"

From 2011.igem.org

Home

Team

Official Team Profile

Project

Parts Submitted to the Registry

Modeling

Notebook

Safety

Attributions

May M T W T F S S             [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/1_May_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/2_May_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/3_May_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/4_May_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/5_May_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/6_May_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/7_May_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/8_May_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/9_May_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/10_May_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/11_May_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/12_May_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/13_May_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/14_May_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/15_May_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/16_May_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/17_May_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/18_May_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/19_May_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/20_May_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/21_May_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/22_May_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/23_May_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/24_May_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/25_May_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/26_May_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/27_May_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/28_May_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/29_May_2011&action=edit 29] [http://2011.igem.org/Team:Toronto/Notebook/30_May_2011 30] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/31_May_2011&action=edit 31]

June M T W T F S S     [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/1_June_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/2_June_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/3_June_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/4_June_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/5_June_2011&action=edit 5] [http://2011.igem.org/Team:Toronto/Notebook/6_June_2011 6] [http://2011.igem.org/Team:Toronto/Notebook/7_June_2011 7] [http://2011.igem.org/Team:Toronto/Notebook/8_June_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/9_June_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/10_June_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/11_June_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/12_June_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/13_June_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/14_June_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/15_June_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/16_June_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/17_June_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/18_June_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/19_June_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/20_June_2011&action=edit 20] [http://2011.igem.org/Team:Toronto/Notebook/21_June_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/22_June_2011&action=edit 22] [http://2011.igem.org/Team:Toronto/Notebook/23_June_2011 23] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/24_June_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/25_June_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/26_June_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/27_June_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/28_June_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/29_June_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/30_June_2011&action=edit 30]

July M T W T F S S         [http://2011.igem.org/Team:Toronto/Notebook/1_July_2011 1] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/2_July_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/3_July_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/4_July_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/5_July_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/6_July_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/7_July_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/8_July_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/9_July_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/10_July_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/11_July_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/12_July_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/13_July_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/14_July_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/15_July_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/16_July_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/17_July_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/18_July_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/19_July_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/20_July_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/21_July_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/22_July_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/23_July_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/24_July_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/25_July_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/26_July_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/27_July_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/28_July_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/29_July_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/30_July_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/31_July_2011&action=edit 31]

August M T W T F S S [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/1_August_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/2_August_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/3_August_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/4_August_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/5_August_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/6_August_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/7_August_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/8_August_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/9_August_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/10_August_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/11_August_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/12_August_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/13_August_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/14_August_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/15_August_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/16_August_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/17_August_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/18_August_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/19_August_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/20_August_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/21_August_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/22_August_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/23_August_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/24_August_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/25_August_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/26_August_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/27_August_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/28_August_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/29_August_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/30_August_2011&action=edit 30] [http://2011.igem.org/Team:Toronto/Notebook/31_August_2011 31]

September M T W T F S S       [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/1_September_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/2_September_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/3_September_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/4_September_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/5_September_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/6_September_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/7_September_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/8_September_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/9_September_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/10_September_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/11_September_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/12_September_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/13_September_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/14_September_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/15_September_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/16_September_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/17_September_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/18_September_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/19_September_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/20_September_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/21_September_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/22_September_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/23_September_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/24_September_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/25_September_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/26_September_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/27_September_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/28_September_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/29_September_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/30_September_2011&action=edit 30]

October M T W T F S S           [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/1_October_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/2_October_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/3_October_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/4_October_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/5_October_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/6_October_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/7_October_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/8_October_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/9_October_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/10_October_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/11_October_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/12_October_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/13_October_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/14_October_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/15_October_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/16_October_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/17_October_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/18_October_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/19_October_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/20_October_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/21_October_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/22_October_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/23_October_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/24_October_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/25_October_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/26_October_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/27_October_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/28_October_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/29_October_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/30_October_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Toronto/Notebook/31_October_2011&action=edit 31]

J23100+B0034+C0012+J04500 construct samples 22 / 24 / 14 were digested with SpeI and PstI for the insertion of His-5 MMS6.

MMS6 was also PCR amplified for this insertion. A duplicate reaction was performed in case of downstream reactions failed

A gel was run to confirm the digestion and that the PCR reaction produced only one band.

<insert image of gel Aug 31 (1)> --> Suraj has chart of lane contents.

From the gel it was confirmed that the PCR had no side reactions and that the J23100 construct was cut

The PCR products were then purified using the Geneaid Kit

PCR reaction 1 (p) when eluted in 50ul of elution buffer had a concentration of 23.5ng/ul'

PCR reaction 1 (g) when eluted in 50ul of elution buffer had a concentration of 41.4ng/ul'

The J23100 digested constructs were then Gel purified. resulting in a concentration of - add when completed

An overnight ligation was then performed.