 14 hr
+
-
<br>
+
-
<br>
+
-
 
+
-
*[pSB1C3-R0011-acsCD]
+
-
:: Transform into E.coli
+
-
:: LB+CHLORAMPHENICOL
+
-
:: →37℃ for 14 hr
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Digestion of DNA</b></font>
+
-
<br>
+
-
 
+
-
[pSB1C3-R0011-acsCD/SpeⅠ+PstⅠ]
+
-
<br>DNA           10μl<br>
+
-
<br>10×buffer        5μl<br>
+
-
<br>BSA           5μl<br>
+
-
<br>SpeⅠ          1μl<br>
+
-
<br>PstⅠ           1μl<br>
+
-
<br>ddH2O          28μl<br>
+
-
<br>____________________________
+
-
 
+
-
<br>total             50μl<br>
+
-
<br>→37℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>electroelution Purification</b></font>
+
-
*[pSB1C3-R0011-acsCD/SpeⅠ+PstⅠ]
+
-
<br>
+
-
<br>
+
-
 
+
-
<br><font color="#000000" size=3><b>2011.09.27</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Ligation of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
[pSB1C3-R0011-acsCD-CMCax-Ccp]
+
-
<br>Vector          3μl<br>
+
-
<br>Insert          14μl<br>
+
-
<br>ligase buffer        2μl<br>
+
-
<br>ligase          1μl<br>
+
-
<br>ddH2O           -μl<br>
+
-
<br>________________________________
+
-
<br>
+
-
<br>total          20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Digestion of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
[pSB1C3-R0011-acsCD-CMCax-Ccp/PstⅠ+EcoRⅠ]
+
-
<br>DNA           10μl<br>
+
-
<br>10×buffer        5μl<br>
+
-
<br>BSA           5μl<br>
+
-
<br>PstⅠ          1μl<br>
+
-
<br>EcoRⅠ           1μl<br>
+
-
<br>ddH2O          28μl<br>
+
-
<br>____________________________
+
-
 
+
-
<br>total             50μl<br>
+
-
<br>→37℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>electroelution Purification</b></font>
+
-
*[pSB1C3-R0011-acsCD-CMCax-Ccp/PstⅠ+EcoRⅠ]
+
-
<br>
+
-
<br>
+
-
 
+
-
<br><font color="#000000" size=3><b>2011.09.28</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Ligation of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
[pSB1A3-R0011-acsCD-CMCax-Ccp]
+
-
<br>Vector          3μl<br>
+
-
<br>Insert          14μl<br>
+
-
<br>ligase buffer        2μl<br>
+
-
<br>ligase          1μl<br>
+
-
<br>ddH2O           -μl<br>
+
-
<br>________________________________
+
-
<br>
+
-
<br>total          20μl<br>
+
-
<br>→16℃ for 16 hr
+
-
<br>
+
-
<br>
+
-
<br><font color="#000000" size=3><b>2011.09.29</b></font>
+
-
<br>
+
-
<br><font color="#000000" size=2><b>Transformation of DNA</b></font>
+
-
<br>
+
-
<br>
+
-
*[pSB1C3-R0011-acsAB]
+
-
*[pSB1A3-R0011-acsAB-CMCax-Ccp]
+
-
:: Transform into E.coli
+
-
:: LB+Ampiclin+CHLORAMPHENICOL
+
-
:: →37℃ for 14 hr
+
<br>
<br>
<br>
<br>
<br>
<br>

Latest revision as of 02:56, 6 October 2011

Photopaper

Meeting Minutes


2011.02.24
Discussion:

  • Team organization
    caption
  • Brain storming
    • paper made by bacteria with add-ons such as colors, fragrance, etc.
    • "light up" the plants for replacing lamp posts.



2011.03.04
Discussion:

  • Team advisory
  • Brain storming
    • Add-ons: colorful cellulose which produced by bacteria such as beta-carotene
    • information exchange with iGEM 2009 Cambridge team




2011.03.14
Discussion:

  • Task Allocation
  • Brain storming
    • Avatar - "light up" the plants by transfecting symbiotic bacteria which cloned into fluorescence gene
    • Eco-friendly warmer - biotic thermal pad



2011.03.23
Discussion:

    • Project : paperia
    • Option 1 : Culture bacteria which has pigment gene
    • Option 2 : Cellulose-producing bacteria secrete pigment into the medium



2011.03.24
Discussion:

  • Exp. procedure:
    • cloning of cellulose gene’s CDS
    • the product should operate within E. coli.



2011.06.22
Discussion:

  • Due to some unforseen reason, the team decided to change their project.
  • New project: Biojenny

         -economical and humane way to produce paper in large quantities.
         -yeast to be our host



2011.07.01
Discussion:

  • Freeze > grin > genome DNA isolation > Cloning = silk protein gene



2011.07.09
Discussion:

  • the connections between 3 silk proteins : Fibl Fibh P25
  • major proteins : H-chain, L-chain, P25



2011.07.15
Discussion:

  • Due to limited resources and techniques required, the team decided to switch back to the previous project. Paper making !
  • However it would be modified to be more innovative and creative.



2011.07.18
Discussion:

  • Latest project : Photo paper
  • cyanobacteria is designed as the host, cellulose made up of the glucose produced by cyanobacteria could be one of the main attraction of the project.



2011.07.23
Discussion:

  • system modification to overcome the problems arises during preliminary round
  • Biobricks from Tokyo 2010 team will be utilized
    • regulator promoter in order to regulate the secretion of cellulose to solve the aggregation of the bacteria




2011.09.15

Genome miniprep
Gluconacetobacter hansenii



2011.09.18

Gel/PCR DNA extraction
Gluconacetobacter hansenii


Protocols




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