http://2011.igem.org/wiki/index.php?title=Team:Sevilla&feed=atom&action=historyTeam:Sevilla - Revision history2024-03-28T20:06:48ZRevision history for this page on the wikiMediaWiki 1.16.0http://2011.igem.org/wiki/index.php?title=Team:Sevilla&diff=161998&oldid=prevRobertmm at 22:44, 21 September 20112011-09-21T22:44:31Z<p></p>
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</table>Robertmmhttp://2011.igem.org/wiki/index.php?title=Team:Sevilla&diff=161988&oldid=prevRobertmm at 22:44, 21 September 20112011-09-21T22:44:12Z<p></p>
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</table>Robertmmhttp://2011.igem.org/wiki/index.php?title=Team:Sevilla&diff=158299&oldid=prevPcamino at 19:31, 21 September 20112011-09-21T19:31:10Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">Parts submitted</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">We're done</ins></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </html>{{:Team:Sevilla/Templates/hr|colour=#FFB880|class=blog}}<html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </html>{{:Team:Sevilla/Templates/hr|colour=#FFB880|class=blog}}<html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="by"> Posted by <a href="https://2011.igem.org/User:Pcamino">Pablo Camino</a> 20/09/2011</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="by"> Posted by <a href="https://2011.igem.org/User:Pcamino">Pablo Camino</a> 20/09/2011</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After several intense months of work preparing this project very hopefully, finally we have finished Arcanum Project's wiki development and this is the result. We hope you enjoy it as much as we have enjoyed doing it. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After several intense months of work preparing this project very hopefully, finally we have finished Arcanum Project's wiki development and this is the result. We hope you enjoy it as much as we have enjoyed doing it. </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">/</del>p>We would like you to visit all our sections and if you have any inquiries, you can also be in contact with us by facebook, twitter or mail. We will be happy answering your questions or taking your suggestions into account.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>We would like you to visit all our sections and if you have any inquiries, you can also be in contact with us by facebook, twitter or mail. We will be happy answering your questions or taking your suggestions into account.</p></div></td></tr>
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</table>Pcaminohttp://2011.igem.org/wiki/index.php?title=Team:Sevilla&diff=158291&oldid=prevPcamino: Undo revision 158240 by Pcamino (talk)2011-09-21T19:30:42Z<p>Undo revision 158240 by <a href="/Special:Contributions/Pcamino" title="Special:Contributions/Pcamino">Pcamino</a> (<a href="/wiki/index.php?title=User_talk:Pcamino&action=edit&redlink=1" class="new" title="User talk:Pcamino (page does not exist)">talk</a>)</p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </html>{{:Team:Sevilla/Templates/hr|colour=#FFB880|class=blog}}<html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </html>{{:Team:Sevilla/Templates/hr|colour=#FFB880|class=blog}}<html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="by"> Posted by <a href="https://2011.igem.org/User:Pcamino">Pablo Camino</a> 20/09/2011</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="by"> Posted by <a href="https://2011.igem.org/User:Pcamino">Pablo Camino</a> 20/09/2011</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <<del class="diffchange diffchange-inline">p>After several intense months of work preparing this project very hopefully, finally we have finished Arcanum Project's wiki development and this is the result. We hope you enjoy it as much as we have enjoyed doing it.</</del>p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><p>We would like you to visit all our sections and if you have any inquiries, you can also be in contact with us by facebook, twitter or mail. We will be happy answering your questions or taking your suggestions into account.</p></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>See you soon!</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">After several intense months of work preparing this project very hopefully, finally we have finished Arcanum Project's wiki development and this is the result. We hope you enjoy it as much as we have enjoyed doing it. </p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></p>We would like you to visit all our sections and if you have any inquiries, you can also be in contact with us by facebook, twitter or mail. We will be happy answering your questions or taking your suggestions into account.</p></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To achieve our goal we are going to</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To achieve our goal we are going to <ins class="diffchange diffchange-inline"><strong>define a standard of programming modularized biological circuits</strong>, where a community of bacteria -from different but interconnected strains- form a physically independent module. Combining different modules in this way makes it possible to recycle strains and substances. To implement this system, the <strong>key is to standardise the inter-modular communication substances</strong>. This is exactly what we are going to do: we are going to define a substance, which we have named the Ubbit Standard (from Universal BioBit), that will be the element of communication between modules. Just like that, <strong>any research group could use the modules created by any other group and combine them</strong> with their own without the necessity of knowing how it internally works, just by using the Ubbit standard.</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p>Arcanum News</p></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p class="by"> Posted by <a href="https://2011.igem.org/User:Robertmm">Roberto Martín</a> 27/07/2011</p></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img width='125px' height='125px' style="float:left;" src="http://institucional.us.es/igembiouse/images/news.png" /></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> Hi all! I'm glad to announce this new section, we will post events related to our lab, the software we are developing among other things. I hope this section will give you more information about us and about our project, and if you want to contact with us, just send us an email to <a href="mailto:contacto@arcanumproject.com ">contacto@arcanumproject.com </a> or follow us in <a href="www.twitter.com/igemsevilla11">Twitter</a> or look for us in <a href="http://www.facebook.com/pages/Sevilla-iGEM2011-Acanum-Project/187115714638929">Facebook</a></ins></div></td></tr>
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</table>Pcaminohttp://2011.igem.org/wiki/index.php?title=Team:Sevilla&diff=158240&oldid=prevPcamino at 19:27, 21 September 20112011-09-21T19:27:35Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:27, 21 September 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="by"> Posted by <a href="https://2011.igem.org/User:Pcamino">Pablo Camino</a> 20/09/2011</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="by"> Posted by <a href="https://2011.igem.org/User:Pcamino">Pablo Camino</a> 20/09/2011</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <<ins class="diffchange diffchange-inline">p>After several intense months of work preparing this project very hopefully, finally we have finished Arcanum Project's wiki development and this is the result. We hope you enjoy it as much as we have enjoyed doing it.</</ins>p></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>We would like you to visit all our sections and if you have any inquiries, you can also be in contact with us by facebook, twitter or mail. We will be happy answering your questions or taking your suggestions into account.</p></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">After several intense months of work preparing this project very hopefully, finally we have finished Arcanum Project's wiki development and this is the result. We hope you enjoy it as much as we have enjoyed doing it. </p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>See you soon!</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"></p>We would like you to visit all our sections and if you have any inquiries, you can also be in contact with us by facebook, twitter or mail. We will be happy answering your questions or taking your suggestions into account.</p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To achieve our goal we are going to <del class="diffchange diffchange-inline"><strong>define a standard of programming modularized biological circuits</strong>, where a community of bacteria -from different but interconnected strains- form a physically independent module. Combining different modules in this way makes it possible to recycle strains and substances. To implement this system, the <strong>key is to standardise the inter-modular communication substances</strong>. This is exactly what we are going to do: we are going to define a substance, which we have named the Ubbit Standard (from Universal BioBit), that will be the element of communication between modules. Just like that, <strong>any research group could use the modules created by any other group and combine them</strong> with their own without the necessity of knowing how it internally works, just by using the Ubbit standard.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To achieve our goal we are going to</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <p class="by"> Posted by <a href="https://2011.igem.org/User:Robertmm">Roberto Martín</a> 27/07/2011</p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> Hi all! I'm glad to announce this new section, we will post events related to our lab, the software we are developing among other things. I hope this section will give you more information about us and about our project, and if you want to contact with us, just send us an email to <a href="mailto:contacto@arcanumproject.com ">contacto@arcanumproject.com </a> or follow us in <a href="www.twitter.com/igemsevilla11">Twitter</a> or look for us in <a href="http://www.facebook.com/pages/Sevilla-iGEM2011-Acanum-Project/187115714638929">Facebook</a></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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</table>Pcaminohttp://2011.igem.org/wiki/index.php?title=Team:Sevilla&diff=158145&oldid=prevPcamino at 19:20, 21 September 20112011-09-21T19:20:42Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img style="z-index:-5;margin-top:-30px;" src="https://static.igem.org/mediawiki/2011/6/68/Arca.png" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img style="z-index:-5;margin-top:-30px;" src="https://static.igem.org/mediawiki/2011/6/68/Arca.png" /></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div id="post4"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <div class="title"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <p>Parts submitted</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </html>{{:Team:Sevilla/Templates/hr|colour=#FFB880|class=blog}}<html> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <p class="by"> Posted by <a href="https://2011.igem.org/User:Pcamino">Pablo Camino</a> 20/09/2011</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <br></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <div class="postbody"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">After several intense months of work preparing this project very hopefully, finally we have finished Arcanum Project's wiki development and this is the result. We hope you enjoy it as much as we have enjoyed doing it. </p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></p>We would like you to visit all our sections and if you have any inquiries, you can also be in contact with us by facebook, twitter or mail. We will be happy answering your questions or taking your suggestions into account.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>See you soon!</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </p></ins></div></td></tr>
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</table>Pcaminohttp://2011.igem.org/wiki/index.php?title=Team:Sevilla&diff=157785&oldid=prevElena moreno at 18:56, 21 September 20112011-09-21T18:56:58Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:56, 21 September 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p><strong>K514000</strong>: F2620 has been ligated to I732019 so that our cells can express lacZ when they detect C6-3-oxo-HSL (the quorum sensing signal produced by luxI). This way they would turn blue in an XGal-agar plate or turn yellow a liquid medium with ONPG, that's how we've tested them.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p><strong>K514000</strong>: F2620 has been ligated to I732019 so that our cells can express lacZ when they detect C6-3-oxo-HSL (the quorum sensing signal produced by luxI). This way they would turn blue in an XGal-agar plate or turn yellow a liquid medium with ONPG, that's how we've tested them.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>http://partsregistry.org/Part:BBa_K514000</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline"><a href="</ins>http://partsregistry.org/Part:BBa_K514000<ins class="diffchange diffchange-inline">"> BBa_K514000 </a></ins></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><strong>K514001</strong>: we've built a signal transductor with F2620 and C0070. The total construction is F2620+B0034+C0070+B0015, but sadly we weren't able to insert it in pSB1C3, so it's in a plasmid with kanamicine resistance. This part would detect C6-3-oxo-HSL and produce C4-HSL in response. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><strong>K514001</strong>: we've built a signal transductor with F2620 and C0070. The total construction is F2620+B0034+C0070+B0015, but sadly we weren't able to insert it in pSB1C3, so it's in a plasmid with kanamicine resistance. This part would detect C6-3-oxo-HSL and produce C4-HSL in response. </p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>http://partsregistry.org/Part:<del class="diffchange diffchange-inline">BBa_K514000</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline"><a href="</ins>http://partsregistry.org/Part:<ins class="diffchange diffchange-inline">BBa_K514001"> BBa_K514001 </a></ins></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><strong>K514002</strong>: it's a composition of J23119 + I1466 + R0071 in pSB1A2. It works as a receptor for C4-HSL (the quorum sensing signal produced bu RhlI) and activates a specific promotor behind which you can place any gene you want to express conditionally.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><strong>K514002</strong>: it's a composition of J23119 + I1466 + R0071 in pSB1A2. It works as a receptor for C4-HSL (the quorum sensing signal produced bu RhlI) and activates a specific promotor behind which you can place any gene you want to express conditionally.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>http://partsregistry.org/Part:<del class="diffchange diffchange-inline">BBa_K514000</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline"><a href="</ins>http://partsregistry.org/Part:<ins class="diffchange diffchange-inline">BBa_K514002"> BBa_K514002 </a></ins></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>If you want more information don't hesitate to contact us. There will be more information in the parts registry, but we're still working on it :)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>If you want more information don't hesitate to contact us. There will be more information in the parts registry, but we're still working on it :)</div></td></tr>
</table>Elena morenohttp://2011.igem.org/wiki/index.php?title=Team:Sevilla&diff=157737&oldid=prevElena moreno at 18:54, 21 September 20112011-09-21T18:54:40Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:54, 21 September 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Our parts are on their way to the MIT, accross the Atlantic ocean! Hope they have a nice trip and get there in good conditions. We wish we could've sent many exotic new biobricks, but ligations didn't go as well as expected, so we're finally sending three parts, and only one of them is in pSB1C3. Anyway, here you have a summary: </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Our parts are on their way to the MIT, accross the Atlantic ocean! Hope they have a nice trip and get there in good conditions. We wish we could've sent many exotic new biobricks, but ligations didn't go as well as expected, so we're finally sending three parts, and only one of them is in pSB1C3. Anyway, here you have a summary: </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>K514000: F2620 has been ligated to I732019 so that our cells can express lacZ when they detect C6-3-oxo-HSL (the quorum sensing signal produced by luxI). This way they would turn blue in an XGal-agar plate or turn yellow a liquid medium with ONPG, that's how we've tested them.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p<ins class="diffchange diffchange-inline">><strong</ins>>K514000<ins class="diffchange diffchange-inline"></strong></ins>: F2620 has been ligated to I732019 so that our cells can express lacZ when they detect C6-3-oxo-HSL (the quorum sensing signal produced by luxI). This way they would turn blue in an XGal-agar plate or turn yellow a liquid medium with ONPG, that's how we've tested them.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>K514001: we've built a signal transductor with F2620 and C0070. The total construction is F2620+B0034+C0070+B0015, but sadly we weren't able to insert it in pSB1C3, so it's in a plasmid with kanamicine resistance. This part would detect C6-3-oxo-HSL and produce C4-HSL in response. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p<ins class="diffchange diffchange-inline">>http://partsregistry.org/Part:BBa_K514000</p></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>K514002: it's a composition of J23119 + I1466 + R0071 in pSB1A2. It works as a receptor for C4-HSL (the quorum sensing signal produced bu RhlI) and activates a specific promotor behind which you can place any gene you want to express conditionally.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p><strong</ins>>K514001<ins class="diffchange diffchange-inline"></strong></ins>: we've built a signal transductor with F2620 and C0070. The total construction is F2620+B0034+C0070+B0015, but sadly we weren't able to insert it in pSB1C3, so it's in a plasmid with kanamicine resistance. This part would detect C6-3-oxo-HSL and produce C4-HSL in response. </p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p<ins class="diffchange diffchange-inline">>http://partsregistry.org/Part:BBa_K514000</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p><strong</ins>>K514002<ins class="diffchange diffchange-inline"></strong></ins>: it's a composition of J23119 + I1466 + R0071 in pSB1A2. It works as a receptor for C4-HSL (the quorum sensing signal produced bu RhlI) and activates a specific promotor behind which you can place any gene you want to express conditionally.<ins class="diffchange diffchange-inline"></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>http://partsregistry.org/Part:BBa_K514000</ins></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>If you want more information don't hesitate to contact us. There will be more information in the parts registry, but we're still working on it :)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>If you want more information don't hesitate to contact us. There will be more information in the parts registry, but we're still working on it :)</div></td></tr>
</table>Elena morenohttp://2011.igem.org/wiki/index.php?title=Team:Sevilla&diff=143674&oldid=prevRobertmm at 15:08, 20 September 20112011-09-20T15:08:02Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Parts submitted</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Parts submitted</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </html>{{:Team:Sevilla/Templates/hr|colour=#FFB880|class=blog}}<html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </html>{{:Team:Sevilla/Templates/hr|colour=#FFB880|class=blog}}<html> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p class="by"> Posted by <a href="https://2011.igem.org/User:elena_moreno">Elena Moreno</a> <del class="diffchange diffchange-inline">27</del>/<del class="diffchange diffchange-inline">07</del>/2011</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p class="by"> Posted by <a href="https://2011.igem.org/User:elena_moreno">Elena Moreno</a> <ins class="diffchange diffchange-inline">20</ins>/<ins class="diffchange diffchange-inline">09</ins>/2011</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
</table>Robertmmhttp://2011.igem.org/wiki/index.php?title=Team:Sevilla&diff=141236&oldid=prevRobertmm at 10:07, 20 September 20112011-09-20T10:07:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#menu</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#menu</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> height:<del class="diffchange diffchange-inline">1670px</del>;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> height:<ins class="diffchange diffchange-inline">1470px</ins>;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}</div></td></tr>
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</table>Robertmm