Team:Tokyo Tech

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<li><a href="#manabi"> Human Practice</a></li>
<li><a href="#manabi"> Human Practice</a></li>
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When summer comes it brings vacations, so students have plenty time to have fun.  
When summer comes it brings vacations, so students have plenty time to have fun.  
But summer means hot weather! In light of these circumstances,  
But summer means hot weather! In light of these circumstances,  
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we designed a game that can be played between<span class="name"> E.coli</span> and humans,
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we designed a game that can be played between<span class="name"> E.coli</span> and humans.
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and that the winner of the game can get refreshing prizes!  
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The winner of the game can get refreshing prizes!  
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Dear students, let us introduce you to the first human-bacteria Rock-Paper-Scissors game!  
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Dear students and judges, let us introduce you to the first human-bacteria Rock-Paper-Scissors game!  
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Win and get a rain shower, plus a “urea cooler”!
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Win and get a rain shower and a &ldquo;urea cooler&rdquo;!
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In our set of six signaling molecules, humans use IPTG,  
In our set of six signaling molecules, humans use IPTG,  
aTc and salicylate, and <span class="name">E. coli</span> uses  
aTc and salicylate, and <span class="name">E. coli</span> uses  
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3O-C6-HSL, 3O-C12-HSL and AI-2. In each case,  
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3OC6-HSL, 3OC12-HSL and AI-2. In each case,  
these signaling molecules correspond to rock, paper and scissors respectively.
these signaling molecules correspond to rock, paper and scissors respectively.
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every time it plays. Importantly, we have to design the opponent  
every time it plays. Importantly, we have to design the opponent  
<span class="name">E. coli</span> to be able to choose its signal  
<span class="name">E. coli</span> to be able to choose its signal  
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randomly for a fare game, because <span class="name">E. coli</span>  
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randomly for a fair game, because <span class="name">E. coli</span>  
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will continue to lose if <span class="name">E. coli</span> always  
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will continue to lose if it always produces the same signaling
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produce same signaling molecule. In a view of these needs,  
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molecule. In a view of these needs, we designed three randomizers that
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we designed three randomizers that satisfy the conditions for the game.
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satisfy the conditions for the game:Single Colony Isolation, Survival  
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Single Colony Isolation, Survival of single strain and Conditional Knockout.  
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of One Strain and Conditional Knockout.  
<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/index.htm">(see more...)</a>
<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/index.htm">(see more...)</a>
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<p>
Playing RPS with <span class="name">E. coli</span> during summer was fun, but,  
Playing RPS with <span class="name">E. coli</span> during summer was fun, but,  
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even if humans won, pleasures did not last long since we soon returned to  
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even if humans won, joy did not last long since we soon returned to  
complaining about the hot weather. As a prize for humans who win in our RPS game,  
complaining about the hot weather. As a prize for humans who win in our RPS game,  
we designed an <span class="name">E. coli</span> that can make it rain,  
we designed an <span class="name">E. coli</span> that can make it rain,  
making the hot summer more fun and refreshing (let alone applications in agriculture).  
making the hot summer more fun and refreshing (let alone applications in agriculture).  
To make it rain, we constructed an isoprene synthetizing <span class="name">E. coli</span>.  
To make it rain, we constructed an isoprene synthetizing <span class="name">E. coli</span>.  
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Photo-oxidized isoprene acts as a condensation nucleus(Colin D. O‘Dowd <i> et al</i>., 2002, Nature),  
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Photo-oxidized isoprene acts as a condensation nucleus(Leonardo Silva Santos <i>et al</i>.,2006),  
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which might cause rain, even though it is present in very low concentrations.
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which might cause rain, even in very low concentrations.
<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm">(see more...)</a>
<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm">(see more...)</a>
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To analyze how we obtain even more urea from our <span class="name">E. coli</span>,  
To analyze how we obtain even more urea from our <span class="name">E. coli</span>,  
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elementary flux analysis (Schuste<i>et al.</i> 2000) identified metabolic routes
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we used elementary flux analysis (Schuste <i>et al.</i>, 2000) to identify
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that are both stoichiometrically and thermodynamically feasible for a group of enzymes.  
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Making use of it we can tell which substrates and pathways are the best for obtaining  
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metabolic routes that are both stoichiometrically and thermodynamically  
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more urea.<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm">(see more...)</a>
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feasible for a group of enzymes. Making use of it we can tell which  
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                substrates and pathways are the best for obtaining  
 +
               
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                more urea.<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm">(see more...)</a>
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<h2 id="manabi"> Human Practice </h2>
<h2 id="manabi"> Human Practice </h2>
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<img src="https://static.igem.org/mediawiki/2011/2/22/Manabi-ppt2.png" alt="Creating Perception" width="480px" class="fig" />
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<img src="https://static.igem.org/mediawiki/2011/2/25/Creating_Perception_1.png" alt="Creating Perception" width="480px" class="fig" />
<p>
<p>
We love Synthetic Biology, and to share our passion with other people,  
We love Synthetic Biology, and to share our passion with other people,  
we did educational activities as part of our human practices.  
we did educational activities as part of our human practices.  
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We would like to spread what is made and aimed by synthetic biology and iGEM.  
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We would like to spread the word about what is made and aimed by synthetic
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For this event, we created “iGEM Card Game”, posters and questionnaire.
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biology and iGEM. To do so, we created the &ldquo;iGEM Card Game&rdquo; and several 
 +
                posters. We also measured our impact through a survey.
<a href="https://2011.igem.org/Team:Tokyo_Tech/HumanPractice.htm">(see more...)</a>
<a href="https://2011.igem.org/Team:Tokyo_Tech/HumanPractice.htm">(see more...)</a>
</p>
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Latest revision as of 16:44, 28 October 2011


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iGEM Tokyo Tech 2011 Team

Overview: Cool down in summer with our Rock-Paper-Scissors Game

Project Overview

When summer comes it brings vacations, so students have plenty time to have fun. But summer means hot weather! In light of these circumstances, we designed a game that can be played between E.coli and humans. The winner of the game can get refreshing prizes! Dear students and judges, let us introduce you to the first human-bacteria Rock-Paper-Scissors game! Win and get a rain shower and a “urea cooler”!

Rock-Paper-Scissors (RPS) Game

RPS

So, how to play RPS with a handless bacteria? Instead of hands, we use different signaling molecules corresponding either to rock, paper or scissors. In our set of six signaling molecules, humans use IPTG, aTc and salicylate, and E. coli uses 3OC6-HSL, 3OC12-HSL and AI-2. In each case, these signaling molecules correspond to rock, paper and scissors respectively.

The next step is to design a way to know who wins the RPS game. That is when the E. coli judge team comes in! Each judge has an AND-gate promoter which produces a single output based on two inputs, human side and E.coli side signaling molecules. Since we want the output to be visible, we use either GFP, RFP or CFP to indicate whether humans win, lose or it is a tie, respectively.

Lastly, we need to make sure E. coli follows the rules of the game by synthetizing only one signaling molecule every time it plays. Importantly, we have to design the opponent E. coli to be able to choose its signal randomly for a fair game, because E. coli will continue to lose if it always produces the same signaling molecule. In a view of these needs, we designed three randomizers that satisfy the conditions for the game:Single Colony Isolation, Survival of One Strain and Conditional Knockout. (see more...)

Make it Rain

Raining

Playing RPS with E. coli during summer was fun, but, even if humans won, joy did not last long since we soon returned to complaining about the hot weather. As a prize for humans who win in our RPS game, we designed an E. coli that can make it rain, making the hot summer more fun and refreshing (let alone applications in agriculture). To make it rain, we constructed an isoprene synthetizing E. coli. Photo-oxidized isoprene acts as a condensation nucleus(Leonardo Silva Santos et al.,2006), which might cause rain, even in very low concentrations. (see more...)

Urea Coolers

Urea cooler

Coolers can be made by adding urea to water, since dissolving urea in water is an endothermic reaction (-57.8 cal/g). We can make E.coli synthetize urea just by introducing a gene encoding arginase!

To analyze how we obtain even more urea from our E. coli, we used elementary flux analysis (Schuste et al., 2000) to identify metabolic routes that are both stoichiometrically and thermodynamically feasible for a group of enzymes. Making use of it we can tell which substrates and pathways are the best for obtaining more urea.(see more...)

Human Practice

Creating Perception

We love Synthetic Biology, and to share our passion with other people, we did educational activities as part of our human practices. We would like to spread the word about what is made and aimed by synthetic biology and iGEM. To do so, we created the “iGEM Card Game” and several posters. We also measured our impact through a survey. (see more...)

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