Team:Tokyo Tech
From 2011.igem.org
Line 2: | Line 2: | ||
<html lang="english" xmlns="http://www.w3.org/1999/xhtml" xml:lang="english"> | <html lang="english" xmlns="http://www.w3.org/1999/xhtml" xml:lang="english"> | ||
<head> | <head> | ||
+ | <title>Tokyo Tech 2011</title> | ||
<meta http-equiv="content-script-type" content="text/javascript" /> | <meta http-equiv="content-script-type" content="text/javascript" /> | ||
<meta http-equiv="content-type" content="text/html; charset=Shift_JIS" /> | <meta http-equiv="content-type" content="text/html; charset=Shift_JIS" /> | ||
Line 76: | Line 77: | ||
background-color: #EFEBEC; | background-color: #EFEBEC; | ||
color: #000000; | color: #000000; | ||
- | |||
} | } | ||
Line 85: | Line 85: | ||
clear: both; | clear: both; | ||
height: 180px; | height: 180px; | ||
- | width: | + | width: 752px; |
} | } | ||
.main | .main | ||
{ | { | ||
position: absolute; | position: absolute; | ||
- | + | left: 160px; | |
- | + | ||
width: auto; | width: auto; | ||
} | } | ||
Line 98: | Line 97: | ||
{ | { | ||
position: static; | position: static; | ||
- | top: | + | top: 300px; |
margin: 5px 5px 5px 5px; | margin: 5px 5px 5px 5px; | ||
padding: 0 0 0 0; | padding: 0 0 0 0; | ||
Line 146: | Line 145: | ||
} | } | ||
- | + | img.center | |
{ | { | ||
- | float: | + | float: center; |
- | } | + | } |
- | + | ||
--> | --> | ||
Line 192: | Line 190: | ||
} | } | ||
- | + | window.onload = function() { | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
var menu = window.document.getElementById('TopMenu'); | var menu = window.document.getElementById('TopMenu'); | ||
if(menu==null) | if(menu==null) | ||
Line 237: | Line 223: | ||
<!-- top box --> | <!-- top box --> | ||
<div class="top"> | <div class="top"> | ||
- | < | + | <OBJECT classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,0,0" WIDTH="965" HEIGHT="150" id="Yourfilename" ALIGN=""> |
- | + | <PARAM NAME=movie VALUE="https://static.igem.org/mediawiki/2011/4/4d/Header.swf"> <PARAM NAME=quality VALUE=high> <PARAM NAME=bgcolor VALUE=#FFFFFF> <EMBED src="https://static.igem.org/mediawiki/2011/4/4d/Header.swf" quality=high bgcolor=#FFFFFF WIDTH="965" HEIGHT="150" NAME="Yourfilename" ALIGN="" TYPE="application/x-shockwave-flash" PLUGINSPAGE="http://www.macromedia.com/go/getflashplayer"></EMBED> </OBJECT> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
<!-- list of top menu --> | <!-- list of top menu --> | ||
<div id="navigation"> | <div id="navigation"> | ||
Line 274: | Line 255: | ||
<li id="menu_Extra"> | <li id="menu_Extra"> | ||
More | More | ||
- | <ul | + | <ul> |
<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Safety">Safety</a></li> | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Safety">Safety</a></li> | ||
<li><a href="https://2011.igem.org/Team:Tokyo_Tech/Attribution_and_Contributions.htm">Attribution and Contributions</a></li> | <li><a href="https://2011.igem.org/Team:Tokyo_Tech/Attribution_and_Contributions.htm">Attribution and Contributions</a></li> | ||
Line 293: | Line 274: | ||
<!-- left menu list --> | <!-- left menu list --> | ||
- | <div style="min-height: | + | <div style="min-height:2200px; float: left"> |
- | <div id="LeftMenu" style="top: | + | <div id="LeftMenu" style="top:50px;"> |
<!--list of page menu: DO NOT WRITE LINKS NOT WRITTEN IN THIS PAGE --> | <!--list of page menu: DO NOT WRITE LINKS NOT WRITTEN IN THIS PAGE --> | ||
<ul> | <ul> | ||
Line 301: | Line 282: | ||
<li><a href="#rain"> Make it rain</a></li> | <li><a href="#rain"> Make it rain</a></li> | ||
<li><a href="#urea"> Urea Coolers</a></li> | <li><a href="#urea"> Urea Coolers</a></li> | ||
- | + | <li><a href="#manabi"> Human Practice</a></li> | |
</ul> | </ul> | ||
</div> | </div> | ||
Line 313: | Line 294: | ||
<h2 id="Overall"> Overview: Cool down in summer with our Rock-Paper-Scissors Game</h2> | <h2 id="Overall"> Overview: Cool down in summer with our Rock-Paper-Scissors Game</h2> | ||
- | <img src="https://static.igem.org/mediawiki/2011/2/24/Happy-rps.png" width=" | + | <img src="https://static.igem.org/mediawiki/2011/2/24/Happy-rps.png" width="50%" style="float:right;"/> |
<p> | <p> | ||
- | When summer comes it brings vacations, so students have plenty time to have fun. | + | When summer comes it brings vacations, so students have plenty time to have fun. But summer means hot weather! In light of these circumstances, we designed a game that can be played between<i> E.coli</i> and humans, and that the winner of the game can get refreshing prizes! Dear students, let us introduce you to the first human-bacteria Rock-Paper-Scissors game! Win and get a rain shower, plus a “urea cooler”! |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</p> | </p> | ||
- | <h2 id="RPS"> Rock-Paper-Scissors (RPS) Game </h2> | + | <h2 id="RPS"> Rock-Paper-Scissors (RPS) Game</h2> |
- | <img src="https://static.igem.org/mediawiki/2011/1/10/TokyoTech_home_fig2.png" alt="RPS | + | <img src="https://static.igem.org/mediawiki/2011/1/10/TokyoTech_home_fig2.png" alt="RPS" class="fig" /> |
<p> | <p> | ||
- | So, how to play RPS with a handless bacteria? | + | So, how to play RPS with a handless bacteria? Instead of hands, we use different signaling molecules corresponding either to rock, paper or scissors. In our set of six signaling molecules, humans use IPTG, aTc and salicylate, and E. coli uses 3O-C6-HSL, 3O-C12-HSL and AI-2. In each case, these signaling molecules correspond to rock, paper and scissors respectively. |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</p> | </p> | ||
- | |||
<p> | <p> | ||
- | The next step is to design a way to know who wins the RPS game. | + | The next step is to design a way to know who wins the RPS game. That is when the<i> E. coli</i> judge team comes in! Each judge has an AND-gate promoter which produces a single output based on two inputs, human side and<i> E.coli</i> side signaling molecules. Since we want the output to be visible, we use either GFP, RFP or CFP to indicate whether humans win, lose or it is a tie, respectively. |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</p> | </p> | ||
- | |||
<p> | <p> | ||
- | + | Lastly, we need to make sure <span class="name">E. coli</span> follows the rules of the game by synthetizing only one signaling molecule every time it plays. Importantly, we have to design the opponent <span class="name">E. coli</span> to be able to choose its signal randomly for a fare game, because <i>E. coli</i> will continue to lose if<i> E. coli</i> always produce same signaling molecule. In a view of these needs, we designed three randomizers that satisfy the conditions for the game. Single Colony Isolation, Survival of single strain and Conditional Knockout.<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/index.htm">(see more...)</a> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</p> | </p> | ||
- | <h2 id="rain"> Make it Rain </h2> | + | <h2 id="rain"> Make it Rain</h2> |
- | <img src="https://static.igem.org/mediawiki/2011/7/7b/TokyoTech_home_fig3.png" alt="Raining | + | <img src="https://static.igem.org/mediawiki/2011/7/7b/TokyoTech_home_fig3.png" alt="Raining" class="fig" /> |
<p> | <p> | ||
- | Playing RPS with < | + | Playing RPS with <i>E. coli</i> during summer was fun, but, even if humans won, pleasures did not last long since we soon returned to complaining about the hot weather. As a prize for humans who win in our RPS game, we designed an <i>E. coli</i> that can make it rain, making the hot summer more fun and refreshing (let alone applications in agriculture). To make it rain, we constructed an isoprene synthetizing <span class="name">E. coli</span>. Photo-oxidized isoprene acts as a condensation nucleus(Colin D. O‘Dowd <i> et al</i>., 2002, Nature), which might cause rain, even though it is present in very low concentrations.<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm">(see more...)</a><br /> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</p> | </p> | ||
- | <h2 id="urea"> Urea Coolers </h2> | + | <h2 id="urea">Urea Coolers</h2> |
- | <img src="https://static.igem.org/mediawiki/2011/e/ef/Home-urea.png" alt="Urea cooler | + | <img src="https://static.igem.org/mediawiki/2011/e/ef/Home-urea.png" alt="Urea cooler" class="fig" /> |
<p> | <p> | ||
- | + | Coolers can be made by adding urea to water, since dissolving urea in water is an endothermic reaction (-57.8 cal/g). We can make <i>E.coli</i> synthetize urea just by introducing a gene encoding arginase! | |
- | + | </p> | |
- | + | <p> | |
- | + | To analyze how we obtain even more urea from our <i>E. coli</i>, elementary flux analysis (Schuste<i>et al.</i> 2000) identified metabolic routes that are both stoichiometrically and thermodynamically feasible for a group of enzymes. Making use of it we can tell which substrates and pathways are the best for obtaining more urea.<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm">(see more...)</a> <br /> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</p> | </p> | ||
- | + | <h2 id="manabi"> Human Practice</h2> | |
- | + | <img src="https://static.igem.org/mediawiki/2011/2/22/Manabi-ppt2.png" alt="Creating Perception" class="fig" /> | |
- | + | <p> | |
- | + | We love Synthetic Biology, and to share our passion with other people, we did educational activities as part of our human practices. We would like to spread what is made and aimed by synthetic biology and iGEM. For this event, we created “iGEM Card Game”, posters and questionnaire.<a href="https://2011.igem.org/Team:Tokyo_Tech/HumanPractice.htm">(see more...)</a><br /> | |
- | + | </p> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
<!-- ############ End of main contents ############ --> | <!-- ############ End of main contents ############ --> | ||
Line 403: | Line 340: | ||
</p> | </p> | ||
<!-- end of under contents --> | <!-- end of under contents --> | ||
- | |||
- | |||
- | |||
- | |||
<!-- DO NOT WRITE UNDER HERE --> | <!-- DO NOT WRITE UNDER HERE --> | ||
</body> | </body> | ||
</html> | </html> |
Revision as of 15:12, 5 October 2011
iGEM Tokyo Tech 2011 Team
Overview: Cool down in summer with our Rock-Paper-Scissors Game
When summer comes it brings vacations, so students have plenty time to have fun. But summer means hot weather! In light of these circumstances, we designed a game that can be played between E.coli and humans, and that the winner of the game can get refreshing prizes! Dear students, let us introduce you to the first human-bacteria Rock-Paper-Scissors game! Win and get a rain shower, plus a “urea cooler”!
Rock-Paper-Scissors (RPS) Game
So, how to play RPS with a handless bacteria? Instead of hands, we use different signaling molecules corresponding either to rock, paper or scissors. In our set of six signaling molecules, humans use IPTG, aTc and salicylate, and E. coli uses 3O-C6-HSL, 3O-C12-HSL and AI-2. In each case, these signaling molecules correspond to rock, paper and scissors respectively.
The next step is to design a way to know who wins the RPS game. That is when the E. coli judge team comes in! Each judge has an AND-gate promoter which produces a single output based on two inputs, human side and E.coli side signaling molecules. Since we want the output to be visible, we use either GFP, RFP or CFP to indicate whether humans win, lose or it is a tie, respectively.
Lastly, we need to make sure E. coli follows the rules of the game by synthetizing only one signaling molecule every time it plays. Importantly, we have to design the opponent E. coli to be able to choose its signal randomly for a fare game, because E. coli will continue to lose if E. coli always produce same signaling molecule. In a view of these needs, we designed three randomizers that satisfy the conditions for the game. Single Colony Isolation, Survival of single strain and Conditional Knockout.(see more...)
Make it Rain
Playing RPS with E. coli during summer was fun, but, even if humans won, pleasures did not last long since we soon returned to complaining about the hot weather. As a prize for humans who win in our RPS game, we designed an E. coli that can make it rain, making the hot summer more fun and refreshing (let alone applications in agriculture). To make it rain, we constructed an isoprene synthetizing E. coli. Photo-oxidized isoprene acts as a condensation nucleus(Colin D. O‘Dowd et al., 2002, Nature), which might cause rain, even though it is present in very low concentrations.(see more...)
Urea Coolers
Coolers can be made by adding urea to water, since dissolving urea in water is an endothermic reaction (-57.8 cal/g). We can make E.coli synthetize urea just by introducing a gene encoding arginase!
To analyze how we obtain even more urea from our E. coli, elementary flux analysis (Schusteet al. 2000) identified metabolic routes that are both stoichiometrically and thermodynamically feasible for a group of enzymes. Making use of it we can tell which substrates and pathways are the best for obtaining more urea.(see more...)
Human Practice
We love Synthetic Biology, and to share our passion with other people, we did educational activities as part of our human practices. We would like to spread what is made and aimed by synthetic biology and iGEM. For this event, we created “iGEM Card Game”, posters and questionnaire.(see more...)