Team:Peking S/data

Data Page

Data For Our Favorite New Parts

1. [http://partsregistry.org/Part:BBa_K581003 Main Page] - SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2), BBa_K581003: This is the conjugate part of ptsG2-gfp.

1. [http://partsregistry.org/Part:BBa_K581004 Main Page] - ptsG2-GFP (ptsG2 5'UTR fused with gfp), BBa_K581004: This is the conjugate part of the small RNA regulator sgrS2.

1. [http://partsregistry.org/Part:BBa_K581019 Main Page] - rhl repressible promoter-gfp-ssrA degradation tag, BBa_K581019: We ceated the rhl repressible promoter by positioning the rhl-box between and partially overlapping consensus -35 and -10 hexamers with 18 bp between the hexamers. When C4-HSL represents and binds to RhlR proteins, RhlR will bind to its DNA binding site, the rhl box, thus to reduce the accessibility of RNA polymerases to this promoter, repressing the transcription initiation. A GFP reporter with C-terminus fused ssrA degredation tag was used to report the transcriptional activity at the promoters.

Data For Our New Parts

1. [http://partsregistry.org/Part:BBa_K581000 Main Page] - SgrS1+Terminator (small RNA regulator, conjugate part of ptsG1), BBa_K581000: SgrS1 is the G178C mutant of SgrS(wt) and the conjugate part of ptsG1 in our comparator device.

1. [http://partsregistry.org/Part:BBa_K581001 Main Page] - ptsG1-GFP (ptsG1 5' UTR fused with gfp), BBa_K581001: This is the conjugate part of the small RNA sgrS1.

1. [http://partsregistry.org/Part:BBa_K581002 Main Page] - RBS+cinI+Terminator, BBa_K581002: This is a cinI protein generator.

1. [http://partsregistry.org/Part:BBa_K581005 Main Page] - Pc+sgrS(wt)+Terminator (small RNA regulator, conjugate part of ptsG(wt)), BBa_K581005: SgrS(sugar transport-related sRNA)(wt) is a small RNA regulator that help cells recover from glucose-phosphate stress by base pairing with ptsG(wt) mRNA. SgrS regulates ptsG mRNA by short, imperfect base-pairing interactions; as a result, the expression of PtsG is repressed.

1. [http://partsregistry.org/Part:BBa_K581006 Main Page] - Pc+ptsG(wt)-GFP (ptsG(wt) 5' UTR fused with gfp), BBa_K581006: PtsG is a glucose permease which is subordinate to phosphotransferase system and serves as a transporter. Here,we studied this mRNA perform the conjugate part of the small RNA regulator sgrS(wt). ptsG mRNA is regulated by SgrS by short, imperfect base-pairing interactions, and its expression is thus repressed.