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Team:SouthBend-Mishawaka-HS-2/Notebook
From 2011.igem.org
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==Notebook== | ==Notebook== | ||
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| + | 05/19/11 Agenda | ||
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| + | (1) Obtained a vial of 2mL of TOP-10 electro competant E. coli cells 3-16-11 (44-0003/793762) | ||
| + | (2) Added 50 microliters of BIO-RAD transformation solution (Control number 31000008916 recd 1-17-11 GT) | ||
| + | (3) Added 2 microliters of DNA from Registry plate 2 5b | ||
| + | (4) Incubate on ice 5 minutes. | ||
| + | (5) Heat shocked 40 degrees C for 50 seconds | ||
| + | (6) Incubuated 4 degrees C for 50 seconds | ||
| + | (7) Added 1mL LB 5-17-11 D.G. | ||
| + | (8) Plate 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "who") | ||
Revision as of 21:43, 19 May 2011
This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. |  |
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Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) | File:SouthBend-Mishawaka-HS-2 team.png Your team picture |
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| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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Notebook
05/19/11 Agenda
(1) Obtained a vial of 2mL of TOP-10 electro competant E. coli cells 3-16-11 (44-0003/793762) (2) Added 50 microliters of BIO-RAD transformation solution (Control number 31000008916 recd 1-17-11 GT) (3) Added 2 microliters of DNA from Registry plate 2 5b (4) Incubate on ice 5 minutes. (5) Heat shocked 40 degrees C for 50 seconds (6) Incubuated 4 degrees C for 50 seconds (7) Added 1mL LB 5-17-11 D.G. (8) Plate 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "who")
