Team:Peking S/lab/notebook/sr

From 2011.igem.org

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===7.9===
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0 :19  Checked plates.
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0 :19  Gel extraction & PCR of lacI-LVA-suffix.
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14:00  Digestion of 1-1G and lacI PCR product(second round) with X&P.
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14:00  Colony pick of 1-1G plates.
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18:30  Electrophoresis for digestion products(1-1G XP fragment;lacI PCR product digested with X&P).
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23:45  Ligation of 1-1G and lacI-suffix fragments started.
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===7.10===

Revision as of 04:10, 2 October 2011


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  • Rui Sun's Notebook


summary

In this summer, I mainly focused on the construction of the microbial population density balancer. In addition, I also carried out the assay for orthogonality of positive regulating signaling molecules.

Contents

July

Mon Tue Wed Thu Fri Sat Sun
- - - - 1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
- - - - - - -

[TOP]

7.7

ABBREVIATIONS:

Restriction endonuleases:

E=EcoRI X=XbaI S=SpeI P=PstI


17:30 Digestion of B0034(1-2M) with S&P and 4-12I with X&P started.

     Fragment lengths: B0034  around 2000bps
                       4-12I  756 bps
     PCR of terminator-less version of Ptet-rhlI and lacI(I732011)-LVA
     Electroperesis:
     Gel 1 Well 2-4: Marker_4-12I_1-2M
     Gel 2 Well 2-5: Marker_rhlI_rhlI_rhlI
     Gel 3 Well 1-6: Marker_lacI_lacI_ _ _lacI_lacI
     All checked right.
   
     Gel extraction of the above DNA fragments.
     Ligation of B0034(SP digested)+4-12I(XP digested).

7.8

12:30 Transformation of the ligation product(1-2M+4-12I) finished. 12:30 Gel extraction of PCR product (LacI-LVA) finished.

17:30 PCR for lacI-tag from I732100 started.

19:30 Transformation of 2-10G finished.

19:30 PCR failed. Repeated with the same system &condition and got the right string.

7.9

0 :19 Checked plates.

0 :19 Gel extraction & PCR of lacI-LVA-suffix.

14:00 Digestion of 1-1G and lacI PCR product(second round) with X&P.

14:00 Colony pick of 1-1G plates.

18:30 Electrophoresis for digestion products(1-1G XP fragment;lacI PCR product digested with X&P).

23:45 Ligation of 1-1G and lacI-suffix fragments started.

7.10

7.12

7.13

7.14

7.15

7.16

7.17

7.18

7.19

7.20

7.21

7.22

7.23

7.24

7.25

7.26

7.27

7.28

7.29

7.30

7.31

August

Mon Tue Wed Thu Fri Sat Sun
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31 - - - -

[TOP]

8.1

8.2

8.4

8.5

8.6

8.7

8.8

8.9

8.10

8.11

8.12

8.13

8.14

8.15

8.16

8.17

8.18

8.19

8.20

8.21

8.22

8.23

8.25

8.26

8.27

8.30

8.31

September

Mon Tue Wed Thu Fri Sat Sun
- - 1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 - --

[TOP]

9.1

9.2

9.3

9.6

9.7

9.8

9.9

9.12

9.13

9.14

9.15

9.16

9.17

9.21

9.22

9.23

9.24

9.25

9.26

9.27

9.28

9.29

9.30

October

Mon Tue Wed Thu Fri Sat Sun
- - - - 1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 - - - - - -

[TOP]

10.1

10.3

10.4

10.5

10.7

10.8

10.9

10.10

10.11

10.12

10.13

10.15

10.16-10.21

10.21-10.25



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