"
Team:Tokyo Tech/DataPage.htm
From 2011.igem.org
(Difference between revisions)
| (38 intermediate revisions not shown) | |||
| Line 34: | Line 34: | ||
list-style: none; | list-style: none; | ||
float: left; | float: left; | ||
| - | height: | + | height: 30px; |
width: 945px; | width: 945px; | ||
} | } | ||
| Line 55: | Line 55: | ||
display: none; | display: none; | ||
position: absolute; | position: absolute; | ||
| - | font-size: | + | font-size: 16px; |
opacity: 0.8; | opacity: 0.8; | ||
list-style: none; | list-style: none; | ||
| Line 79: | Line 79: | ||
background-color: #EFEBEC; | background-color: #EFEBEC; | ||
color: #000000; | color: #000000; | ||
| + | font-size: 13px; | ||
} | } | ||
| Line 86: | Line 87: | ||
left: 0; | left: 0; | ||
clear: both; | clear: both; | ||
| - | height: | + | height: 190px; |
} | } | ||
.main | .main | ||
| Line 114: | Line 115: | ||
{ | { | ||
text-indent: 1em; | text-indent: 1em; | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
} | } | ||
| Line 126: | Line 122: | ||
} | } | ||
| - | + | h4 | |
{ | { | ||
| - | + | text-indent: 3em; | |
} | } | ||
| - | + | h5 | |
{ | { | ||
| - | text-indent: | + | text-indent: 4em; |
} | } | ||
| - | + | ||
| - | + | h6 | |
{ | { | ||
| - | + | text-indent: 5em; | |
} | } | ||
| Line 160: | Line 156: | ||
.name | .name | ||
| + | { | ||
| + | font-style: italic; | ||
| + | } | ||
| + | |||
| + | .gene | ||
{ | { | ||
font-style: italic; | font-style: italic; | ||
| Line 166: | Line 167: | ||
p | p | ||
{ | { | ||
| - | text-indent: | + | text-indent: 2em; |
} | } | ||
| Line 214: | Line 215: | ||
} | } | ||
} | } | ||
| + | |||
| + | function getElementsByClass() { | ||
| + | var classElements = new Array(); | ||
| + | var allElements = document.getElementsByTagName("*"); | ||
| + | for (i = 0 ; i < allElements.length; i++) { | ||
| + | if (allElements[i].className == 'firstHeading') { | ||
| + | allElements[i].style.display = 'none'; | ||
| + | // window.alert('発見。'); | ||
| + | } | ||
| + | } | ||
| + | } | ||
| - | + | window.onload = function() { | |
| + | getElementsByClass(); | ||
var menu = window.document.getElementById('TopMenu'); | var menu = window.document.getElementById('TopMenu'); | ||
if(menu==null) | if(menu==null) | ||
| Line 248: | Line 261: | ||
<!-- top box --> | <!-- top box --> | ||
<div class="top"> | <div class="top"> | ||
| - | < | + | <object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,0,0" width="965" height="150" id="Yourfilename" align=""> |
| - | < | + | <param name="movie" value="https://static.igem.org/mediawiki/2011/4/4d/Header.swf"> |
| + | <param name="quality" value="high"> | ||
| + | <param name="bgcolor" value="#FFFFFF"> | ||
| + | <embed src="https://static.igem.org/mediawiki/2011/4/4d/Header.swf" quality="high" bgcolor="#FFFFFF" width="965" height="150" name="Yourfilename" align="" type="application/x-shockwave-flash" pluginspage="http://www.macromedia.com/go/getflashplayer"> | ||
| + | </embed> | ||
| + | </object> | ||
<!-- list of top menu --> | <!-- list of top menu --> | ||
<div id="navigation"> | <div id="navigation"> | ||
| Line 256: | Line 274: | ||
<li id="menu_Project"> | <li id="menu_Project"> | ||
| - | + | Project | |
<ul> | <ul> | ||
| - | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/index.htm">RPS- | + | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/index.htm">RPS-Game</a></li> |
| - | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm"> | + | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm">Make it Rain</a></li> |
| - | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm"> | + | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm">Urea Coolers</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| Line 269: | Line 287: | ||
Modeling | Modeling | ||
<ul> | <ul> | ||
| - | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Modeling/RPS-game/RPS-game">RPS- | + | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Modeling/RPS-game/RPS-game">RPS-Game</li> |
| - | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Modeling/Urea-cooler/urea-cooler"> | + | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Modeling/Urea-cooler/urea-cooler">Urea Coolers</li> |
</ul> | </ul> | ||
</li> | </li> | ||
| - | <li id=" | + | <li id="menu_Human Practice"><a href="https://2011.igem.org/Team:Tokyo_Tech/HumanPractice.htm">Human Practice</a></li> |
<li id="menu_Sitemap"><a href="https://2011.igem.org/Team:Tokyo_Tech/sitemap.htm">Sitemap</a></li> | <li id="menu_Sitemap"><a href="https://2011.igem.org/Team:Tokyo_Tech/sitemap.htm">Sitemap</a></li> | ||
<li id="menu_Extra"> | <li id="menu_Extra"> | ||
| - | + | More | |
| - | <ul> | + | <ul style="width:210px;"> |
<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Safety">Safety</a></li> | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Safety">Safety</a></li> | ||
<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Attribution_and_Contributions.htm">Attribution and Contributions</a></li> | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Attribution_and_Contributions.htm">Attribution and Contributions</a></li> | ||
<li><a href="https://2011.igem.org/Team:Tokyo_Tech/notebook">NoteBook</a></li> | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/notebook">NoteBook</a></li> | ||
<li><a href="https://2011.igem.org/Team:Tokyo_Tech/team">Team</a></li> | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/team">Team</a></li> | ||
| - | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Sponsers.htm"> | + | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Sponsers.htm">Sponsors</a></li> |
<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Collaboration.htm">Collaboration</a></li> | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Collaboration.htm">Collaboration</a></li> | ||
</ul> | </ul> | ||
| Line 299: | Line 317: | ||
<!-- left menu list --> | <!-- left menu list --> | ||
| - | <div style="min-height: | + | <div style="min-height: 2000px; float: left;"> |
<div id="LeftMenu"> | <div id="LeftMenu"> | ||
<!--list of page menu: DO NOT WRITE LINKS NOT WRITTEN IN THIS PAGE --> | <!--list of page menu: DO NOT WRITE LINKS NOT WRITTEN IN THIS PAGE --> | ||
<ul> | <ul> | ||
| - | <li><a href="#system">How our system works</a></li> | + | <li><a href="#system">1. How our system works</a></li> |
| - | <li><a href="#new_parts">Data For Our Favorite New Parts</a></li> | + | <li><a href="#new_parts">2. Data For Our Favorite New Parts</a></li> |
| - | <li><a href="#improve">Data For Pre-existing Parts</a></li> | + | <li><a href="#improve">3. Data For Pre-existing Parts</a></li> |
| - | <li><a href="#chared">We’ve Also Characterized the Following Parts</a></li> | + | <li><a href="#chared">4. We’ve Also Characterized the Following Parts</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 313: | Line 331: | ||
<!-- main contents --> | <!-- main contents --> | ||
<div class="main"> | <div class="main"> | ||
| - | <!-- ############ | + | <!-- ############ Write main contents here ############### --> |
<!-- page title --> | <!-- page title --> | ||
| Line 319: | Line 337: | ||
<h1> Data page </h1> | <h1> Data page </h1> | ||
| - | + | <p> | |
| - | + | This page shows a list of all the parts that | |
| - | + | we have made or used in the project. | |
| - | + | Click on the link for each part to see | |
| - | + | more details about that part on the | |
| + | Registry of Standard Biological Parts. | ||
| + | For a brief overview of our main results, | ||
| + | please have a look at our Main Results page. | ||
| + | </p> | ||
| - | + | <h2 id="system">1. How our system Works</h2> | |
| - | + | <img src="https://static.igem.org/mediawiki/2011/2/20/Allinone1.png" alt="allinone" width="800px" /> | |
| - | + | <h2 id="new_parts">2. Data For Our Favorite New Parts</h2> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | <ul> | |
| - | + | <li> | |
| - | + | <a href="http://partsregistry.org/Part:BBa_K649001">BBa_K649001</a> | |
| - | + | -- <b>GFP regulated by 3OC12-HSL and LasR</b><br /> | |
| - | + | in the presence of 3OC12-HSL, lasI promoter can be induced to express a marker gene(<span class="gene">gfp</span>). | |
| - | + | </li> | |
| - | + | ||
| - | + | <li> | |
| - | + | <a href="http://partsregistry.org/Part:BBa_K649202">BBa_K649202</a> | |
| - | + | -- <b>PlacIQ-<i>lox71</i>-rbs-<span class="gene">rfp</span>-<i>lox66</i>-rbs-<span class="gene">gfp</span></b><br /> | |
| - | + | in the presence of Cre, a marker gene (<span class="gene">rfp</span>) between <i>lox71</i> and <i>lox66</i> is knockout, and a marker gene (<span class="gene">gfp</span>) is expressed. | |
| - | + | </li> | |
| - | + | ||
| - | + | <li> | |
| - | + | <a href="http://partsregistry.org/Part:BBa_K649301">BBa_K649301</a> | |
| - | + | -- <b>Ptrc-rbs-<span class="gene">rocF</span></b><br /> | |
| - | + | because arginase is constitutively expressed, the expression level of urea in <span class="name">E. coli</span> transformed with BBa_K649301 was higher than geneless <span class="name">E. coli</span>. | |
| - | + | </li> | |
| + | </ul> | ||
| - | + | <h2 id="improve">3. Data For Pre-existing Parts</h2> | |
| - | + | <ul> | |
| + | <li> | ||
| + | <a href="http://partsregistry.org/Part:BBa_J64010:Experience">BBa_J64010:Experience</a> | ||
| + | -- <b>lasI promoter</b>, BBa_J64010 (Voigt Lab, 2007)<br /> | ||
| + | fluorescence intensity of PlasI (BBa_J64010) -<span class="gene">gfp</span> did not change before and after 3OC12-HSL induction. | ||
| + | </li> | ||
| + | |||
| + | <li> | ||
| + | <a href="http://partsregistry.org/Part:BBa_I751101:Experience">BBa_I751101:Experience</a> | ||
| + | -- <b>J540140 dPr + hybrid promoter (Plux-lac)</b>, BBa_I751101 (Tokyo Tech, 2007)<br /> | ||
| + | fluorescence intensity of BBa_I751101 was increased by both 3OC6-HSL induction and IPTG induction. | ||
| + | </li> | ||
| + | |||
| + | <li> | ||
| + | <a href="http://partsregistry.org/Part:BBa_K117002:Experience">BBa_K117002:Experience</a> | ||
| + | -- <b>LsrA promoter (indirectly activated by AI-2)</b>, BBa_K117002 (NTU-Singapore, 2008)<br /> | ||
| + | in the absence of LsrR, fluorescence intensity of PlsrA (BBa_K117002) -<span class="gene">gfp</span> was lower than that of promoterless negative control. | ||
| + | </li> | ||
| + | </ul> | ||
| - | + | <h2 id="chared">4. We’ve Also Characterized the Following Parts</h2> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | <ul> | |
| - | + | <li> | |
| - | + | <a href="http://partsregistry.org/Part:BBa_K649104">BBa_K649104</a> | |
| - | + | -- <b>PlsrA-rbs-<span class="gene">gfp</span></b><br /> | |
| - | + | in the absence of LsrR, fluorescence intensity of BBa_K649104 was much higher than promoterless-<span class="gene">gfp</span> (negative control). | |
| - | + | </li> | |
| - | + | ||
| - | + | <li> | |
| - | + | <a href="http://partsregistry.org/Part:BBa_K649105">BBa_K649105</a> | |
| - | + | -- <b>PlsrA-rbs-<span class="gene">gfp</span>-TT-PlsrR-rbs-<span class="gene">lsrR</span></b><br /> | |
| - | + | PlsrA (BBa_K649100) was repressed by LsrR and fluorescence intensity of BBa-K649105 decreased 3-fold. | |
| - | + | </li> | |
| - | + | ||
| - | + | <li> | |
| - | + | <a href="http://partsregistry.org/Part:BBa_K649200">BBa_K649200</a> | |
| - | + | -- <b>PlacIQ-<i>lox2272</i>-rbs-<span class="gene">gfp</span>-<i>lox2272</i></b><br /> | |
| - | + | in the presence of Cre, the sequence between two <i>lox2272</i> is knockout. | |
| - | + | </li> | |
| - | + | <li> | |
| - | + | <a href="http://partsregistry.org/Part:BBa_K649201">BBa_K649201</a> | |
| - | + | -- <b>PlacIQ-<i>lox2272</i>-rbs-<span class="gene">rfp</span>-<i>lox2272</i>-rbs-<span class="gene">gfp</span></b><br /> | |
| - | + | in the presence of Cre, a marker gene (<span class="gene">rfp</span>) between two <i>lox2272</i> is knockout, and a marker gene (<span class="gene">gfp</span>) is expressed. | |
| - | + | </li> | |
| - | + | <li> | |
| - | + | <a href="http://partsregistry.org/Part:BBa_K649303">BBa_K649303</a> | |
| - | + | -- <b>PlacIQ-rbs-<i>ispS</i></b><br /> | |
| - | + | we confirmed that <span class="name">E. coli</span> introduced <i>ispS</i> produced isoprene, by means of using electron-ionization Gas Chromatography-Mass Spectrometry equipment. | |
| - | + | </li> | |
| - | + | <li> | |
| - | + | <a href="http://partsregistry.org/Part:BBa_K649401">BBa_K649401</a> | |
| - | + | -- <b>Arg box</b><br /> | |
| - | + | because Arg Box is the arginine operator which the arginine repressor can bind to, the expression level of urea in <span class="name">E. coli</span> transformed with BBa_K649401 was higher than mock <span class="name">E. coli</span>. | |
| - | + | </li> | |
| - | + | <li> | |
| - | + | <a href="http://partsregistry.org/Part:BBa_K649402">BBa_K649402</a> | |
| - | + | -- <b>Ptrc-rbs-<span class="gene">rocF</span>-Arg box</b><br /> | |
| - | + | because we transformed <span class="name">E. coli</span> with BBa_K649402 on low copy plasmid, Arg box did not replicated adequately and we could not confirm whether Arg box was working or not. | |
| - | + | </li> | |
| - | + | ||
| - | + | </ul> | |
| - | + | ||
| - | + | ||
| - | + | ||
| Line 427: | Line 443: | ||
<!-- end of main contents --> | <!-- end of main contents --> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
</p> | </p> | ||
<!-- end of under contents --> | <!-- end of under contents --> | ||
| + | |||
| + | <div style="bottom: 0;left: 30; position:fixed; background-color: #000000; opacity:0.8;"> | ||
| + | <a onclick="scrollTo(0,0);return false;" onfocus="this.blur();" style="color:#FFFFFF;">Return to Page Top</a> | ||
| + | </div> | ||
<!-- DO NOT WRITE UNDER HERE --> | <!-- DO NOT WRITE UNDER HERE --> | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 13:15, 28 October 2011
Data page
This page shows a list of all the parts that we have made or used in the project. Click on the link for each part to see more details about that part on the Registry of Standard Biological Parts. For a brief overview of our main results, please have a look at our Main Results page.
1. How our system Works
2. Data For Our Favorite New Parts
-
BBa_K649001
-- GFP regulated by 3OC12-HSL and LasR
in the presence of 3OC12-HSL, lasI promoter can be induced to express a marker gene(gfp). -
BBa_K649202
-- PlacIQ-lox71-rbs-rfp-lox66-rbs-gfp
in the presence of Cre, a marker gene (rfp) between lox71 and lox66 is knockout, and a marker gene (gfp) is expressed. -
BBa_K649301
-- Ptrc-rbs-rocF
because arginase is constitutively expressed, the expression level of urea in E. coli transformed with BBa_K649301 was higher than geneless E. coli.
3. Data For Pre-existing Parts
-
BBa_J64010:Experience
-- lasI promoter, BBa_J64010 (Voigt Lab, 2007)
fluorescence intensity of PlasI (BBa_J64010) -gfp did not change before and after 3OC12-HSL induction. -
BBa_I751101:Experience
-- J540140 dPr + hybrid promoter (Plux-lac), BBa_I751101 (Tokyo Tech, 2007)
fluorescence intensity of BBa_I751101 was increased by both 3OC6-HSL induction and IPTG induction. -
BBa_K117002:Experience
-- LsrA promoter (indirectly activated by AI-2), BBa_K117002 (NTU-Singapore, 2008)
in the absence of LsrR, fluorescence intensity of PlsrA (BBa_K117002) -gfp was lower than that of promoterless negative control.
4. We’ve Also Characterized the Following Parts
-
BBa_K649104
-- PlsrA-rbs-gfp
in the absence of LsrR, fluorescence intensity of BBa_K649104 was much higher than promoterless-gfp (negative control). -
BBa_K649105
-- PlsrA-rbs-gfp-TT-PlsrR-rbs-lsrR
PlsrA (BBa_K649100) was repressed by LsrR and fluorescence intensity of BBa-K649105 decreased 3-fold. -
BBa_K649200
-- PlacIQ-lox2272-rbs-gfp-lox2272
in the presence of Cre, the sequence between two lox2272 is knockout. -
BBa_K649201
-- PlacIQ-lox2272-rbs-rfp-lox2272-rbs-gfp
in the presence of Cre, a marker gene (rfp) between two lox2272 is knockout, and a marker gene (gfp) is expressed. -
BBa_K649303
-- PlacIQ-rbs-ispS
we confirmed that E. coli introduced ispS produced isoprene, by means of using electron-ionization Gas Chromatography-Mass Spectrometry equipment. -
BBa_K649401
-- Arg box
because Arg Box is the arginine operator which the arginine repressor can bind to, the expression level of urea in E. coli transformed with BBa_K649401 was higher than mock E. coli. -
BBa_K649402
-- Ptrc-rbs-rocF-Arg box
because we transformed E. coli with BBa_K649402 on low copy plasmid, Arg box did not replicated adequately and we could not confirm whether Arg box was working or not.
