Team:Penn State/Research

From 2011.igem.org

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<p> Throughout the summer we connected with other iGEM teams interested in radiation-related projects. The <a href="https://2011.igem.org/Team:NYC_Wetware">NYC Wetware</a> team in particular was studying the effects of genes cloned from the organism Deinococcus radiodurans on radiation resistance in E.coli. We were given the honor of helping them characterize one of their strains TS TR, a strain capable of producing the molecule trypanothione.</br>
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Using the gamma facilities provided by the Radiation Science and Engineering Center at Penn state we were able to carry out tests of gamma irradiation of their strain and subsequently measure cell viability.
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Aliquots of culture (5ml each) inoculated with the TS TR strain were added to tubes which were irradiated by a cobalt-60 gamma source at a dose rate of 25 gys/min for total doses ranging from 0-800 gys. After irradiation 0.02 ml of the irradiated sample was inoculated into 5 ml of SOB media. The OD<sub>600</sub> was measured 12 hours later. The results are shown below.
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Revision as of 03:38, 28 September 2011

Skip to achievements Skip to experimental results Skip to parts Skip to dosimeter design Skip to collaboration PSU iGEM 2011 Home
PSU iGEM 2011 Home Wet lab Research Human Practices Results
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Achievements

We did lots of great things! Check it!

Registered Team, had (too much) fun throughout the summer
Attended the Jamboree
At least one submitted and characterized part
Demonstrated part works as expected
Characterized one new part and entered the data in the “Main Page” section of that Part’s/Device’s Registry entry
Worked towards improving the function of an existing BioBrick Part
Helped another team (see our collaboration with NYC wetware below!)
New approach to human practices (Watch our Human Practices Video and learn about our workshop here)

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Experimental Results

Testing of Or as a Promoter

The backbone of the radiation sensor in our project relies on the operating region Or, and the two promoters one on each end of Or. In order to test the function ability of the of the pr promoter we attached a part called mcherry, which has rfp to the promoter. We used the TECAN in our lab to measure the fluorescence of our samples over time. The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional.

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Parts

Favorites

We need to put these here asap.

List

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Dosimeter Design

We will put stuff here

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Collaboration

Throughout the summer we connected with other iGEM teams interested in radiation-related projects. The NYC Wetware team in particular was studying the effects of genes cloned from the organism Deinococcus radiodurans on radiation resistance in E.coli. We were given the honor of helping them characterize one of their strains TS TR, a strain capable of producing the molecule trypanothione.

Using the gamma facilities provided by the Radiation Science and Engineering Center at Penn state we were able to carry out tests of gamma irradiation of their strain and subsequently measure cell viability.

Aliquots of culture (5ml each) inoculated with the TS TR strain were added to tubes which were irradiated by a cobalt-60 gamma source at a dose rate of 25 gys/min for total doses ranging from 0-800 gys. After irradiation 0.02 ml of the irradiated sample was inoculated into 5 ml of SOB media. The OD600 was measured 12 hours later. The results are shown below.