Team:Tianjin/Data
From 2011.igem.org
Line 357: | Line 357: | ||
<tr> | <tr> | ||
<td style="background:#ffdfbf;">1</td> | <td style="background:#ffdfbf;">1</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563000</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563000">BBa_K563000</a></td> |
<td style="background:#ffdfbf;">Regulatory</td> | <td style="background:#ffdfbf;">Regulatory</td> | ||
<td style="background:#ffdfbf;">Promotor of HIS4</td> | <td style="background:#ffdfbf;">Promotor of HIS4</td> | ||
Line 365: | Line 365: | ||
<tr> | <tr> | ||
<td>2</td> | <td>2</td> | ||
- | <td>BBa_K563001</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563001">BBa_K563001</a></td> |
<td>Regulatory</td> | <td>Regulatory</td> | ||
<td>Promoter of DAL7</td> | <td>Promoter of DAL7</td> | ||
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<tr> | <tr> | ||
<td style="background:#ffdfbf;">3</td> | <td style="background:#ffdfbf;">3</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563002</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563002">BBa_K563002</a></td> |
<td style="background:#ffdfbf;">Regulatory</td> | <td style="background:#ffdfbf;">Regulatory</td> | ||
<td style="background:#ffdfbf;">Promoter of ILV1</td> | <td style="background:#ffdfbf;">Promoter of ILV1</td> | ||
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<tr> | <tr> | ||
<td>4</td> | <td>4</td> | ||
- | <td>BBa_K563003</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563003">BBa_K563003</a></td> |
<td>Regulatory</td> | <td>Regulatory</td> | ||
<td>Promoter of CIT2</td> | <td>Promoter of CIT2</td> | ||
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<tr> | <tr> | ||
<td style="background:#ffdfbf;">5</td> | <td style="background:#ffdfbf;">5</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563004</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563004">BBa_K563004</a></td> |
<td style="background:#ffdfbf;">Regulatory</td> | <td style="background:#ffdfbf;">Regulatory</td> | ||
<td style="background:#ffdfbf;">Promoter of TEF1</td> | <td style="background:#ffdfbf;">Promoter of TEF1</td> | ||
Line 397: | Line 397: | ||
<tr> | <tr> | ||
<td>6</td> | <td>6</td> | ||
- | <td>BBa_K563010</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563010">BBa_K563010</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Original TOR</td> | <td>Original TOR</td> | ||
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<tr> | <tr> | ||
<td style="background:#ffdfbf;">7</td> | <td style="background:#ffdfbf;">7</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563011</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563011">BBa_K563011</a></td> |
<td style="background:#ffdfbf;">Coding</td> | <td style="background:#ffdfbf;">Coding</td> | ||
<td style="background:#ffdfbf;">TOR (LP Mutation)</td> | <td style="background:#ffdfbf;">TOR (LP Mutation)</td> | ||
Line 413: | Line 413: | ||
<tr> | <tr> | ||
<td>8</td> | <td>8</td> | ||
- | <td>BBa_K563012</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563012">BBa_K563012</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>TOR (ITMutation)</td> | <td>TOR (ITMutation)</td> | ||
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<tr> | <tr> | ||
<td style="background:#ffdfbf;">9</td> | <td style="background:#ffdfbf;">9</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563013</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563013">BBa_K563013</a></td> |
<td style="background:#ffdfbf;">Coding</td> | <td style="background:#ffdfbf;">Coding</td> | ||
<td style="background:#ffdfbf;">TOR (IT/EK Mutation)</td> | <td style="background:#ffdfbf;">TOR (IT/EK Mutation)</td> | ||
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<tr> | <tr> | ||
<td>10</td> | <td>10</td> | ||
- | <td>BBa_K563014</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563014">BBa_K563014</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>TOR (IT/VA Mutation)</td> | <td>TOR (IT/VA Mutation)</td> | ||
Line 437: | Line 437: | ||
<tr> | <tr> | ||
<td style="background:#ffdfbf;">11</td> | <td style="background:#ffdfbf;">11</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563015</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563015">BBa_K563015</a></td> |
<td style="background:#ffdfbf;">Coding</td> | <td style="background:#ffdfbf;">Coding</td> | ||
<td style="background:#ffdfbf;">TOR (EK/VA Mutation)</td> | <td style="background:#ffdfbf;">TOR (EK/VA Mutation)</td> | ||
Line 445: | Line 445: | ||
<tr> | <tr> | ||
<td>12</td> | <td>12</td> | ||
- | <td>BBa_K563016</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563016">BBa_K563016</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>TOR (IT/VA/EK Mutation)</td> | <td>TOR (IT/VA/EK Mutation)</td> | ||
Line 453: | Line 453: | ||
<tr> | <tr> | ||
<td style="background:#ffdfbf;">13</td> | <td style="background:#ffdfbf;">13</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563030</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563030">BBa_K563030</a></td> |
<td style="background:#ffdfbf;">Coding</td> | <td style="background:#ffdfbf;">Coding</td> | ||
<td style="background:#ffdfbf;">pEASY-TOR01</td> | <td style="background:#ffdfbf;">pEASY-TOR01</td> | ||
Line 461: | Line 461: | ||
<tr> | <tr> | ||
<td>14</td> | <td>14</td> | ||
- | <td>BBa_K563031</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563031">BBa_K563031</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>pEASY-TOR11</td> | <td>pEASY-TOR11</td> | ||
Line 469: | Line 469: | ||
<tr> | <tr> | ||
<td style="background:#ffdfbf;">15</td> | <td style="background:#ffdfbf;">15</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563032</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563032">BBa_K563032</a></td> |
<td style="background:#ffdfbf;">Coding</td> | <td style="background:#ffdfbf;">Coding</td> | ||
<td style="background:#ffdfbf;">pEASY-TOR22</td> | <td style="background:#ffdfbf;">pEASY-TOR22</td> | ||
Line 477: | Line 477: | ||
<tr> | <tr> | ||
<td>16</td> | <td>16</td> | ||
- | <td>BBa_K563033</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563033">BBa_K563033</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>pEASY-TOR33</td> | <td>pEASY-TOR33</td> | ||
Line 485: | Line 485: | ||
<tr> | <tr> | ||
<td style="background:#ffdfbf;">17</td> | <td style="background:#ffdfbf;">17</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563034</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563034">BBa_K563034</a></td> |
<td style="background:#ffdfbf;">Coding</td> | <td style="background:#ffdfbf;">Coding</td> | ||
<td style="background:#ffdfbf;">pEASY-TOR22(LP Mutation)</td> | <td style="background:#ffdfbf;">pEASY-TOR22(LP Mutation)</td> | ||
Line 493: | Line 493: | ||
<tr> | <tr> | ||
<td>18</td> | <td>18</td> | ||
- | <td>BBa_K563035</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563035">BBa_K563035</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>pEASY-TOR33(IT Mutation)</td> | <td>pEASY-TOR33(IT Mutation)</td> | ||
Line 501: | Line 501: | ||
<tr> | <tr> | ||
<td style="background:#ffdfbf;">19</td> | <td style="background:#ffdfbf;">19</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563036</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563036">BBa_K563036</a></td> |
<td style="background:#ffdfbf;">Coding</td> | <td style="background:#ffdfbf;">Coding</td> | ||
<td style="background:#ffdfbf;">pEASY-TOR33(VA Mutation)</td> | <td style="background:#ffdfbf;">pEASY-TOR33(VA Mutation)</td> | ||
Line 509: | Line 509: | ||
<tr> | <tr> | ||
<td>20</td> | <td>20</td> | ||
- | <td>BBa_K563037</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563037">BBa_K563037</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>pEASY-TOR33(EK Mutation)</td> | <td>pEASY-TOR33(EK Mutation)</td> | ||
Line 517: | Line 517: | ||
<tr> | <tr> | ||
<td style="background:#ffdfbf;">21</td> | <td style="background:#ffdfbf;">21</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563040</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563040">BBa_K563040</a></td> |
<td style="background:#ffdfbf;">Reporter</td> | <td style="background:#ffdfbf;">Reporter</td> | ||
<td style="background:#ffdfbf;">pYE-EGFP</td> | <td style="background:#ffdfbf;">pYE-EGFP</td> | ||
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<tr> | <tr> | ||
<td>22</td> | <td>22</td> | ||
- | <td>BBa_K563041</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563041">BBa_K563041</a></td> |
<td>Reporter</td> | <td>Reporter</td> | ||
<td>pYE-ERFP</td> | <td>pYE-ERFP</td> | ||
Line 531: | Line 531: | ||
<td>744</td> | <td>744</td> | ||
</tr> | </tr> | ||
- | <tr | + | <tr> |
<td style="background:#ffdfbf;">23</td> | <td style="background:#ffdfbf;">23</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563042</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563042">BBa_K563042</a></td> |
<td style="background:#ffdfbf;">Reporter</td> | <td style="background:#ffdfbf;">Reporter</td> | ||
<td style="background:#ffdfbf;">pYE-EOFP</td> | <td style="background:#ffdfbf;">pYE-EOFP</td> | ||
Line 541: | Line 541: | ||
<tr> | <tr> | ||
<td>24</td> | <td>24</td> | ||
- | <td>BBa_K563043</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563043">BBa_K563043</a></td> |
<td>Reporter</td> | <td>Reporter</td> | ||
<td>pYE-EYFP</td> | <td>pYE-EYFP</td> | ||
Line 549: | Line 549: | ||
<tr> | <tr> | ||
<td style="background:#ffdfbf;">25</td> | <td style="background:#ffdfbf;">25</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563044</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563044">BBa_K563044</a></td> |
<td style="background:#ffdfbf;">Reporter</td> | <td style="background:#ffdfbf;">Reporter</td> | ||
<td style="background:#ffdfbf;">pYE-EPIC Firefly Luciferase</td> | <td style="background:#ffdfbf;">pYE-EPIC Firefly Luciferase</td> | ||
Line 557: | Line 557: | ||
<tr> | <tr> | ||
<td>26</td> | <td>26</td> | ||
- | <td>BBa_K563045</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563045">BBa_K563045</a></td> |
<td>Reporter</td> | <td>Reporter</td> | ||
<td>pYE-OFL Orange Firefly Luciferase</td> | <td>pYE-OFL Orange Firefly Luciferase</td> | ||
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<tr> | <tr> | ||
<td style="background:#ffdfbf;">27</td> | <td style="background:#ffdfbf;">27</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563046</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563046">BBa_K563046</a></td> |
<td style="background:#ffdfbf;">Reporter</td> | <td style="background:#ffdfbf;">Reporter</td> | ||
<td style="background:#ffdfbf;">pYE-LacZ</td> | <td style="background:#ffdfbf;">pYE-LacZ</td> | ||
Line 573: | Line 573: | ||
<tr> | <tr> | ||
<td>28</td> | <td>28</td> | ||
- | <td>BBa_K563050</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563050">BBa_K563050</a></td> |
<td>Plasmid Backbone</td> | <td>Plasmid Backbone</td> | ||
<td>pSB1A11(Modified from pSB1A2)</td> | <td>pSB1A11(Modified from pSB1A2)</td> | ||
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<tr> | <tr> | ||
<td style="background:#ffdfbf;">29</td> | <td style="background:#ffdfbf;">29</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563051</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563051">BBa_K563051</a></td> |
<td style="background:#ffdfbf;">Coding</td> | <td style="background:#ffdfbf;">Coding</td> | ||
<td style="background:#ffdfbf;">TOR in pSBY1</td> | <td style="background:#ffdfbf;">TOR in pSBY1</td> | ||
Line 589: | Line 589: | ||
<tr> | <tr> | ||
<td>30</td> | <td>30</td> | ||
- | <td>BBa_K563052</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563052">BBa_K563052</a></td> |
<td>Plasmid</td> | <td>Plasmid</td> | ||
<td>pSBY11(pSB1A11-TRP1 CEN/ARS Yeast Shuttle Vector)</td> | <td>pSBY11(pSB1A11-TRP1 CEN/ARS Yeast Shuttle Vector)</td> | ||
Line 597: | Line 597: | ||
<tr> | <tr> | ||
<td style="background:#ffdfbf;">31</td> | <td style="background:#ffdfbf;">31</td> | ||
- | <td style="background:#ffdfbf;">BBa_K563053</td> | + | <td style="background:#ffdfbf;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563053">BBa_K563053</a></td> |
<td style="background:#ffdfbf;">Plasmid Backbone</td> | <td style="background:#ffdfbf;">Plasmid Backbone</td> | ||
<td style="background:#ffdfbf;">pYE</td> | <td style="background:#ffdfbf;">pYE</td> | ||
Line 605: | Line 605: | ||
<tr> | <tr> | ||
<td>32</td> | <td>32</td> | ||
- | <td>BBa_K563054</td> | + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K563054">BBa_K563054</a></td> |
<td>Plasmid Backbone</td> | <td>Plasmid Backbone</td> | ||
<td>pYD</td> | <td>pYD</td> |
Revision as of 15:29, 5 October 2011
Template:Https://2011.igem.org/Team:Peking S/bannerhidden Template:Https://2011.igem.org/Team:Peking S/back2
This page shows a list of all the parts that we have made or used in the project. Click on each link to see more details about that part on the Registry of Standard Biological Parts. As our project focus on tor2 gene coming from yeast and contains common restriction sites like EcoRI, NotI and SpeI, the stantard Plasmid Backbone and BioBrick prefix/suffix is no longer appropriate. Furthermore, the size of 7800bp makes it pretty too expensive and inconvenient for chemical synthesis and codon optimization to avoid certain restriction sites. To match with the core gene and protein, a series of parts also give up standard prefix/suffix and backbones. We have used at least 3 kinds of plasmids, including blunt-end vectors to Yeast Surface Display Vector. However, based on the splendid standard plasmid backbone pSB1C3, which has a small size and simple MCS for easy-operation, we modified several vectors, which also render functionality and ease-of-use of promoter parts.
1. Inducible pCIT2 – Mut-TOR2 - Terminator, BBa_K563061: Promoter of CIT2 gene would be binding with transcription factor RTG1/3 at the presence of FAP. Then our reconstructed Mut-TOR2 would be expressed and forms in Mut-TORC1 which is not allergic to FAP. The accumulation of Mut-TORC1 will help the intracellular phosphorylation back to normal state. 2. pYE- Reporter, BBa_K563040 ~ BBa_K563046: We have successfully construct vectors, which coding bright fluorescent protein and Luciferase and beta-galactosidase in yeast cell. This series of BioBricks provide a convenient method for the measurement and detection of gene expression and terminate the unsuccessful expression of fluorescent protein in yeast cell. 3. Modified Plasmid Backbone – pSBY11, BBa_K563051: We reconstruct the Yeast Vector pYD through the combination with pSB1A11. According to specific primers, we amplify the yeast elements (CEN4/ARS6, TRP1 orf and terminator region) by PCR, and then insert the fragment into pSB1A11 by single restriction site PstI. In this way, a modified vector that combines pYD and pSB1A11 together(pSBY1) for large fragment transformation between yeast and E. coli is built.Name | Type | Description | Designer | Length | |
---|---|---|---|---|---|
1 | BBa_K563000 | Regulatory | Promotor of HIS4 | Boxuan Zeng | 508 |
2 | BBa_K563001 | Regulatory | Promoter of DAL7 | Boxuan Zeng | 498 |
3 | BBa_K563002 | Regulatory | Promoter of ILV1 | Boxuan Zeng | 509 |
4 | BBa_K563003 | Regulatory | Promoter of CIT2 | Boxuan Zeng | 497 |
5 | BBa_K563004 | Regulatory | Promoter of TEF1 | Boxuan Zeng | 450 |
6 | BBa_K563010 | Coding | Original TOR | Boxuan Zeng | 8118 |
7 | BBa_K563011 | Coding | TOR (LP Mutation) | Boxuan Zeng | 8118 |
8 | BBa_K563012 | Coding | TOR (ITMutation) | Boxuan Zeng | 8118 |
9 | BBa_K563013 | Coding | TOR (IT/EK Mutation) | Boxuan Zeng | 8118 |
10 | BBa_K563014 | Coding | TOR (IT/VA Mutation) | Boxuan Zeng | 8118 |
11 | BBa_K563015 | Coding | TOR (EK/VA Mutation) | Boxuan Zeng | 8118 |
12 | BBa_K563016 | Coding | TOR (IT/VA/EK Mutation) | Boxuan Zeng | 8118 |
13 | BBa_K563030 | Coding | pEASY-TOR01 | Wenbin Kuang | 3067 |
14 | BBa_K563031 | Coding | pEASY-TOR11 | Wenbin Kuang | 2521 |
15 | BBa_K563032 | Coding | pEASY-TOR22 | Wenbin Kuang | 2709 |
16 | BBa_K563033 | Coding | pEASY-TOR33 | Wenbin Kuang | 2499 |
17 | BBa_K563034 | Coding | pEASY-TOR22(LP Mutation) | Mingrui Zhao | 2709 |
18 | BBa_K563035 | Coding | pEASY-TOR33(IT Mutation) | Mingrui Zhao | 2499 |
19 | BBa_K563036 | Coding | pEASY-TOR33(VA Mutation) | Jing Guo | 2499 |
20 | BBa_K563037 | Coding | pEASY-TOR33(EK Mutation) | Jing Guo | 2499 |
21 | BBa_K563040 | Reporter | pYE-EGFP | Bin Jia | 739 |
22 | BBa_K563041 | Reporter | pYE-ERFP | Bin Jia | 744 |
23 | BBa_K563042 | Reporter | pYE-EOFP | Bin Jia | 744 |
24 | BBa_K563043 | Reporter | pYE-EYFP | Bin Jia | 753 |
25 | BBa_K563044 | Reporter | pYE-EPIC Firefly Luciferase | Bin Jia | 1656 |
26 | BBa_K563045 | Reporter | pYE-OFL Orange Firefly Luciferase | Bin Jia | 1656 |
27 | BBa_K563046 | Reporter | pYE-LacZ | Bin Jia | 3000 |
28 | BBa_K563050 | Plasmid Backbone | pSB1A11(Modified from pSB1A2) | Bin Jia | 2090 |
29 | BBa_K563051 | Coding | TOR in pSBY1 | Bin Jia | 11914 |
30 | BBa_K563052 | Plasmid | pSBY11(pSB1A11-TRP1 CEN/ARS Yeast Shuttle Vector) | Bin Jia | 3830 |
31 | BBa_K563053 | Plasmid Backbone | pYE | Bin Jia | 5900 |
32 | BBa_K563054 | Plasmid Backbone | pYD | Bin Jia | 5090 |