Team:SJTU-BioX-Shanghai/Project/Subproject1-1
From 2011.igem.org
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When rare tRNA<sup>Arg</sup>-AGG is not over-expressed, RFP expression is hindered. When tRNA<sup>Arg</sup>-AGG is over-expressed, this tRNA can recognize the AGG codon on the mRNA so a large amount of RFP is produced. | When rare tRNA<sup>Arg</sup>-AGG is not over-expressed, RFP expression is hindered. When tRNA<sup>Arg</sup>-AGG is over-expressed, this tRNA can recognize the AGG codon on the mRNA so a large amount of RFP is produced. | ||
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===Result=== | ===Result=== | ||
Revision as of 19:51, 28 October 2011
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Rare tRNA AmountDesignIn this part we have overexpressed rare tRNAArg-AGG in the cell. The rare tRNA can recognize AGG codon on the mRNA. tRNAArg-AGG(BBa_K567001): tRNAArg-AGG is over expressed under the control of trc promoter (induced by IPTG). This rare tRNAArg can be charged with Arg by native Arginyl-tRNA Synthetase(ArgRS) in E.coli. RFP-6AGG(BBa_K567017): we have inserted 6 AGG codons after the start codon ATG in the RFP gene. ActionWhen rare tRNAArg-AGG is not over-expressed, RFP expression is hindered. When tRNAArg-AGG is over-expressed, this tRNA can recognize the AGG codon on the mRNA so a large amount of RFP is produced.
ResultRFP has been largely produced in cells overexpressing tRNAArg. No RFP can be observed in cells without rare tRNA overexpression. We have successfully controlled protein expression by controlling rare tRNA amount.
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