Team:Tokyo Tech/Projects/Urea-cooler/data

From 2011.igem.org

(Difference between revisions)
Line 306: Line 306:
<h1> Urea-cooler Assay data </h1>
<h1> Urea-cooler Assay data </h1>
<h2 id="samples">1. Characterization of rocF and Arg box</h2>
<h2 id="samples">1. Characterization of rocF and Arg box</h2>
-
<h3 id="Materials for 1C3">Materials</h3>
+
<h3 id="Materials for 1C3">1.1 Materials</h3>
<p>Expression plasmids used in this study are shown in Table 1. <br />
<p>Expression plasmids used in this study are shown in Table 1. <br />
<table border="1">
<table border="1">
Line 336: Line 336:
Strain MG1655 was transformed with either mock, tocF or rocF + Arg. As shown in Table 1, rocF gene was introduced on pSB3K3 and Arg boxes were introduced on pSB6A1.
Strain MG1655 was transformed with either mock, tocF or rocF + Arg. As shown in Table 1, rocF gene was introduced on pSB3K3 and Arg boxes were introduced on pSB6A1.
</p>
</p>
-
<h3 id="Methods for 1C3">Methods</h3>
+
<h3 id="Methods for 1C3">1.2 Methods</h3>
         <p>Methods for this study are described <a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method">here</a>
         <p>Methods for this study are described <a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method">here</a>
         </p>
         </p>

Revision as of 16:27, 26 October 2011

Urea-cooler Assay data

1. Characterization of rocF and Arg box

1.1 Materials

Expression plasmids used in this study are shown in Table 1.

TABLE 1. Expression plasmids used for Charcterization of rocF and Arg box
designation pSB3K3 pSB6A1
mock PlacIQ Alcohol-dehydrogenase(promoter-less)
rocF Ptrc-rocF Alcohol-dehydrogenase(promoter-less)
rocF+Arg box Ptrc-rocF Arg box
Strain MG1655 was transformed with either mock, tocF or rocF + Arg. As shown in Table 1, rocF gene was introduced on pSB3K3 and Arg boxes were introduced on pSB6A1.

1.2 Methods

Methods for this study are described here

Return to Page Top