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Team:Tec-Monterrey/projectnotebook august/day03
From 2011.igem.org
(Difference between revisions)
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<br> | <br> | ||
| - | + | 03/August/2011 | |
| - | + | </br> | |
| + | • Sergio gave us some recommendations about bacteria induction. | ||
| + | </br> | ||
| + | • Min inoculum 13L, 8G, 2M, 22P, sac and 24C to have an stock of cells and | ||
| + | minipreps of construct B | ||
| + | </br> | ||
| + | • Inoculum of DH5-α, sac, pET12a to verify the restriction site of EcoRI. | ||
| + | </br> | ||
| + | • IPTG induction of 6 different strains: C41, C43, BW27783, XLIBlue, BL21 and | ||
| + | Rosseta gami. | ||
| + | </br> | ||
| + | • We pick 2 colonies of each strain for overnight | ||
| + | </br> | ||
| + | • Minipreps of yesterday’s stock | ||
| + | </br> | ||
| + | • Digestion with EcoRI and PstI forward electrophoresis. | ||
| + | </p> | ||
Revision as of 12:24, 28 September 2011
03/August/2011 • Sergio gave us some recommendations about bacteria induction. • Min inoculum 13L, 8G, 2M, 22P, sac and 24C to have an stock of cells and minipreps of construct B • Inoculum of DH5-α, sac, pET12a to verify the restriction site of EcoRI. • IPTG induction of 6 different strains: C41, C43, BW27783, XLIBlue, BL21 and Rosseta gami. • We pick 2 colonies of each strain for overnight • Minipreps of yesterday’s stock • Digestion with EcoRI and PstI forward electrophoresis.
