Team:Tokyo-NoKoGen

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<td height=267 colspan=2 rowspan=6><img src="http://2011.igem.org/wiki/images/6/6a/EcoLion.png" border=0 width=302 height=267 alt="EcoLion" style="float:left"></td>
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<td height=357 rowspan=7 bgcolor="#cdcdcd"><p class="style3">&nbsp;</p>
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<td height=357 rowspan=9 bgcolor="#cdcdcd"><p class="style3">&nbsp;</p>
<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen"><span class="style18" style="text-decoration:underline">Home</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen"><span class="style18" style="text-decoration:underline">Home</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/Team"><span class="style18" style="text-decoration:underline">Team</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/Team"><span class="style18" style="text-decoration:underline">Team</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/Parts"><span class="style18" style="text-decoration:underline">BioBricks</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/Parts"><span class="style18" style="text-decoration:underline">BioBricks</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/notebook"><span class="style18" style="text-decoration:underline">Notebook</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/notebook"><span class="style18" style="text-decoration:underline">Notebook</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="Protocols"><span class="style18" style="text-decoration:underline">Protocols</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/protocols"><span class="style18" style="text-decoration:underline">Protocols</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="attribution"><span class="style18" style="text-decoration:underline">Attribution</span></a></span></strong></p>
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<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/attribution"><span class="style18" style="text-decoration:underline">Attribution</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/safety"><span class="style18" style="text-decoration:underline">Safety</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/safety"><span class="style18" style="text-decoration:underline">Safety</span></a></span></strong></p>
<p class="style3 f-lp"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/sponsors"><span class="style18" style="text-decoration:underline">Sponsors</span></a></span></strong></p>
<p class="style3 f-lp"><strong><span class="style14"><a href="http://2011.igem.org/Team:Tokyo-NoKoGen/sponsors"><span class="style18" style="text-decoration:underline">Sponsors</span></a></span></strong></p>
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<td height=104><img src="http://2011.igem.org/wiki/images/e/e4/EcoLion_Title.jpg" border=0 width=317 height=104 alt="EcoLionTitle" style="float:left"></td>
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<td height=40 colspan=4><p class="style12 f-lp"><em><span class="style13"><strong>E<span class="style2">. </span></strong></span><span class="style17">col</span><span class="style9">i </span></em><span class="style13"><strong>col</strong><span class="style2">lecting heavy metal </span><strong>ion</strong><span class="style2">s</span></span></p>
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<td height=209 colspan=5 rowspan=2><p class="style32"><strong>Project abstract</strong></p>
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<p class="style31 f-lp"><span class="style33"><span class="style15"> We developed the EcoLion, which collects heavy metals from the environment, aimed at cleaning up heavy-metal pollution as well as for the mining of valuable metals for industrial applications. A bacterial micro-compartment (BMC) was engineered into our </span><em><span class="style15">E. coli</span></em><span class="style15"> micromachine to act as a tank to accumulate heavy metals. Heavy metal ions, such as Zn2+, Cd2+, and As2+</span></span><span class="style29">, that are taken up by the cell will be trapped inside the tank by a metallothionein fused to a BMC-localizing tag. Using BMC in such a way will be very advantageous as it may achieve the accumulation of high concentrations of heavy metals in one place. The EcoLion that has stored heavy metals will be conveniently collected by light using phototaxis or self-aggregation. This EcoLion may ultimately be applied for collecting a number of different toxic or valuable molecules by using specific target-binding peptides or proteins.</span></p>
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<td height=144 colspan=5 rowspan=2><p class="f-lp"><span class="style28">We developed the EcoLion, which collects heavy metals from the environment, aimed at cleaning up heavy-metal pollution as well as for the mining of valuable metals for industrial applications. A bacterial micro-compartment (BMC) was engineered into our <em>E. coli</em> micromachine to act as a tank to accumulate heavy metals. Heavy metal ions, such as Zn2+, Cd2+, and As2+, that are taken up by the cell will be trapped inside the tank by a metallothionein fused to a BMC-localizing tag. Using BMC in such a way will be very advantageous as it may achieve the accumulation of high concentrations of heavy metals in one place. The EcoLion that has stored heavy metals will be conveniently collected by light using phototaxis or self-aggregation. This EcoLion may ultimately be applied for collecting a number of different toxic or valuable molecules by using specific target-binding peptides or proteins.</span></p>
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Latest revision as of 01:27, 6 October 2011

Home

Tokyo-NokoGen 2011

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

EcoLion

 

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Project: EcoLion

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Attribution

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EcoLionTitle

E. coli collecting heavy metal ions

Project abstract

We developed the EcoLion, which collects heavy metals from the environment, aimed at cleaning up heavy-metal pollution as well as for the mining of valuable metals for industrial applications. A bacterial micro-compartment (BMC) was engineered into our E. coli micromachine to act as a tank to accumulate heavy metals. Heavy metal ions, such as Zn2+, Cd2+, and As2+, that are taken up by the cell will be trapped inside the tank by a metallothionein fused to a BMC-localizing tag. Using BMC in such a way will be very advantageous as it may achieve the accumulation of high concentrations of heavy metals in one place. The EcoLion that has stored heavy metals will be conveniently collected by light using phototaxis or self-aggregation. This EcoLion may ultimately be applied for collecting a number of different toxic or valuable molecules by using specific target-binding peptides or proteins.