Team:Tokyo Tech/Projects/Urea-cooler/data

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<table border="1">
<table border="1">
<caption>TABLE 1. Expression plasmids used for Charcterization of rocF and Arg box</caption>
<caption>TABLE 1. Expression plasmids used for Charcterization of rocF and Arg box</caption>
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<tr>
+
-
<th></th>
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-
<th>vector</th>
+
-
<th><i>rocF</i></th>
+
-
+
-
</tr>
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<tr>
<tr>
<td>designation</td>
<td>designation</td>
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<td>PlacIQ</td>
<td>PlacIQ</td>
<td>Alcohol-dehydrogenase(promoter-less)</td>
<td>Alcohol-dehydrogenase(promoter-less)</td>
-
<td style="text-align:center;">+</td>
 
</tr>
</tr>
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                                  <tr>
 +
<td><i>rocF</i></td>
 +
<td>Ptrc-<i>rocF</i></td>
 +
<td>Alcohol-dehydrogenase(promoter-less)</td>
 +
</tr>
 +
                                    <tr>
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<td><i>rocF</i>+Arg box</td>
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<td>Ptrc-<i>rocF</i></td>
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<td>Arg box</td>
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</tr>
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</table>
</table>

Revision as of 16:15, 26 October 2011

Urea-cooler Assay data

1. Characterization of rocF and Arg box

Materials

Expression plasmids used in this study are shown in Table 1.

TABLE 1. Expression plasmids used for Charcterization of rocF and Arg box
designation pSB3K3 pSB6A1
mock PlacIQ Alcohol-dehydrogenase(promoter-less)
rocF Ptrc-rocF Alcohol-dehydrogenase(promoter-less)
rocF+Arg box Ptrc-rocF Arg box
Strain MG1655 was transformed with either mock, tocF or rocF + Arg.

Methods

Methods for this study are described here

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