Team:Peking R/Notebook/YXW

From 2011.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 12: Line 12:
left:91px;
left:91px;
top:325px;
top:325px;
-
width:640px;
+
width:533px;
-
height:9452px;
+
height:650px;
z-index:1;
z-index:1;
font-family: "Comic Sans MS", cursive;
font-family: "Comic Sans MS", cursive;
font-size: 14px;
font-size: 14px;
-
color: #333;
+
color: #000;
text-align: justify;
text-align: justify;
 +
font-style: normal;
 +
font-weight: normal;
}
}
.notbookmaintitle {
.notbookmaintitle {
Line 24: Line 26:
font-family: "Arial Black", Gadget, sans-serif;
font-family: "Arial Black", Gadget, sans-serif;
color: #666;
color: #666;
-
font-weight: bold;
 
}
}
.name {
.name {
font-size: 16px;
font-size: 16px;
-
font-weight: bold;
 
font-family: "Comic Sans MS", cursive;
font-family: "Comic Sans MS", cursive;
color: #333;
color: #333;
Line 99: Line 99:
#apDiv3 {
#apDiv3 {
position:absolute;
position:absolute;
-
left:36px;
+
left:41px;
-
top:291px;
+
top:275px;
width:540px;
width:540px;
height:28px;
height:28px;
Line 122: Line 122:
a:active {
a:active {
text-decoration: none;
text-decoration: none;
 +
}
 +
.clickteam #apDiv1 table tr th p {
 +
font-family: Georgia, "Times New Roman", Times, serif;
 +
font-style:normal;
 +
}
 +
asdf {
 +
font-weight: normal;
 +
}
 +
qqq {
 +
font-weight: bold;
 +
}
 +
a {
 +
font-weight: bold;
 +
}
 +
a {
 +
font-weight: bold;
 +
}
 +
a {
 +
font-weight: bold;
 +
}
 +
th {
 +
font-family: Georgia, "Times New Roman", Times, serif;
 +
font-size: 14px;
 +
font-style: normal;
 +
font-weight: normal;
 +
font-variant: normal;
 +
color: #000;
 +
}
 +
.clickteam #apDiv1 table {
 +
color: #000;
 +
}
 +
p {
 +
color: #000;
 +
}
 +
a {
 +
color: #000;
 +
}
 +
th {
 +
font-family: Georgia, "Times New Roman", Times, serif;
 +
font-size: 14px;
 +
font-style: normal;
 +
font-weight: normal;
 +
font-variant: normal;
 +
color: #000;
}
}
</style>
</style>
Line 128: Line 172:
<body class="clickteam">
<body class="clickteam">
<div id="apDiv1">
<div id="apDiv1">
-
   <table width="600" border="0" cellspacing="0" cellpadding="0">
+
   <table width="648" border="0" cellspacing="0" cellpadding="0">
     <tr>
     <tr>
-
       <th height="38" colspan="5" bgcolor="#DCF8A9" class="notbookmaintitle" scope="col">Xiaowei Yan's Notebook</th>
+
       <th height="38" colspan="5" bgcolor="#FFFFFF" class="notbookmaintitle" scope="col">Yan Xiaowei's Notebook</th>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th colspan="5" bgcolor="#DCF8A9" class="notbookmaintitle" scope="col"><hr /></th>
+
       <th colspan="5" bgcolor="#FFFFFF" class="notbookmaintitle" scope="col"><hr /></th>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th width="16" height="94" bgcolor="#DCF8A9" scope="row"><p>&nbsp;</p>
+
       <th width="3" height="70" bgcolor="#FFFFFF" scope="row"><p>&nbsp;</p>
       <p>&nbsp;</p></th>
       <p>&nbsp;</p></th>
-
       <th colspan="2" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3">I am in charge of the construction of c-di-GMP and theophylline ribozymes, focusing on the response properties of these ribozymes to corresponding ligands ---- c-di-GMP &amp; theophylline. I am also the designer of our team's shirt.</p></th>
+
       <th colspan="2" bgcolor="#FFFFFF" scope="row"><p> I am in charge of the construction of c-di-GMP and theophylline ribozymes, focusing on the response properties of these ribozymes to corresponding ligands ---- c-di-GMP &amp; theophylline. I am also the designer of our team's shirt. </p></th>
-
       <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Peking_R/Team/YXW"><img src="https://static.igem.org/mediawiki/2011/c/cd/PekingR_xiaowei11.jpg" alt="" width="100" height="140" /></a><a href="https://2011.igem.org/Team:Peking_R/Team/ZMQ"></a><a href="https://2011.igem.org/Team:Peking_R/Team/ZZR"></a></p></th>
+
       <th width="54" rowspan="6" bgcolor="#FFFFFF" scope="row"><p>&nbsp;</p></th>
-
       <th width="20" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
       <th width="145" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th rowspan="40" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
       <th rowspan="40" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
-
       <th width="29" height="29" bgcolor="#DCF8A9" scope="row"><img src="https://static.igem.org/mediawiki/2011/0/0c/PekingR_PDF_download_logo.png" alt="" width="30" height="29" /></th>
+
       <th width="59" height="66" bgcolor="#FFFFFF" scope="row"><img src="https://static.igem.org/mediawiki/2011/0/0c/PekingR_PDF_download_logo.png" alt="" width="30" height="29" /></th>
-
       <th width="410" nowrap="nowrap" bgcolor="#DCF8A9" class="download" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook/ZZR" class="clickteam">Download her note</a></th>
+
       <th width="387" nowrap="nowrap" bgcolor="#FFFFFF" class="download" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook/ZZR" class="clickteam">Download her note</a>book</th>
-
       <th rowspan="40" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
       <th rowspan="40" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th height="23" colspan="2" bgcolor="#DCF8A9" class="name" scope="row">Contents:</th>
+
       <th height="23" colspan="2" bgcolor="#FFFFFF" class="name" scope="row">Contents:</th>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th height="8" colspan="2" bgcolor="#DCF8A9" scope="row"><hr /></th>
+
       <th height="8" colspan="2" bgcolor="#FFFFFF" scope="row"><hr /></th>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th colspan="2" bgcolor="#DCF8A9" class="July"> <a href="#July" class="clickteam">*July,2011</a></th>
+
        
     </tr>
     </tr>
     <tr>
     <tr>
-
      <th colspan="2" bgcolor="#DCF8A9" class="July" scope="row"><a href="#Augest" class="clickteam">*Augest,2011</a></th>
+
       <th colspan="3" align="left" bgcolor="#FFFFFF" scope="row"><p><strong>07.01—07.07</strong><br />
-
    </tr>
+
·Positive transformation of the parts which  may be used in our project from 2011 Distribution.</p>
-
    <tr>
+
        <p><strong>07.08—07.16</strong><br />
-
       <th colspan="3" bgcolor="#DCF8A9" class="July" scope="row"><a href="#September" class="clickteam">*September,2011</a></th>
+
           ·Primary construction of the part about  c-di-GMP responsive ribozyme.<br />
-
    </tr>
+
           At first, assembly the c-di-GMP responsive  ribozyme with GFP using OE-PCR (pBAD+c-di-GMP ribozyme+GFP+ter B0015). Because  of the delayed primers, the problems of DNA purification kit and gel extraction  kit,  the ignorance of the question on  fidelity of Taq enzyme, these experiments have been done many times, so they’re  finished a little late. Whatever, WT、M6、M8 and GFP have been OK now.<br />
-
    <tr>
+
          ·The growth curve determination of DH5α in media of LB.</p>
-
      <th colspan="3" bgcolor="#DCF8A9" class="July" scope="row"><a href="#October" class="clickteam">*October,2011</a></th>
+
        <p><strong>07.17—07.24</strong><br />
-
    </tr>
+
          ·Assembly the c-di-GMP responsive ribozyme  (WT/M6/M8) with GFP using OE-PCR successfully.<br />
-
    <tr>
+
          ·Some comparisons between different kits.</p>
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
+
        <p><strong>07.25—07.31</strong><br />
-
    </tr>
+
           ·Violacein<br />
-
    <tr>
+
           Separation of individual genes in the  violacein metabolic pathway ——vioA/B/D/E. <br />
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
           The primers of vioC seem to have a prefer  annealing ability with a particular sequence within vioC.<br />
-
    </tr>
+
           Testing and optimizing the protocols of PCR.</p>
-
    <tr>
+
         <p><strong>08.01—08.14</strong><br />
-
      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">July
+
           ·c-di-GMP ribozyme construction.<br />
-
      <a name="July" id="July"></a>        <hr /></th>
+
           Because the GFP fused with c-di-GMP responsive  ribozyme (WT) couldn’t be ligated with pBAD and terminator B0015 for some  reason. I have tried another approach——Gibson assembly and finally the sequence  has been correct.</p>
-
    </tr>
+
         <p><strong>08.15—09.05    with the help of GONG Yan</strong><br />
-
    <tr>
+
           ·With the help of GONG Yan, we construct  c-di-GMP responsive ribozyme (M6/M8) regulatory system (pBAD+c-di-GMP  M6/M8+GFP+ter B0015) using Gibson assembly.<br />
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
+
           ·Construction of a new theophylline responsive  ribozyme regulatory system.(pBAD+RBS+theo Parental/theo Th2P6/theo B11+GFP+ter)<br />
-
        <tr>
+
           NOTE:<br />
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
+
           Theo Th2P6  is a reconstructed Group I intron with an anti-theophylline aptamer.<br />
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
+
          Theo  Parental is the original Group I intron.<br />
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
+
          Theo B11  is another reconstructed Group I intron which shows no activity in vivo.</p>
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
+
         <p><strong>09.06—10.04</strong><br />
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
+
           ·Constructing the part of vioC (ter  B0015+pBAD+theophylline riboswitch N8-3/1G1+vioC+ter B0015).<br />
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
+
       Because the product of vioC isn’t sufficient  for digesting of restriction enzyme, I insert the vioC into pUC18 first.</p></th>
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
+
-
        </tr>
+
-
        <tr>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><span class="sunday">-</span></td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d701" class="clickteam">1</a></td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d702" class="clickteam">2</a></td>
+
-
        </tr>
+
-
        <tr class="clickteam">
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#703" class="clickteam">3</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d704" class="clickteam">4</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d705" class="clickteam">5</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d707" class="clickteam">7</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d708" class="clickteam">8</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d707" class="clickteam">9</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d710" class="clickteam">10</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d711" class="clickteam">11</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d712" class="clickteam">12</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d713" class="clickteam">13</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d714" class="clickteam">14</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d716" class="clickteam">16</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
           <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d717" class="clickteam">17</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d718" class="clickteam">18</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d719" class="clickteam">19</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d720" class="clickteam">20</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d721" class="clickteam">21</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d722" class="clickteam">22</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d723" class="clickteam">23</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d724" class="clickteam">24</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d725" class="clickteam">25</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d726" class="clickteam">26</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d727" class="clickteam">27</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d728" class="clickteam">28</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d729" class="clickteam">29</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d730" class="clickteam">30</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d731" class="clickteam">31</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
           <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
        </tr>
+
-
      </table></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th height="16" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><a href="#topjuly" class="clickteam">[Top]</a></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th height="31" colspan="3" bgcolor="#DCF8A9" class="download" scope="row">&nbsp;</th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">7.1--7.7<a name="d701" id="d701"></a><a name="d702" id="d702"></a><a name="d703" id="d703"></a><a name="d704" id="d704"></a><a name="d705" id="d705"></a><a name="d706" id="d706"></a><a name="d707" id="d707"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p class="selfstatement3"><span class="selfstatement3">7.8-7.14<a name="d708" id="d708"></a><a name="d709" id="d709"></a><a name="d710" id="d710"></a><a name="d711" id="d711"></a><a name="d712" id="d712"></a><a name="d713" id="d713"></a><a name="d714" id="d714"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p class="selfstatement3"><span class="selfstatement3">7.15-7.20<a name="d715" id="d715"></a><a name="d716" id="d716"></a><a name="d717" id="d717"></a><a name="d718" id="d718"></a><a name="d719" id="d719"></a><a name="d720" id="d720"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p><span class="selfstatement3">7.21-7.28<a name="d721" id="d721"></a><a name="d722" id="d722"></a><a name="d723" id="d723"></a><a name="d724" id="d724"></a><a name="d725" id="d725"></a><a name="d726" id="d726"></a><a name="d727" id="d727"></a><a name="d728" id="d728"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p class="selfstatement3"><span class="selfstatement3">7.29-7.31<a name="d729" id="d729"></a><a name="d730" id="d730"></a><a name="d731" id="d731"></a></span></p>
+
-
      <p>&nbsp;</p></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">Augest<a name="Augest" id="Augest"></a></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
+
-
        <tr>
+
-
           <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
+
-
           <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
+
-
           <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
+
-
           <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
+
-
         </tr>
+
-
        <tr>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d801" class="clickteam">1</a></td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d802" class="clickteam">2</a></td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d803" class="clickteam">3</a></td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d804" class="clickteam">4</a></td>
+
-
          <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d805" class="clickteam">5</a></td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d806" class="clickteam">6</a></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d807" class="clickteam" >7</a></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d808" class="clickteam">8</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d809" class="clickteam">9</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d810" class="clickteam">10</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d811" class="clickteam">11</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d812" class="clickteam">12</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d813" class="clickteam">13</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d814"class="clickteam" >14</a></span></th>
+
-
           <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d815" class="clickteam">15</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d816" class="clickteam">16</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d817"class="clickteam">17</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d818"class="clickteam">18</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d819"class="clickteam">19</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d820"class="clickteam">20</a></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d821"class="clickteam">21</a></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d822"class="clickteam">22</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d823"class="clickteam">23</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d824"class="clickteam">24</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d825"class="clickteam">25</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d826"class="clickteam">26</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d827"class="clickteam">27</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d828"class="clickteam">28</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d829"class="clickteam">29</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d830"class="clickteam">30</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d831"class="clickteam">31</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9">-</td>
+
-
           <td align="center" bgcolor="#DCF8A9">-</td>
+
-
        </tr>
+
-
      </table></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><hr /></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#topaugest" class="clickteam">[Top]</a></span></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">8.1-8.5<a name="d801" id="d801"></a><a name="d802" id="d802"></a><a name="d803" id="d803"></a><a name="d804" id="d804"></a></span><a name="d805" id="d805"></a></p>
+
-
        <p>&nbsp;</p>
+
-
<p class="selfstatement3"><span class="selfstatement3">8.6-8.13<a name="d806" id="d806"></a><a name="d807" id="d807"></a><a name="d808" id="d808"></a><a name="d809" id="d809"></a><a name="d810" id="d810"></a><a name="d811" id="d811"></a></span><a name="d812" id="d812"></a><a name="d813" id="d813"></a></p>
+
-
<p>&nbsp;</p>
+
-
<p class="selfstatement3">&nbsp;</p>
+
-
         <p class="selfstatement3"><span class="selfstatement3">8.14-8.18<a name="d814" id="d814"></a><a name="d815" id="d815"></a><a name="d816" id="d816"></a><a name="d817" id="d817"></a><a name="d818" id="d818"></a></span></p>
+
-
        <p class="selfstatement3"><span class="selfstatement3">8.19-8.25<a name="d819" id="d819"></a><a name="d820" id="d820"></a><a name="d821" id="d821"></a><a name="d822" id="d822"></a><a name="d823" id="d823"></a><a name="d824" id="d824"></a><a name="d825" id="d825"></a></span></p>
+
-
        <p class="selfstatement3"><span class="selfstatement3">8.26-8.31<a name="d826" id="d826"></a><a name="d827" id="d827"></a><a name="d828" id="d828"></a><a name="d829" id="d829"></a><a name="d830" id="d830"></a><a name="d831" id="d831"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">September<a name="September" id="September"></a></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row"><hr />
+
-
</th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th height="71" colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
+
-
        <tr>
+
-
           <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
+
-
        </tr>
+
-
        <tr>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d901"class="clickteam">1</a></td>
+
-
           <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d902"class="clickteam">2</a></td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d903"class="clickteam">3</a></td>
+
-
        </tr>
+
-
        <tr>
+
-
           <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d904"class="clickteam">4</a></th>
+
-
           <td align="center" bgcolor="#DCF8A9"><a href="#d905"class="clickteam">5</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d906"class="clickteam">6</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d907"class="clickteam">7</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d908"class="clickteam">8</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d909"class="clickteam">9</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d910"class="clickteam">10</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d911"class="clickteam">11</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d912"class="clickteam">12</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d913"class="clickteam">13</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d914"class="clickteam">14</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d915"class="clickteam">15</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d916"class="clickteam">16</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d917"class="clickteam">17</a></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d918"class="clickteam">18</a></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d919"class="clickteam">19</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d920"class="clickteam">20</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d921"class="clickteam">21</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d922"class="clickteam">22</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d923"class="clickteam">23</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d924"class="clickteam">24</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d925"class="clickteam">25</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d926"class="clickteam">26</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d927"class="clickteam">27</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d928"class="clickteam">28</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d929"class="clickteam">29</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d930"class="clickteam">30</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9">-</td>
+
-
        </tr>
+
-
      </table></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><hr /></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#topseptember" class="clickteam">[Top]</a></span></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">9.1-9.8<a name="d901" id="d901"></a><a name="d902" id="d902"></a><a name="d903" id="d903"></a><a name="d904" id="d904"></a><a name="d905" id="d905"></a><a name="d906" id="d906"></a><a name="d907" id="d907"></a><a name="d908" id="d908"></a></span></p>
+
-
        <p class="selfstatement3"><span class="selfstatement3">9.9-9.15<a name="d909" id="d909"></a><a name="d910" id="d910"></a><a name="d911" id="d911"></a><a name="d912" id="d912"></a><a name="d913" id="d913"></a><a name="d914" id="d914"></a><a name="d915" id="d915"></a></span></p>
+
-
        <p class="selfstatement3"><span class="selfstatement3">9.16-9.22<a name="d916" id="d916"></a><a name="d917" id="d917"></a><a name="d918" id="d918"></a><a name="d919" id="d919"></a><a name="d920" id="d920"></a><a name="d921" id="d921"></a><a name="d922" id="d922"></a></span></p>
+
-
         <p class="selfstatement3"><span class="selfstatement3">9.23-9.30<a name="d923" id="d923"></a><a name="d924" id="d924"></a><a name="d925" id="d925"></a><a name="d926" id="d926"></a><a name="d927" id="d927"></a><a name="d928" id="d928"></a><a name="d929" id="d929"></a><a name="d930" id="d930"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">October<a name="October" id="October"></a></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
+
-
        <tr>
+
-
           <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
+
-
        </tr>
+
-
        <tr>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td valign="middle" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d1001" class="clickteam"><span class="clickteam">1</span></a></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d1002" class="clickteam">2</a></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d1003" class="clickteam">3</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d1004" class="clickteam">4</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9">5</td>
+
-
          <td align="center" bgcolor="#DCF8A9">6</td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">7</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9">8</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row">9</th>
+
-
          <td align="center" bgcolor="#DCF8A9">10</td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">11</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">12</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">13</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">14</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">15</span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday">16</span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">17</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">18</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9">19</td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">20</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">21</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">22</span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday">23</span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">24</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">25</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">26</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">27</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">28</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9">29</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday">30</span></th>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">31</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
        </tr>
+
-
       </table></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><hr /></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#topoctober" class="clickteam">[Top]</a></span></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p><span class="selfstatement3"><span class="selfstatement3">10.1-10.4</span><a name="d1001" id="d1001"></a><a name="d1002" id="d1002"></a><a name="d1003" id="d1003"></a><a name="d1004" id="d1004"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p></th>
+
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th colspan="3" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
       <th colspan="3" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th colspan="3" bgcolor="#DCF8A9" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Team/YXW" class="clickteam">==click here to her page==</a></th>
+
       <th colspan="3" bgcolor="#FFFFFF" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Team/LYP" class="clickteam">==click here to his page==</a></th>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th colspan="3" bgcolor="#DCF8A9" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook">==click here to return==</a></th>
+
       <th colspan="3" bgcolor="#FFFFFF" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook">==click here to return==</a></th>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th colspan="3" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
       <th colspan="3" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
     </tr>
     </tr>
   </table>
   </table>
Line 547: Line 250:
     <th width="46" class="clickteam" scope="col">&gt;&gt;</th>
     <th width="46" class="clickteam" scope="col">&gt;&gt;</th>
       <th width="146" class="clickteam" scope="col"><span class="clickteam"><a href="https://2011.igem.org/Team:Peking_R/Notebook" class="clickteam">Personal Note</a></span></th>
       <th width="146" class="clickteam" scope="col"><span class="clickteam"><a href="https://2011.igem.org/Team:Peking_R/Notebook" class="clickteam">Personal Note</a></span></th>
-
       <th width="37" class="clickteam" scope="col">&gt;&gt;</th>
+
       <th width="35" class="clickteam" scope="col">&gt;&gt;</th>
-
       <th width="250" align="left" class="clickteam" scope="col"><a href="https://2011.igem.org/Team:Peking_R/Notebook/YXW" class="clickteam">Xiaowei Yan</a><a name="topjuly" id="topjuly"></a><a name="topaugest" id="topaugest"></a><a name="topseptember" id="topseptember"></a><a name="topoctober" id="topoctober"></a></th>
+
       <th width="252" align="left" class="clickteam" scope="col"><a href="https://2011.igem.org/Team:Peking_R/Notebook/LYP" class="clickteam">Yan Xiaowei</a><a name="topjuly" id="topjuly"></a><a name="topaugest" id="topaugest"></a><a name="topseptember" id="topseptember"></a><a name="topoctober" id="topoctober"></a></th>
     </tr>
     </tr>
   </table>
   </table>

Latest revision as of 01:17, 6 October 2011

Template:Https://2011.igem.org/Team:Peking R/bannerhidden Template:Https://2011.igem.org/Team:Peking R/back2 Template:Https://2011.igem.org/Team:Peking R/Notebookbackground 无标题文档

Yan Xiaowei's Notebook

 

 

I am in charge of the construction of c-di-GMP and theophylline ribozymes, focusing on the response properties of these ribozymes to corresponding ligands ---- c-di-GMP & theophylline. I am also the designer of our team's shirt.

 

 
  Download her notebook  
Contents:

07.01—07.07
·Positive transformation of the parts which may be used in our project from 2011 Distribution.

07.08—07.16
·Primary construction of the part about c-di-GMP responsive ribozyme.
At first, assembly the c-di-GMP responsive ribozyme with GFP using OE-PCR (pBAD+c-di-GMP ribozyme+GFP+ter B0015). Because of the delayed primers, the problems of DNA purification kit and gel extraction kit,  the ignorance of the question on fidelity of Taq enzyme, these experiments have been done many times, so they’re finished a little late. Whatever, WT、M6、M8 and GFP have been OK now.
·The growth curve determination of DH5α in media of LB.

07.17—07.24
·Assembly the c-di-GMP responsive ribozyme (WT/M6/M8) with GFP using OE-PCR successfully.
·Some comparisons between different kits.

07.25—07.31
·Violacein
Separation of individual genes in the violacein metabolic pathway ——vioA/B/D/E.
The primers of vioC seem to have a prefer annealing ability with a particular sequence within vioC.
Testing and optimizing the protocols of PCR.

08.01—08.14
·c-di-GMP ribozyme construction.
Because the GFP fused with c-di-GMP responsive ribozyme (WT) couldn’t be ligated with pBAD and terminator B0015 for some reason. I have tried another approach——Gibson assembly and finally the sequence has been correct.

08.15—09.05   with the help of GONG Yan
·With the help of GONG Yan, we construct c-di-GMP responsive ribozyme (M6/M8) regulatory system (pBAD+c-di-GMP M6/M8+GFP+ter B0015) using Gibson assembly.
·Construction of a new theophylline responsive ribozyme regulatory system.(pBAD+RBS+theo Parental/theo Th2P6/theo B11+GFP+ter)
NOTE:
Theo Th2P6 is a reconstructed Group I intron with an anti-theophylline aptamer.
Theo Parental is the original Group I intron.
Theo B11 is another reconstructed Group I intron which shows no activity in vivo.

09.06—10.04
·Constructing the part of vioC (ter B0015+pBAD+theophylline riboswitch N8-3/1G1+vioC+ter B0015).
Because the product of vioC isn’t sufficient for digesting of restriction enzyme, I insert the vioC into pUC18 first.

 
==click here to his page==
==click here to return==