Team:Tec-Monterrey/projectnotebook june/day06

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06/June/2011
• miniprep of 22P (103.2 NG/ µ L ) and ( 114 ng / µ L )
• Digestions
• Sterilize material
• Preparation of Km and Ap stock.
• Electrophoresis
• Transformation in the microwave
• 22 P + 2 M -> in a plate with Ap
• 16 K + 20 B + 14 N -> in plates with Km
• We chose one colony of the 22P + 2 M and put it in 10 ml LB media + 100 µg / mL)