Team:SJTU-BioX-Shanghai

From 2011.igem.org

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Developed tRNA and aminoacyl tRNA synthetase (aaRS) from the natural biological system into the <a href="/Team:SJTU-BioX-Shanghai/Parts/Data_Page"> Modulator, a controlling device for protein biosynthesis</a>. The function of this device has been fully characterized. Brand-new features of the device are also discovered.</p>
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<b>Gold Medal</b> and <b>Best New BioBrick Part or Device, Engineered</b>: BBa_K567011 & BBa_K567012 at Asia Jamboree. Advancing to World Champion!</p>
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Constructed 4 <a href="/Team:SJTU-BioX-Shanghai/Parts">Modulators</a> (including 8 biobricks) and 13 <a href="/Team:SJTU-BioX-Shanghai/Parts">Reporter</a> biobricks in our 3 subprojedts to test our controlling system. Create parts, including our favorite parts have been sent to Registry.</p>
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Constructed three Codon Switches that controls protein biosynthesis, Rare-Codon Switch, Stop-Codon Switch and Initial-Codon Switch. </p>
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Modified pre-existing reporter genes <a href="/Team:SJTU-BioX-Shanghai/Parts">luciferase</a> and <a href="/Team:SJTU-BioX-Shanghai/Parts">RFP</a> and created a series of new reporter genes. These reporters have been excellent devices in characterizing our system. </p>
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Used Rare-Codon Switch to turn up/down protein biosynthesis by controlling rare tRNA amount, aaRS and the number of rare codons. </p>
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Built our Rare-Codon Switch <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling" ><i>in silic</i></a> and demonstrated <b>the reliability of the model</b>through our experiment results. The data in silic and data experimental matches well.</p>
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Built our Rare-Codon Switch <i>in silic</i> and demonstrated the reliability of the model through our experiment results.
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Analyzed the structure of 18 aaRS with <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling_2">structural biological methods</a>. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability. This work can be used to expand our device aaRS Modulator. </p>
 
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Designed two different <a href="/Team:SJTU-BioX-Shanghai/Project/Application">models</a> for how our device can be applied to <b>controlling metabolic networks</b>, which expands the regulating tools for synthetic biology. </p>
 
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Developed two brand-new ways that <a href="/Team:SJTU-BioX-Shanghai/Project/Application">facilitate the study of protein structure</a>. One for incorporating point mutation into protein. The other for producing truncated proteins to help study the important protein domains. </p>
 
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Design our Human Practice Project: <a href="/Team:SJTU-BioX-Shanghai/Human_practice">T-PostCards</a> to promote our views on synthetic biology through communications and connections.
 
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Attended the <b>2011 China Meet Up</b> and presented our project idea to other iGEMer and advisors. </p>
 
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Revision as of 02:07, 29 October 2011

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    The Project: Codon Switch Controlling Protein Biosynthesis

    SJTU-BioX-Shanghai iGEM team is designing a set of Codon-Switches that regulate target protein biosynthesis (translation).

    In our Rare-Codon Switch, the translation of the protein can be finely turned up/down with the control of rare tRNA amount, aaRS that charges the rare tRNA and rare codons.

    Besides, our device can be made into switches that can be turned on/off without background noise in two ways. One is to use stop codon as the controlling element, the Stop-Codon Switch. The other is to use any codon but the original start codon to initiate translation, the Initial-Codon Switch.

    Project

    Our design has expanded the regulating tools for synthetic biology and introduced brand-new methods for protein function analysis. See more about project applications, click here.

    Team
    Parts
    Human practice
    Achievements

    right Gold Medal and Best New BioBrick Part or Device, Engineered: BBa_K567011 & BBa_K567012 at Asia Jamboree. Advancing to World Champion!

    right Constructed three Codon Switches that controls protein biosynthesis, Rare-Codon Switch, Stop-Codon Switch and Initial-Codon Switch.

    right Used Rare-Codon Switch to turn up/down protein biosynthesis by controlling rare tRNA amount, aaRS and the number of rare codons.

    right Built our Rare-Codon Switch in silic and demonstrated the reliability of the model through our experiment results.

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    right

    right

    right

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