Team:Tokyo-NoKoGen

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<p align=center><font size=7><B> We are</font> <font size=50 color=yellowgreen>NOKOGEN</font></P>
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<div><b><font size="4"><font class="Apple-style-span" color="#000000">&lt;Project Description&gt;</font></font></b></div>
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<p style="margin:0mm 0mm 0pt"><span lang="EN-US"><font face="Century"><font size="3"><font class="Apple-style-span" color="#000000">11</font></font><font size="2"><sup><font class="Apple-style-span" color="#000000">th</font></sup></font><font size="3"><font class="Apple-style-span" color="#000000"> of March 2011 – a date we will never forget – a devastating earthquake and tsunami hit the Tohoku area of Japan. This earthquake left a huge damage on us. Although it has been 4 months since the tragedy, people in Fukushima are still suffering today from the radioactive compound such as Cesium leaking from the corrupted nuclear power plant from the unforgettable earthquake.</font></font></font></span></p>
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<p style="margin:0mm 0mm 0pt"><font size="3"><span lang="EN-US"><font face="Century"><font class="Apple-style-span" color="#000000">What can we do to help? – was the question we asked ourselves to come up with a plan to make an </font><i><font class="Apple-style-span" color="#000000">E. coli</font></i><font class="Apple-style-span" color="#000000"> radioactive cleaner</font></font></span><span lang="EN-US"><font face="Century"><font class="Apple-style-span" color="#000000">. It</font></font></span><span lang="EN-US"><font face="Century"><font class="Apple-style-span" color="#000000">&nbsp;is an </font><i><font class="Apple-style-span" color="#000000">E. coli</font></i><font class="Apple-style-span" color="#000000"> that can take in radioactive Cesium contained inside water. By gathering and collecting the&nbsp;</font></font></span><span lang="EN-US"><font face="Century"><i><font class="Apple-style-span" color="#000000">E. coli</font></i><font class="Apple-style-span" color="#000000"> radioactive cleaner&nbsp;after the Cesium has been taken up, we will be left with clean water without radioactive compounds.</font></font></span></font></p>
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<p style="margin:0mm 0mm 0pt"><font size="3"><span lang="EN-US"><font face="Century"><font class="Apple-style-span" color="#000000">In Fukushima today, there is still tons of cesium contaminated water inside the nuclear power plant that has to be taken care of. Much of it has also leaked out into the city, the country, and into the ocean that has been detected in countries outside Japan. Many people fear that&nbsp;these&nbsp;Cesium might&nbsp;do us&nbsp;harm from its radioactivity.&nbsp;This is when our&nbsp;</font></font></span></font><span style="font-family: Century; line-height: 25px; font-size: medium; "><i><font class="Apple-style-span" color="#000000">E. coli </font></i><font class="Apple-style-span" color="#000000">radioactive cleaner</font></span><span style="line-height: 25px; font-size: medium; "><font face="Century"><font class="Apple-style-span" color="#000000">&nbsp;</font><span lang="EN-US"><font class="Apple-style-span" color="#000000">come into action. The&nbsp;</font></span></font><span lang="EN-US"><font face="Century"><i><font class="Apple-style-span" color="#000000">E. coli</font></i><font class="Apple-style-span" color="#000000"> radioactive cleaner&nbsp;that we made will decrease Cesium inside the contaminated water and so the water will become cleaner. The cleaned water can then be released into the ocean solving people’s worries to how to get rid of contaminated water.</font></font></span></span></p>
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<p style="margin:0mm 0mm 0pt"><span lang="EN-US"><font size="3"><font face="Century"><font class="Apple-style-span" color="#000000">We hope that people in Fukushima will be released from fear and stress and will be able to get back to their daily lives as soon as possible. </font><span><font class="Apple-style-span" color="#000000">&nbsp;</font></span></font></font></span></p>
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<div><span style="font-size:large">&lt;Safety Proposal&gt;</span></div>
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<td bgcolor="#ffff99" valign="top"><b><span style="font-size:medium">-Would any of your project ideas raise safety issues in terms of:&nbsp;</span></b><span style="font-size:medium"><br>
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<p><span><span style="font-size:medium">&nbsp;&nbsp;</span></span><span style="font-size:medium">Our project will not raise any issues in researcher safety, public safety and environmental safety. Our project will be carried out with </span><i><span style="font-size:medium">E. coli</span></i><span style="font-size:medium"> laboratory strains and we do not use any infectious organisms. Our laboratory work will be carried out in the laboratories of Prof. Sode (advisor), Assoc. Prof. Tsugawa (instructor) and Prof. Ikebukuro (instructor). Those laboratories have a proper facility of the biosafety level 2 to handle </span><i><span style="font-size:medium">E. coli</span></i><span style="font-size:medium"> and perform genetic engineering experiments.</span></p>
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<p><span><span style="font-size:medium">&nbsp; </span></span><span style="font-size:medium">In our project, we are trying to construct the </span><i><span style="font-size:medium">E. coli</span></i><span style="font-size:medium"> that absorbs Cesium from radioactive-contaminated water. The ultimate aim of our project is to develop an </span><i><span style="font-size:medium">E. coli</span></i><span style="font-size:medium"> machine that can be practically used to clean up radioactive-contaminated environment. However, genetically engineered organisms cannot be easily released to environment. All team members well understand this point. We are just aiming as biotechnologists at showing potential of synthetic biology for those applications that are harmful for humans and cannot be easily achieved by non-biological ways.</span></p>
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<b><span style="font-size:medium">-Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,&nbsp;</span></b><span style="font-size:medium"><br>
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<p><span style="font-size:medium">&nbsp;&nbsp; No,they don’t.&nbsp;Our BioBrick parts do not posess any safety risk for environment and researches. We will make a new BioBrick part that uptakes cesium from environment. This part is natively expressed in a non-harmful strain of E. coli and we will clone it from that strain. We are also trying to make new BioBrick parts to regulate gene expressing network.&nbsp;We will use those genes that already have analyzed in peer-reviewed papers.&nbsp;Thus that new BioBrick part will not raise any safety issues.&nbsp;</span></p>
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<b><span style="font-size:medium">-Is there a local biosafety group, committee, or review board at your institution?</span></b><span style="font-size:medium"><br>
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<p><span style="font-size:medium">&nbsp;&nbsp; Yes, there is. There is the committee of Tokyo University of
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Agriculture and Technology, and we are following its safety and ethical guidelines.</span></p>
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<b><span style="font-size:medium">-Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</span></b><span style="font-size:medium"><br>
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<p><span lang="EN-US"><span style="font-size:medium">&nbsp;&nbsp; Heinrich's Law: for every 300 unsafe acts (no-injury accidents), there are 29 minor injuries and 1 major injury.&nbsp;</span></span><span style="font-size:medium">So, minimizing the number of no­injury accidents will reduce the probability of more severe accidents.&nbsp;We suggest that all teams share any safety issues and solution timely on iGEM web site or wikis. That should prevent the occurrence of more serious hazards.</span></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen"><span class="style18" style="text-decoration:underline">Home</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Team"><span class="style18" style="text-decoration:underline">Team</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/memebers"><span class="style18" style="text-decoration:underline">Members</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Project"><span class="style18" style="text-decoration:underline">Project: EcoLion</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/notebook"><span class="style18" style="text-decoration:underline">Notebook</span></a></span></strong></p>
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<p class="style3 f-lp"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/sponsors"><span class="style18" style="text-decoration:underline">Sponsors</span></a></span></strong></p>
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<td height=40 colspan=5><p class="style12 f-lp"><em><span class="style13"><strong>E<span class="style2">. </span></strong></span><span class="style17">col</span><span class="style9">i </span></em><span class="style13"><strong>col</strong><span class="style2">lecting heavy metal </span><strong>ion</strong><span class="style2">s</span></span></p>
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<td height=144 colspan=5 rowspan=2><p class="f-lp"><span class="style28">We developed the EcoLion, which collects heavy metals from the environment, aimed at cleaning up heavy-metal pollution as well as for the mining of valuable metals for industrial applications. A bacterial micro-compartment (BMC) was engineered into our <em>E. coli</em> micromachine to act as a tank to accumulate heavy metals. Heavy metal ions, such as Zn2+, Cd2+, and As2+, that are taken up by the cell will be trapped inside the tank by a metallothionein fused to a BMC-localizing tag. Using BMC in such a way will be very advantageous as it may achieve the accumulation of high concentrations of heavy metals in one place. The EcoLion that has stored heavy metals will be conveniently collected by light using phototaxis or self-aggregation. This EcoLion may ultimately be applied for collecting a number of different toxic or valuable molecules by using specific target-binding peptides or proteins.</span></p>
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!align="center"|[[Team:Tokyo-NoKoGen|Home]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Tokyo-NoKoGen Official Team Profile]
 
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Latest revision as of 01:27, 6 October 2011

Home

Tokyo-NokoGen 2011

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
EcoLion

 

Home

Team

Members

Project: EcoLion

BioBricks

Notebook

Protocols

Attribution

Safety

Sponsors
EcoLionTitle

E. coli collecting heavy metal ions

Project abstract

We developed the EcoLion, which collects heavy metals from the environment, aimed at cleaning up heavy-metal pollution as well as for the mining of valuable metals for industrial applications. A bacterial micro-compartment (BMC) was engineered into our E. coli micromachine to act as a tank to accumulate heavy metals. Heavy metal ions, such as Zn2+, Cd2+, and As2+, that are taken up by the cell will be trapped inside the tank by a metallothionein fused to a BMC-localizing tag. Using BMC in such a way will be very advantageous as it may achieve the accumulation of high concentrations of heavy metals in one place. The EcoLion that has stored heavy metals will be conveniently collected by light using phototaxis or self-aggregation. This EcoLion may ultimately be applied for collecting a number of different toxic or valuable molecules by using specific target-binding peptides or proteins.

Retrieved from "http://2011.igem.org/Team:Tokyo-NoKoGen"