Team:Tokyo Tech

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Project
<ul>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/index.htm">RPS-game</a></li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/index.htm">RPS-Game</a></li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/makeing-rain/index.htm">rain</a></li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm">Make it Rain</a></li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm">urea cooler</a></li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm">Urea Coolers</a></li>
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Modeling
<ul>
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<li>RPS-game</li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Modeling/RPS-game/RPS-game">RPS-Game</li>
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<li>rain</li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Modeling/Urea-cooler/urea-cooler">Urea Coolers</li>
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<li>urea cooler</li>
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</ul>
</ul>
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<li id="menu_HumanPractice"><a href="https://2011.igem.org/Team:Tokyo_Tech/HumanPractice.htm">Human Practice</a></li>
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<li id="menu_Human Practice"><a href="https://2011.igem.org/Team:Tokyo_Tech/HumanPractice.htm">Human Practice</a></li>
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<li id="menu_Experience"><a href"https://2011.igem.org/Team::Tokyo_Tech/experience.htm>Experience</a></li>
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More
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Safety">Safety</a></li>
<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Safety">Safety</a></li>
<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Attribution_and_Contributions.htm">Attribution and Contributions</a></li>
<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Attribution_and_Contributions.htm">Attribution and Contributions</a></li>
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<li>NoteBook</li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/notebook">NoteBook</a></li>
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<li>Team</li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/team">Team</a></li>
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<li>Sponsers</li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Sponsers.htm">Sponsors</a></li>
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<li>Collaboration</li>
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<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Collaboration.htm">Collaboration</a></li>
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<ul>
<ul>
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<li><a href="#Overall">1. Cool down in summer with our Rock, Paper and Scissors Game</a></li>
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<li><a href="#Overall"> Overview: Cool down with our RPS Game</a></li>
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<li><a href="#RPS">2. Rock, Paper and Scissors (RPS) game</a></li>
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<li><a href="#RPS"> Rock-Paper-Scissors (RPS) Game</a></li>
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<li><a href="#rain">3. Make it rain</a></li>
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<li><a href="#rain"> Make it rain</a></li>
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<li><a href="#urea">4. Urea cooler</a></li>
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<li><a href="#urea"> Urea Coolers</a></li>
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<li><a href="#manabi"> Human Practice</a></li>
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<h1> iGEM Tokyo Tech 2011 Team </h1>
<h1> iGEM Tokyo Tech 2011 Team </h1>
-
<h2 id="Overall">1. Cool down in summer with our Rock, Paper and Scissors Game</h2>
+
<h2 id="Overall"> Overview: Cool down in summer with our Rock-Paper-Scissors Game</h2>
 +
<img src="https://static.igem.org/mediawiki/2011/2/24/Happy-rps.png" width="408px" alt="Project Overview" style="float:right;" />
<p>
<p>
-
When summer comes it brings vacations, so students have plenty time to have fun. But summer means hot weather! At times we wish we could just make it rain to refresh ourselves. In light of these circumstances, and given the opportunity to do experiments with E. coli through our iGEM project activity, we designed a game that can be played between E.coli and humans. Still more, the winner of the game can get refreshing prizes! Dear students, let us introduce you to the first human-bacteria Rock, Paper and Scissors game! Win and get a rain shower, plus a “urea cooler”!<br />
+
When summer comes it brings vacations, so students have plenty time to have fun.  
-
<img src="https://static.igem.org/mediawiki/2011/a/a8/TokyoTech_home_fig1.png" alt="Three projects" style="float:none;align:center;" />
+
But summer means hot weather! In light of these circumstances,  
 +
we designed a game that can be played between<span class="name"> E.coli</span> and humans.  
 +
The winner of the game can get refreshing prizes!  
 +
Dear students and judges, let us introduce you to the first human-bacteria Rock-Paper-Scissors game!  
 +
Win and get a rain shower and a &ldquo;urea cooler&rdquo;!
</p>
</p>
   
   
-
<h2 id="RPS">2. Rock, Paper and Scissors (RPS) game</h2>
+
<h2 id="RPS"> Rock-Paper-Scissors (RPS) Game </h2>
 +
<img src="https://static.igem.org/mediawiki/2011/1/10/TokyoTech_home_fig2.png" alt="RPS" width="480px" class="fig" />
<p>
<p>
-
So, how to play RPS with a handless bacteria? Instead of hands, we designed our game so that we could use different signaling molecules corresponding either to rock, paper or scissors. In our set of six signaling molecules, humans use IPTG, aTc and salicylate. E. coli uses 3O-C6-HSL, 3O-C12-HSL and AI-2. In each case, these signaling molecules correspond to rock, paper and scissors respectively.  
+
So, how to play RPS with a handless bacteria?  
 +
Instead of hands, we use different signaling molecules corresponding  
 +
either to rock, paper or scissors.  
 +
In our set of six signaling molecules, humans use IPTG,  
 +
aTc and salicylate, and <span class="name">E. coli</span> uses  
 +
3OC6-HSL, 3OC12-HSL and AI-2. In each case,  
 +
these signaling molecules correspond to rock, paper and scissors respectively.
</p>
</p>
 +
<p>
<p>
-
The next step is to design a way to know who wins the RPS game. That is when the E. coli judge team comes in! Each judge has an AND-gate promoter which produces a single output based on two inputs, human side and E.coli side signaling molecules. Since we want the output to be visible, we use either GFP, RFP or CFP to indicate whether humans win, lose or it is a tie, respectively.  
+
The next step is to design a way to know who wins the RPS game.  
 +
That is when the<span class="name"> E. coli</span> judge team comes in!  
 +
Each judge has an AND-gate promoter which produces a single output  
 +
based on two inputs, human side and<span class="name"> E.coli</span>
 +
side signaling molecules. Since we want the output to be visible,  
 +
we use either GFP, RFP or CFP to indicate whether humans win,  
 +
lose or it is a tie, respectively.  
</p>
</p>
 +
<p>
<p>
-
Lastly, we need to make sure E.coli follows the rules of the game by synthetizing only one signaling molecule every time it plays. Importantly, we have to design the opponent E.coli to be able to choose its signal randomly for a fare game, because E.coli will continue to lose if E.coli always produce same signaling molecule. In a view of these needs, we designed three randomizers that satisfy the conditions for the game. Single Colony Isolation, Survival of single strain and Conditional Knockout.
+
Lastly, we need to make sure <span  class="name">E. coli</span> follows  
-
<img src="https://static.igem.org/mediawiki/2011/1/10/TokyoTech_home_fig2.png" alt="RPS" style="float:none; text-align:center;"/>
+
the rules of the game by synthetizing only one signaling molecule  
 +
every time it plays. Importantly, we have to design the opponent  
 +
<span class="name">E. coli</span> to be able to choose its signal  
 +
randomly for a fair game, because <span class="name">E. coli</span>
 +
will continue to lose if it always produces the same signaling
 +
molecule. In a view of these needs, we designed three randomizers that
 +
satisfy the conditions for the game:Single Colony Isolation, Survival  
 +
of One Strain and Conditional Knockout.  
 +
<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/index.htm">(see more...)</a>
</p>
</p>
   
   
-
<h2 id="rain">3. Make it rain</h2>
+
<h2 id="rain"> Make it Rain </h2>
 +
<img src="https://static.igem.org/mediawiki/2011/7/7b/TokyoTech_home_fig3.png" alt="Raining" width="480px" class="fig" />
<p>
<p>
-
Playing RPS with E. coli during summer was fun, but, even if humans won, celebrations did not last long since we soon returned to complaining about the hot weather. As a prize for humans who win in our RPS game, we designed an E.coli that can make it rain, making the hot summer more fun and refreshing (let alone applications in agriculture). To make it rain we aimed to construct an isoprene synthetizing E.coli. Photo-oxidized isoprene acts as a condensation nucleus (Colin D. O‘Dowd et al., 2002, Nature) and can cause shower (make it rain) if only it is present in very low concentrations.<br />
+
Playing RPS with <span class="name">E. coli</span> during summer was fun, but,  
-
<img src="https://static.igem.org/mediawiki/2011/7/7b/TokyoTech_home_fig3.png" alt="Raining" style="float:none;"/>
+
even if humans won, joy did not last long since we soon returned to  
 +
complaining about the hot weather. As a prize for humans who win in our RPS game,  
 +
we designed an <span class="name">E. coli</span> that can make it rain,  
 +
making the hot summer more fun and refreshing (let alone applications in agriculture).  
 +
To make it rain, we constructed an isoprene synthetizing <span class="name">E. coli</span>.  
 +
Photo-oxidized isoprene acts as a condensation nucleus(Leonardo Silva Santos <i>et al</i>.,2006),
 +
which might cause rain, even in very low concentrations.
 +
<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm">(see more...)</a>
</p>
</p>
   
   
-
<h2 id="urea">4. Urea cooler</h2>
+
<h2 id="urea"> Urea Coolers </h2>
 +
<img src="https://static.igem.org/mediawiki/2011/e/ef/Home-urea.png" alt="Urea cooler" width="480px" class="fig" />
<p>
<p>
-
Coolers can be made by adding urea to water, since dissolving urea in water is an endothermic reaction (-57.8 cal/g). By only making arginase production in E. coli, we can make the bacterium synthetizeing urea!  Furthermore, we increased urea production by introduction of Arg Box sequence, which absorb ArgR repressor, at a site distant from promoters.<br />
+
Coolers can be made by adding urea to water, since dissolving urea in water  
-
To analyze how we obtain even more urea from our E.coli, elementary flux analysis (Schuster 2000) identified metabolic routes that are both stoichiometrically and thermodynamically feasible for a group of enzymes. Making use of it we can tell which substrates and pathways are the best for obtaining more urea.<br />  
+
is an endothermic reaction (-57.8 cal/g). We can make <span class="name">E.coli</span>  
-
<img src="https://static.igem.org/mediawiki/2011/3/39/TokyoTech_home_fig4.png" alt="Urea cooler" style="float:none"/>
+
synthetize urea just by introducing a gene encoding arginase!
</p>
</p>
-
 
+
<p>
 +
To analyze how we obtain even more urea from our <span class="name">E. coli</span>,
 +
we used elementary flux analysis (Schuste <i>et al.</i>, 2000) to identify
   
   
-
+
metabolic routes that are both stoichiometrically and thermodynamically
 +
 
 +
feasible for a group of enzymes. Making use of it we can tell which
 +
 +
                substrates and pathways are the best for obtaining
 +
               
 +
                more urea.<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm">(see more...)</a>
 +
</p>
 +
 
 +
<h2 id="manabi"> Human Practice </h2>
 +
<img src="https://static.igem.org/mediawiki/2011/2/25/Creating_Perception_1.png" alt="Creating Perception" width="480px" class="fig" />
 +
<p>
 +
We love Synthetic Biology, and to share our passion with other people,
 +
we did educational activities as part of our human practices.
 +
We would like to spread the word about what is made and aimed by synthetic 
 +
biology and iGEM. To do so, we created the &ldquo;iGEM Card Game&rdquo; and several 
 +
                posters. We also measured our impact through a survey.
 +
<a href="https://2011.igem.org/Team:Tokyo_Tech/HumanPractice.htm">(see more...)</a>
 +
</p>
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Latest revision as of 16:44, 28 October 2011


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iGEM Tokyo Tech 2011 Team

Overview: Cool down in summer with our Rock-Paper-Scissors Game

Project Overview

When summer comes it brings vacations, so students have plenty time to have fun. But summer means hot weather! In light of these circumstances, we designed a game that can be played between E.coli and humans. The winner of the game can get refreshing prizes! Dear students and judges, let us introduce you to the first human-bacteria Rock-Paper-Scissors game! Win and get a rain shower and a “urea cooler”!

Rock-Paper-Scissors (RPS) Game

RPS

So, how to play RPS with a handless bacteria? Instead of hands, we use different signaling molecules corresponding either to rock, paper or scissors. In our set of six signaling molecules, humans use IPTG, aTc and salicylate, and E. coli uses 3OC6-HSL, 3OC12-HSL and AI-2. In each case, these signaling molecules correspond to rock, paper and scissors respectively.

The next step is to design a way to know who wins the RPS game. That is when the E. coli judge team comes in! Each judge has an AND-gate promoter which produces a single output based on two inputs, human side and E.coli side signaling molecules. Since we want the output to be visible, we use either GFP, RFP or CFP to indicate whether humans win, lose or it is a tie, respectively.

Lastly, we need to make sure E. coli follows the rules of the game by synthetizing only one signaling molecule every time it plays. Importantly, we have to design the opponent E. coli to be able to choose its signal randomly for a fair game, because E. coli will continue to lose if it always produces the same signaling molecule. In a view of these needs, we designed three randomizers that satisfy the conditions for the game:Single Colony Isolation, Survival of One Strain and Conditional Knockout. (see more...)

Make it Rain

Raining

Playing RPS with E. coli during summer was fun, but, even if humans won, joy did not last long since we soon returned to complaining about the hot weather. As a prize for humans who win in our RPS game, we designed an E. coli that can make it rain, making the hot summer more fun and refreshing (let alone applications in agriculture). To make it rain, we constructed an isoprene synthetizing E. coli. Photo-oxidized isoprene acts as a condensation nucleus(Leonardo Silva Santos et al.,2006), which might cause rain, even in very low concentrations. (see more...)

Urea Coolers

Urea cooler

Coolers can be made by adding urea to water, since dissolving urea in water is an endothermic reaction (-57.8 cal/g). We can make E.coli synthetize urea just by introducing a gene encoding arginase!

To analyze how we obtain even more urea from our E. coli, we used elementary flux analysis (Schuste et al., 2000) to identify metabolic routes that are both stoichiometrically and thermodynamically feasible for a group of enzymes. Making use of it we can tell which substrates and pathways are the best for obtaining more urea.(see more...)

Human Practice

Creating Perception

We love Synthetic Biology, and to share our passion with other people, we did educational activities as part of our human practices. We would like to spread the word about what is made and aimed by synthetic biology and iGEM. To do so, we created the “iGEM Card Game” and several posters. We also measured our impact through a survey. (see more...)

Return to Page Top