Team:TU Munich/lab/safety

From 2011.igem.org

Revision as of 08:52, 10 July 2011

1. Would any of your project ideas raise safety issues in terms of:

• researcher safety,
• public safety, or
• environmental safety?

The project ideas of the TU Munich Team 2011 do not raise any more safety issues than those which have to be considered in every biotechnological work involving genetics and microbiology. All team members had to participate in a safety briefing, where we learned handling biological material, aspects on chemicals and the circumstances and protocols at the lab we work in (these may differ from institute to institute). Even though most of us have worked in laboratories before, there are some aspects you can’t hear enough of. In general, the researcher should wear a labcoat, safety glasses and gloves and one mustn't drink, eat or smoke whilst working at the bench. The most important part, however, is that everybody should know at any point of the his work, what he is doing, with what parts and chemicals he is working and how to handle them safely. The lab we work in is classified as BSL1 (biosafety level 1), according to the European Union Directive 2000/54/EG and the German "Gesetz zur Regelung der Gentechnik (GenTG)" (law for the regulation of genetic engineering, text in German only). This means that no devices should be harmful to the researchers if they act corresponding to the general precautionary measures. The most harmful substance in the lab is CyberGreen which is used for staining agarosegels after DNA digestion and separation (used a lot in cloning steps). Here everybody has to be careful, switch gloves everytime he touched something containing CyberGreen and in general be responsible and tidy when working with CyberGreen. Our e.coli strain (xxxxxx) is derived from e.coli xxxx and is modified so it is not harmful to humans. Since nothing from the lab is taken into public and stays inside there should be no safety issues considering public or environmental safety. Used e.coli cultures and waste containing biologic material is autoclaved before throwing away. This ensures that no gentetically modified material can reach the outside of the lab.

Our finished construct itself, the optogenetical AND-Gate, does not pose a threat of any kind. Its only purpose is to controll gene expression in immobilized cells in a spatiotemporal manner. A deliberate missuse of this construct is not possible. This also applies for all intermediate constructs.

2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,

• did you document these issues in the Registry?
• how did you manage to handle the safety issue?
• How could other teams learn from your experience?

None of our BioBrick parts are harmful to humans or the environment. We are working with the red-light sensor and the blue-light sensor combined in an AND Gate. None of those parts should survive outside the lab.

3. Is there a local biosafety group, committee, or review board at your institution?

• If yes, what does your local biosafety group think about your project?
• If no, which specific biosafety rules or guidelines do you have to consider in your country?

Every department at TU Munich needs a safety delegate, in our case Helene Budjarek: She doesn't have any objections against the project due to safety issues. In general, working with genetically modified organisms in Germany is regulated by the '"Gesetz zur Regelung der Gentechnik (GenTG)" (law for the regulation of gentetic engineering, see above).

4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

The easiest way to increase safety when working with BioBricks is to prevent the uncontrolled multiplication and spreading of parts. This can be acchieved by...

• ... the use uncommon restriction enzymes
• ... not using parts containing infectious DNA in combination with parts that can multiply and spread without the help of a host organism (transposons,…)