- visualization-

@plasmid extraction and DNA refine OriT, GFP, RFP

@DNA densitometry

@restriction emzyme(XbaⅠ, PstⅠ)

@electrophoresis Succeeded GFP, but other two samples were failure.

@restriction emzyme(XbaⅠ, PstⅠ) again

-Targeting-

-Make agur plates

Acorrding to protocol