Team:Tsinghua/Notebook/22 July 2011
From 2011.igem.org
(Difference between revisions)
(SDS-PAGE-Pro-Cherry) |
|||
Line 2: | Line 2: | ||
No significant expression of desired protein(approximate molecular weight is 33KD) | No significant expression of desired protein(approximate molecular weight is 33KD) | ||
-------LI LI | -------LI LI | ||
+ | |||
+ | ==digest & ligate== | ||
+ | all the five parts |
Revision as of 09:17, 11 September 2011
2. SDS-page(10% gel, CBB(Coomassie brilliant blue) staining) No significant expression of desired protein(approximate molecular weight is 33KD)
LI LI
digest & ligate
all the five parts