Team:Tokyo-NoKoGen

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<td height=113 bgcolor="#0082ef"><p class="style36"><strong>Tokyo-NokoGen 2011</strong></p>
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<p class="style34 f-lp">Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology</p>
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<td height=126 bgcolor="#ffffff"><p class="style9 f-lp">Tokyo-NokoGen 2011</p>
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<td height=267 colspan=2 rowspan=6><img src="Resources/ecolion.jpeg" border=0 width=302 height=267 alt="EcoLion" style="float:left"></td>
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<td height=357 rowspan=7 bgcolor="#cdcdcd"><p class="style3">&nbsp;</p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen"><span class="style18" style="text-decoration:underline">Home</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Team"><span class="style18" style="text-decoration:underline">Team</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/memebers"><span class="style18" style="text-decoration:underline">Members</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Project"><span class="style18" style="text-decoration:underline">Project: EcoLion</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Parts"><span class="style18" style="text-decoration:underline">BioBricks</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/notebook"><span class="style18" style="text-decoration:underline">Notebook</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="Protocols"><span class="style18" style="text-decoration:underline">Protocols</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="attribution"><span class="style18" style="text-decoration:underline">Attribution</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/safety"><span class="style18" style="text-decoration:underline">Safety</span></a></span></strong></p>
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<p class="style3 f-lp"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/sponsors"><span class="style18" style="text-decoration:underline">Sponsors</span></a></span></strong></p>
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<td height=316><p><span class="style7">&lt;Project Description&gt;</span></p>
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<p><span class="style25">11</span><span class="style26">th</span><span class="style25"> of March 2011 &#8211; a date we will never forget &#8211; a devastating earthquake and tsunami hit the Tohoku area of Japan. This earthquake left a huge damage on us. Although it has been 4 months since the tragedy, people in Fukushima are still suffering today from the radioactive compound such as Cesium leaking from the corrupted nuclear power plant from the unforgettable earthquake.</span></p>
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<p><span class="style25">What can we do to help? &#8211; was the question we asked ourselves to come up with a plan to make an E. coli radioactive cleaner. It&nbsp;is an E. coli that can take in radioactive Cesium contained inside water. By gathering and collecting the&nbsp;E. coli radioactive cleaner&nbsp;after the Cesium has been taken up, we will be left with clean water without radioactive compounds.</span></p>
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<p><span class="style25">In Fukushima today, there is still tons of cesium contaminated water inside the nuclear power plant that has to be taken care of. Much of it has also leaked out into the city, the country, and into the ocean that has been detected in countries outside Japan. Many people fear that&nbsp;these&nbsp;Cesium might&nbsp;do us&nbsp;harm from its radioactivity.&nbsp;This is when our&nbsp;E. coli radioactive cleaner&nbsp;come into action. The&nbsp;E. coli radioactive cleaner&nbsp;that we made will decrease Cesium inside the contaminated water and so the water will become cleaner. The cleaned water can then be released into the ocean solving people&#8217;s worries to how to get rid of contaminated water.</span></p>
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<td height=40 colspan=4><p class="style12 f-lp"><em><span class="style13"><strong>E<span class="style2">. </span></strong></span><span class="style17">col</span><span class="style9">i </span></em><span class="style13"><strong>col</strong><span class="style2">lecting heavy metal </span><strong>ion</strong><span class="style2">s</span></span></p>
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<td height=209 colspan=5 rowspan=2><p class="style32"><strong>Project abstract</strong></p>
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<p class="style31 f-lp"><span class="style33"><span class="style15"> We developed the EcoLion, which collects heavy metals from the environment, aimed at cleaning up heavy-metal pollution as well as for the mining of valuable metals for industrial applications. A bacterial micro-compartment (BMC) was engineered into our </span><em><span class="style15">E. coli</span></em><span class="style15"> micromachine to act as a tank to accumulate heavy metals. Heavy metal ions, such as Zn2+, Cd2+, and As2+</span></span><span class="style29">, that are taken up by the cell will be trapped inside the tank by a metallothionein fused to a BMC-localizing tag. Using BMC in such a way will be very advantageous as it may achieve the accumulation of high concentrations of heavy metals in one place. The EcoLion that has stored heavy metals will be conveniently collected by light using phototaxis or self-aggregation. This EcoLion may ultimately be applied for collecting a number of different toxic or valuable molecules by using specific target-binding peptides or proteins.</span></p>
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Revision as of 11:06, 24 September 2011

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Tokyo-NokoGen 2011

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
EcoLion

 

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Members

Project: EcoLion

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Attribution

Safety

Sponsors
EcoLionTitle

E. coli collecting heavy metal ions

Project abstract

We developed the EcoLion, which collects heavy metals from the environment, aimed at cleaning up heavy-metal pollution as well as for the mining of valuable metals for industrial applications. A bacterial micro-compartment (BMC) was engineered into our E. coli micromachine to act as a tank to accumulate heavy metals. Heavy metal ions, such as Zn2+, Cd2+, and As2+, that are taken up by the cell will be trapped inside the tank by a metallothionein fused to a BMC-localizing tag. Using BMC in such a way will be very advantageous as it may achieve the accumulation of high concentrations of heavy metals in one place. The EcoLion that has stored heavy metals will be conveniently collected by light using phototaxis or self-aggregation. This EcoLion may ultimately be applied for collecting a number of different toxic or valuable molecules by using specific target-binding peptides or proteins.

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