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Team:Tec-Monterrey/projectnotebook august/day03
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<p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">modeling</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">modeling</a></p> | ||
<p><a href="https://2011.igem.org/Team:Tec-Monterrey/safetypage">safety</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/safetypage">safety</a></p> | ||
| - | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human | + | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human practice</a></p> |
<p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook">notebook</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook">notebook</a></p> | ||
<p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults">results</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults">results</a></p> | ||
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<br> | <br> | ||
| - | + | <p class="justtext"> | |
| - | < | + | 03/August/2011 |
| - | + | </br> | |
| + | • Sergio gave us some recommendations about bacteria induction. | ||
| + | </br> | ||
| + | • Min inoculum 13L, 8G, 2M, 22P, sac and 24C to have an stock of cells and | ||
| + | minipreps of construct B | ||
| + | </br> | ||
| + | • Inoculum of DH5-α, sac, pET12a to verify the restriction site of EcoRI. | ||
| + | </br> | ||
| + | • IPTG induction of 6 different strains: C41, C43, BW27783, XLIBlue, BL21 and | ||
| + | Rosseta gami. | ||
| + | </br> | ||
| + | • We pick 2 colonies of each strain for overnight | ||
| + | </br> | ||
| + | • Minipreps of yesterday’s stock | ||
| + | </br> | ||
| + | • Digestion with EcoRI and PstI forward electrophoresis. | ||
| + | </p> | ||
Latest revision as of 00:35, 18 October 2011
03/August/2011 • Sergio gave us some recommendations about bacteria induction. • Min inoculum 13L, 8G, 2M, 22P, sac and 24C to have an stock of cells and minipreps of construct B • Inoculum of DH5-α, sac, pET12a to verify the restriction site of EcoRI. • IPTG induction of 6 different strains: C41, C43, BW27783, XLIBlue, BL21 and Rosseta gami. • We pick 2 colonies of each strain for overnight • Minipreps of yesterday’s stock • Digestion with EcoRI and PstI forward electrophoresis.
