Team:Tec-Monterrey/projectresults

From 2011.igem.org

(Difference between revisions)
Line 583: Line 583:
<br>
<br>
<br>
<br>
-
     <p class="textojustif"> There are several tecniques to prove the successful transportation of CelD and SacC into the outer membrane of <i>E. coli</i>. In this project, SDS-PAGE of entire cell culture samples, SDS-PAGE of membrane fraction samples, and measurement of enzyme activity of whole-cell-system without any chemical purification operation have been considered to confirm the presence of active enzymes on the external membrane.  
+
     <p class="textojustif"> There are several tecniques to prove the successful transportation of CelD and SacC into the outer membrane of <i>E. coli</i>. In this project, SDS-PAGE of entire cell culture samples, SDS-PAGE of membrane fraction samples, and measurement of enzyme activity of whole-cell-system without any chemical purification operation have been considered in order to confirm the presence of active enzymes on the external membrane of <i>E. coli</i>.  
<br>
<br>
<br>
<br>

Revision as of 10:43, 28 September 2011

wiki

iGEM

 

 



There are several tecniques to prove the successful transportation of CelD and SacC into the outer membrane of E. coli. In this project, SDS-PAGE of entire cell culture samples, SDS-PAGE of membrane fraction samples, and measurement of enzyme activity of whole-cell-system without any chemical purification operation have been considered in order to confirm the presence of active enzymes on the external membrane of E. coli.